Caspase-1-Like Regulation of the proPO-System and Role of ppA and Caspase-1-Like Cleaved Peptides from proPO in Innate Immunity

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Melanization is an important reaction in most multicellular organisms, both animals and plants. The initiation steps of this reaction in invertebrates are catalyzed by the prophenoloxidase (proPO) activating system a proteolytic enzyme cascade, which primary function is to recognize cell wall products from microorganisms and respond by activation of the system and generation of immune effector molecules. This cascade requires careful regulation to achieve spatial and temporal control to avoid dangerous side effects. We here show that a Caspase1-like enzyme can inactivate proPO when ppA is not activating the proPO to avoid deleterious effects and further we show for the first time that the N-terminal peptide from ppA cleavage of proPO (activation of proPO) has an important biological function as also the Caspase1 cleaved fragments. Our results also show that Caspase 1-induced inflammatory response is evolutionarily conserved and is linked to melanization.

Vyšlo v časopise: Caspase-1-Like Regulation of the proPO-System and Role of ppA and Caspase-1-Like Cleaved Peptides from proPO in Innate Immunity. PLoS Pathog 10(4): e32767. doi:10.1371/journal.ppat.1004059
Kategorie: Research Article
prolekare.web.journal.doi_sk: https://doi.org/10.1371/journal.ppat.1004059

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