A Novel AT-Rich DNA Recognition Mechanism for Bacterial Xenogeneic Silencer MvaT

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During evolution, the bacteria frequently acquire new genes through horizontal transfer, in order to adapt new environments. However, foreign DNA sequences acquired are more likely to decrease rather than increase the fitness of the recipient bacteria. Therefore, many bacterial genera have evolved unique proteins to selectively repress the transcription of foreign genes. The opportunistic pathogen Pseudomonas aeruginosa is the principal cause of the morbidity and mortality in cystic fibrosis patients and is among the major causes of nosocomial infections. As a xenogeneic silencer, the MvaT protein of P. aeruginosa is a master regulator of horizontally acquired genes including many critical for drug resistance and virulence. Here, we have characterized the DNA sequences preferentially targeted by MvaT, and identified differences in sequence preferences between MvaT and other xenogeneic silencers. The high resolution structure of the DNA-binding domain of MvaT in complex with a high affinity DNA target reveals a novel AT-rich DNA minor groove recognition mechanism, which perfectly explains the characteristic of MvaT’s DNA sequence preferences. Comparison between MvaT and other bacterial xenogeneic silencers demonstrates how unique solutions have been employed by different bacterial genera in distinguishing foreign DNA from DNA of their own genome.

Vyšlo v časopise: A Novel AT-Rich DNA Recognition Mechanism for Bacterial Xenogeneic Silencer MvaT. PLoS Pathog 11(6): e32767. doi:10.1371/journal.ppat.1004967
Kategorie: Research Article
prolekare.web.journal.doi_sk: https://doi.org/10.1371/journal.ppat.1004967

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