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The RNAPII-CTD Maintains Genome Integrity through Inhibition of Retrotransposon Gene Expression and Transposition
RNA Polymerase II (RNAPII) is the enzyme responsible for the transcription of all protein-coding genes, many non-coding genes and retrotransposons. RNAPII has a unique C-terminal domain (CTD) that is composed of repeats of a seven amino acid sequence and is conserved across species. The CTD functions as a recruiting platform for regulatory and RNA processing factors, making the CTD a master orchestrator of transcription. Mutants containing reduced CTD length show defects in steady state transcription during growth in normal conditions, as well as defects in induced gene expression. However, how CTD length affects retrotransposon gene expression remains to be investigated. Here, we uncovered a direct role for CTD length in limiting retrotransposon gene expression and mobility, revealing a new role for the RNAPII-CTD in maintaining genome integrity.
Vyšlo v časopise: The RNAPII-CTD Maintains Genome Integrity through Inhibition of Retrotransposon Gene Expression and Transposition. PLoS Genet 11(10): e32767. doi:10.1371/journal.pgen.1005608
Kategorie: Research Article
prolekare.web.journal.doi_sk: https://doi.org/10.1371/journal.pgen.1005608Souhrn
RNA Polymerase II (RNAPII) is the enzyme responsible for the transcription of all protein-coding genes, many non-coding genes and retrotransposons. RNAPII has a unique C-terminal domain (CTD) that is composed of repeats of a seven amino acid sequence and is conserved across species. The CTD functions as a recruiting platform for regulatory and RNA processing factors, making the CTD a master orchestrator of transcription. Mutants containing reduced CTD length show defects in steady state transcription during growth in normal conditions, as well as defects in induced gene expression. However, how CTD length affects retrotransposon gene expression remains to be investigated. Here, we uncovered a direct role for CTD length in limiting retrotransposon gene expression and mobility, revealing a new role for the RNAPII-CTD in maintaining genome integrity.
Zdroje
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