Pervasive Divergence of Transcriptional Gene Regulation in Caenorhabditis Nematodes

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Given the importance of gene expression changes in evolution, a better understanding of how they accumulate is desirable. However, gene regulation is a complex biochemical process and it is not a priori clear whether general trends even exist. We systematically addressed this question by testing, in C. elegans, the functions of regulatory elements of eight different genes from four other nematodes. We saw rampant variation in gene regulatory mechanisms, even between closely related species. While the differences were usually seen in a relatively small number of cells, there was a discernible trend – there were many more instances of gain, rather than loss of expression, compared to patterns directed by the C. elegans cis elements. Finally, the recurrence of ectopic expression in the same cells suggests that the paths open to evolution may be constrained by the composition of regulatory elements. We view these patterns as a reflection of general mechanisms of gene regulatory evolution and suggest that these can be refined, and others discovered, using systematic functional tests.

Vyšlo v časopise: Pervasive Divergence of Transcriptional Gene Regulation in Caenorhabditis Nematodes. PLoS Genet 10(6): e32767. doi:10.1371/journal.pgen.1004435
Kategorie: Research Article
prolekare.web.journal.doi_sk: https://doi.org/10.1371/journal.pgen.1004435

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