Genome Sequencing and Comparative Genomics of the Broad Host-Range Pathogen AG8

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The fungus Rhizoctonia solani is divided into several sub-species which cause disease in a range of plant species that includes most major agriculture, forestry and bioenergy species. This study focuses on sub-species AG8 which causes disease of cereals, canola and legumes, and compares its genome to other R. solani sub-species and a wide range of fungal and non-fungal species. R. solani is unusual in that it can possess more than one nucleus per cell. The multiple nuclei and sequence mutations between them made assembly of its genome challenging, and required novel techniques. We observed signs that DNA sequences originating from multiple nuclei in AG8 exhibit a high frequency of single nucleotide polymorphisms (SNPs) and more SNP diversity than most fungal populations. These SNP mutations also have similarities to repeat-induced point mutations (RIP). Moreover in AG8, RIP-like SNPs are not restricted to intergenic regions but are also widely observed in gene-coding regions. This is novel as RIP has previously only been reported in repetitive DNA of distantly-related fungi that have only a single nucleus per cell. We generated a list of 308 genes with similar properties to known plant-disease proteins, in which we found higher rates of non-synonymous mutations than normal.

Vyšlo v časopise: Genome Sequencing and Comparative Genomics of the Broad Host-Range Pathogen AG8. PLoS Genet 10(5): e32767. doi:10.1371/journal.pgen.1004281
Kategorie: Research Article
prolekare.web.journal.doi_sk: https://doi.org/10.1371/journal.pgen.1004281

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