Rescheduling Behavioral Subunits of a Fixed Action Pattern by Genetic Manipulation of Peptidergic Signaling

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In Drosophila, the pupal ecdysis behavioral sequence is composed of three distinct steps: pre-ecdysis, ecdysis, and post-ecdysis. We hypothesize that release of ecdysis-triggering-hormone (ETH) from endocrine Inka cells drives these stereotypical behaviors through sequential activation of peptidergic ETH receptor (ETHR) neuron ensembles in the central nervous system (CNS). There are many questions about how a single hormone orchestrates a stepwise behavioral sequence. Here we present evidence implicating two central ETHR ensembles—kinin and CAMB—causally in pre-ecdysis and ecdysis behaviors. Using calcium imaging, we show a sequential pattern of activity in kinin and CAMB neurons that is temporally correlated with pre-ecdysis and ecdysis behaviors, respectively. Genetic manipulation of kinin and CAMB neurons demonstrates that timing of the behaviors is a function of: 1) sensitivity to the hormone, and 2) upstream inhibitory inputs. These findings provide insights into the molecular bases of behavioral orchestration by central peptidergic ensembles.

Vyšlo v časopise: Rescheduling Behavioral Subunits of a Fixed Action Pattern by Genetic Manipulation of Peptidergic Signaling. PLoS Genet 11(9): e32767. doi:10.1371/journal.pgen.1005513
Kategorie: Research Article
prolekare.web.journal.doi_sk: https://doi.org/10.1371/journal.pgen.1005513

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