The Vip1 Inositol Polyphosphate Kinase Family Regulates Polarized Growth and Modulates the Microtubule Cytoskeleton in Fungi

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Fungi are an extremely successful and diverse group of organisms ranging from the small single-celled yeasts to the indefinitely growing filamentous fungi. Polarized growth, where growth is restricted to defined regions, leads to the specific cell shape of yeast cells, as well as the very long hyphae of filamentous fungi. Fungal polar growth is controlled by an internal regulatory circuit of which the microtubule cytoskeleton comprises the transport road for numerous cargos needed for polarized growth. However, the microtubule cytoskeleton is not static, but a dynamic structure, which is modulated by microtubule-associated proteins and the interaction with other cellular structures. Our present analysis has identified a new regulator of the microtubule cytoskeleton in the fission yeast S. pombe: a member of the highly conserved Vip1 inositol polyphosphate kinase family. Vip1 proteins have a dual domain structure consisting of an N-terminal kinase domain which synthesizes inositol pyrophosphates and a C-terminal domain, which we show to negatively regulate the kinase output. Our results suggest that modulation of microtubule dynamics is correlated to Vip1 kinase activity. Importantly, polarized growth and microtubule dynamics were also modulated by Vip1 family members in A. nidulans and U. maydis thus uncovering a conserved biological role for inositol pyrophosphates.

Vyšlo v časopise: The Vip1 Inositol Polyphosphate Kinase Family Regulates Polarized Growth and Modulates the Microtubule Cytoskeleton in Fungi. PLoS Genet 10(9): e32767. doi:10.1371/journal.pgen.1004586
Kategorie: Research Article
prolekare.web.journal.doi_sk: https://doi.org/10.1371/journal.pgen.1004586

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