Encodes Dual Oxidase, Which Acts with Heme Peroxidase Curly Su to Shape the Adult Wing

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Fruit fly geneticists rely on a handful of dominant mutations that modify adult morphology in a way that is easy to spot, like changing the shape of the fly’s wings, eyes or bristles. One of the first such mutants identified in the early days of fly genetics and to this day likely the most widely used mutation, is Curly, which causes an upward curvature in the adult wings. Despite its importance as a marker, the genetic cause of Curly has remained unknown. Here, we reveal that Curly mutations occur in the gene duox, which encodes a ROS-generating enzyme. ROS once thought to be merely harmful by-products of metabolism, can also have beneficial purposes. Here we provide evidence that Duox generates ROS to help form and stabilize the wings of fruit flies. Furthermore, we identify a second enzyme, Cysu, which uses the ROS generated by Duox to crosslink proteins in the wing, thereby stabilizing and shaping its structure. Duox occurs in numerous organisms, including humans and fulfills a number of other functions, in particular in immunity and pathogen defense. With this new knowledge, Curly mutations will provide an excellent tool to study and understand the roles Duox plays in a variety of biological contexts.

Vyšlo v časopise: Encodes Dual Oxidase, Which Acts with Heme Peroxidase Curly Su to Shape the Adult Wing. PLoS Genet 11(11): e32767. doi:10.1371/journal.pgen.1005625
Kategorie: Research Article
prolekare.web.journal.doi_sk: https://doi.org/10.1371/journal.pgen.1005625

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