Ty3 Retrotransposon Hijacks Mating Yeast RNA Processing Bodies to Infect New Genomes

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Cells undergoing changes in gene expression programs such as nutritional deprivation and other stresses exhibit formation of ribonucleoprotein (RNP) complexes. In Saccharomyces cerevisiae, the majority of investigations to date involve analysis of P-body (PB) and stress-granule RNP formation following nutritional stress. Few studies have investigated RNP formation induced by the mating-MAP-kinase pathway. In this study, we examined how this process influences genome stability via control of retrotransposon activation. During the mating response, expression of the retrotransposon Ty3 is induced and Ty3 virus-like particles form in RNP clusters called retrosomes. We show that mating retrosomes contain PB components that are essential for Ty3 expression, re-localization of Ty3 RNA and protein from polysomes into foci, and retrotransposition. In animal germ cell lineages, PB components are found in perinuclear complexes with RNA interference suppressors of retrotransposition. We speculate that when RNA interference is relaxed and retrotransposition is observed, some members of these complexes play positive roles as we observe in budding yeast.

Vyšlo v časopise: Ty3 Retrotransposon Hijacks Mating Yeast RNA Processing Bodies to Infect New Genomes. PLoS Genet 11(9): e32767. doi:10.1371/journal.pgen.1005528
Kategorie: Research Article
prolekare.web.journal.doi_sk: https://doi.org/10.1371/journal.pgen.1005528

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