A Cytosine Methytransferase Modulates the Cell Envelope Stress Response in the Cholera Pathogen

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Methylation of DNA is used by numerous organisms to regulate a wide variety of cellular processes, but specific roles for most DNA methyltransferases have not been defined. We studied one such enzyme in Vibrio cholerae, the cholera pathogen, using genome-wide approaches to compare DNA methylation, gene expression, and the sets of genes required or dispensable for growth in bacterial strains that produced or lacked this enzyme. These studies allowed us to identify numerous cellular processes regulated, either directly or indirectly, by this cytosine methyltransferase. In particular, we found that an absence of enzyme activity was associated with reduced levels of a bacterial stress response; consequently, a stress response pathway that is essential in wild type bacteria is not needed for survival of the mutant lacking the methyltransferase. Similar genome-wide analyses can likely to be used to define the cellular roles of many additional uncharacterized DNA methyltransferases.

Vyšlo v časopise: A Cytosine Methytransferase Modulates the Cell Envelope Stress Response in the Cholera Pathogen. PLoS Genet 11(11): e32767. doi:10.1371/journal.pgen.1005666
Kategorie: Research Article
prolekare.web.journal.doi_sk: https://doi.org/10.1371/journal.pgen.1005666

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