Accurate, Model-Based Tuning of Synthetic Gene Expression Using Introns in

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Synthetic biology is gradually expanding our capability to engineer biology through rational genetic engineering of synthetic gene expression systems. These developments are already paving the way for the accelerated study of biology and applying engineered biological systems to major environmental and health problems. However, our capacity to intelligently modify and control gene expression depends on our ability to apply a broad range of genetic regulators in the engineering process. Here we show that Introns, pivotal regulators of Eukaryotic gene expression, can be rationally engineered to control a synthetic gene expression system of a Eukaryote. We developed a unique reporter-based system to evaluate the effects of engineering splicing in synthetic biology and show that the entire intron repertoire of S. cerevisiae can be accurately used to rationally engineer gene expression. Our results provide both a proof-of-concept for the integration of splicing into synthetic biology designs and a model that can be used by the scientific community for integrating splicing into their own designs. Following the extensive use of transcriptional (promoter) and translational (UTR) elements in synthetic constructs, our results introduce a new major regulatory system, splicing, that can be used to rationally engineer genetic systems.

Vyšlo v časopise: Accurate, Model-Based Tuning of Synthetic Gene Expression Using Introns in. PLoS Genet 10(6): e32767. doi:10.1371/journal.pgen.1004407
Kategorie: Research Article
prolekare.web.journal.doi_sk: https://doi.org/10.1371/journal.pgen.1004407

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