HYPER RECOMBINATION1 of the THO/TREX Complex Plays a Role in Controlling Transcription of the Gene in Arabidopsis

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The transcription and export of RNA is a dynamic and highly coordinated process. mRNA species that are selectively mediated by the THO/TRanscription EXport (THO/TREX) complex for their transcription and export remain to be identified. As well, the specific roles of complex components in transcription-coupled export are unclear. We reveal a role for HYPER RECOMBINATION1 (HPR1) [the yeast HYPER RECOMBINATION1 (Hpr1) homolog] in REVERSION-TO-ETHYLENE SENSITIVITY1 (RTE1) transcription elongation but not activation or export, which agrees with the role of yeast Hpr1 in transcription elongation. Defects in the THO/TREX component TEX1 but not the RNA-export TREX-2 component SAC3B also reduced the native RTE1 level. Our data suggest a specific role of the THO/TREX component HPR1 in RTE1 expression. Whether TEX1 is involved in RTE1 transcription or stability remains to be determined. The yeast Sub2 protein is an RNA helicase involved in unwinding the inhibitory structure in the nascent RNA, and SUB2 overexpression suppresses yeast Δhpr1 defects; HPR1 could be involved in expression of selected genes with higher-order structure, where RNA polymerase movement could pause. Studies of the gene structure and transcription activity could shed light on roles of these components in gene expression regulation at the transcription-export level.

Vyšlo v časopise: HYPER RECOMBINATION1 of the THO/TREX Complex Plays a Role in Controlling Transcription of the Gene in Arabidopsis. PLoS Genet 11(2): e32767. doi:10.1371/journal.pgen.1004956
Kategorie: Research Article
prolekare.web.journal.doi_sk: https://doi.org/10.1371/journal.pgen.1004956

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