The RhoGAP SPIN6 Associates with SPL11 and OsRac1 and Negatively Regulates Programmed Cell Death and Innate Immunity in Rice

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Rice diseases are the major threat for stable rice production and food security worldwide. Deep understanding of the disease resistance pathway in rice is essential for effective control of the diseases. Although rice contains many E3 ubiquitin ligases, the function of their substrates in immune responses is still not fully understood. We previously characterized U-box E3 ligase SPL11 in rice that is involved in the regulation of cell death, immune responses, and flowering. However, how SPL11 interacts with its substrates to control cell death and immunity is not clear. In this study, we found that the SPL11 interacts with SPIN6, ubiquitinates, and degrades the protein via the 26S proteasome pathway. Both the Spin6 RNAi and mutant plants show enhanced resistance to rice pathogens and activate defense gene expression and ROS generation. Importantly, we found that SPIN6 is the RhoGAP of the small GTPase OsRac1, which is a key component in rice immunity. Our study provides further insights into the relationship between SPIN6 and its interacting proteins SPL11 and OsRac1, and its function in the control of cell death and immunity in rice.

Vyšlo v časopise: The RhoGAP SPIN6 Associates with SPL11 and OsRac1 and Negatively Regulates Programmed Cell Death and Innate Immunity in Rice. PLoS Pathog 11(2): e32767. doi:10.1371/journal.ppat.1004629
Kategorie: Research Article
prolekare.web.journal.doi_sk: https://doi.org/10.1371/journal.ppat.1004629

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