Functional Activation of the Flagellar Type III Secretion Export Apparatus

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Bacteria build needle-like injectsomes to secrete toxins into host cells and build propeller-like flagella to swim through their environment using a molecular machine called the type III secretion system (T3SS). Both the injectisome and the flagellum are large self-assembling complexes and regulation of the T3SS ensures that proteins are secreted sequentially for proper structure and function. Here we report genetic and cytological data that the SwrB protein of Bacillus subtilis helps the base of the flagellum adopt a completed conformation which in turn activates the enclosed T3SS to export proteins for the next stage of flagellar assembly. Thus SwrB presents a novel mechanism to supervise an early structural checkpoint regulating machine assembly. Targeting functional regulators like SwrB could inhibit T3SS-based strategies of pathogens.

Vyšlo v časopise: Functional Activation of the Flagellar Type III Secretion Export Apparatus. PLoS Genet 11(8): e32767. doi:10.1371/journal.pgen.1005443
Kategorie: Research Article
prolekare.web.journal.doi_sk: https://doi.org/10.1371/journal.pgen.1005443

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