Multicopy Single-Stranded DNA Directs Intestinal Colonization of Enteric Pathogens

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Multicopy single-stranded DNA (msDNA) is a unique molecule consisting of both an RNA and DNA portion. This molecule is produced by a reverse transcriptase and has no known natural function despite more than 30 years of study. We report that msDNA is important for both Salmonella Typhimurium and an enteropathogenic E. coli, two pathogens that cause diarrhea in susceptible hosts, to survive in the intestine. Using mutant strains incapable of producing msDNA, we show that msDNA is needed for Salmonella to grow in the absence of oxygen. Mutants grown in oxygen-deficient conditions have substantial changes in overall protein composition, including numerous proteins known to be important for anaerobic metabolism and growth in the intestine. Our findings link msDNA to the ability of Salmonella to thrive in an oxygen-deficient environment similar to the conditions inside the gut. We report that msDNA regulates the quantity of proteins, the first natural function attributed to this molecule. msDNA may represent a new class of regulatory molecules.

Vyšlo v časopise: Multicopy Single-Stranded DNA Directs Intestinal Colonization of Enteric Pathogens. PLoS Genet 11(9): e32767. doi:10.1371/journal.pgen.1005472
Kategorie: Research Article
prolekare.web.journal.doi_sk: https://doi.org/10.1371/journal.pgen.1005472

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