CPF-Associated Phosphatase Activity Opposes Condensin-Mediated Chromosome Condensation

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Failure to properly condense chromosomes prior to their segregation in mitosis can lead to genome instability. The evolutionary-conserved condensin complex is key to the condensation process but the molecular mechanisms underlying its localization pattern on chromosomes remain unclear. Previous observations showed that the localization of condensin is intimately linked to regions of high transcription, although, somewhat paradoxically, its association with chromatin is disrupted by a processive polymerase activity. Here we identify several RNA processing factors as negative regulators of condensin in fission yeast. Two of these factors associate with PP1 phosphatase as an independent entity within the Cleavage and Polyadenylation Factor (CPF), a complex key for 3′ end RNA processing. Lack of this module induces only minor and context-dependent effects on gene expression. Our data suggest that this module helps maintaining the proper level of phosphatase activity within the CPF and thereby opposes the function of condensin in mitotic chromosome condensation.

Vyšlo v časopise: CPF-Associated Phosphatase Activity Opposes Condensin-Mediated Chromosome Condensation. PLoS Genet 10(6): e32767. doi:10.1371/journal.pgen.1004415
Kategorie: Research Article
prolekare.web.journal.doi_sk: https://doi.org/10.1371/journal.pgen.1004415

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