piRNAs Are Associated with Diverse Transgenerational Effects on Gene and Transposon Expression in a Hybrid Dysgenic Syndrome of .

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Transposable elements (TEs) are selfish elements that copy themselves. More than half of the human genome is comprised of such elements. Studies in the fruit flies Drosophila melanogaster and D. virilis have been important in demonstrating a role for RNA silencing by PIWI-interacting RNAs (piRNAs) in protecting the genome against these harmful elements. These small RNAs are capable of recognizing TE mRNAs and mediating their destruction. They are also transmitted by the female germline to offspring in order to maintain a stable genome across generations. When males carrying a particular TE family are crossed with females lacking the element, the mother is unable to provide genome defense via complementary piRNAs that target the element. This leads to excess TE activation in the germline and sterility, a phenomenon known as hybrid dysgenesis. In this article we characterize the genomic landscape of TE destabilization that occurs in dysgenic crosses of D. virilis. We demonstrate that this mobilization is associated with an increased level of germline TE expression that persists through adulthood. In addition, we find that TE activation is associated with diverse effects on normal gene expression that are also mediated by piRNAs.

Vyšlo v časopise: piRNAs Are Associated with Diverse Transgenerational Effects on Gene and Transposon Expression in a Hybrid Dysgenic Syndrome of .. PLoS Genet 11(8): e32767. doi:10.1371/journal.pgen.1005332
Kategorie: Research Article
prolekare.web.journal.doi_sk: https://doi.org/10.1371/journal.pgen.1005332

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