R-loops Associated with Triplet Repeat Expansions Promote Gene Silencing in Friedreich Ataxia and Fragile X Syndrome
Friedreich ataxia and Fragile X syndrome are among 40 human diseases associated with expansion of repeated sequences. In both disorders repeat expansion leads to gene silencing, the molecular mechanism of which is not well understood, impeding the development of specific therapies to treat these disorders. It is proposed that formation of unusual DNA structures (RNA/DNA hybrids, or R-loops) over repeat regions may play a role, but their molecular function has not been investigated in vivo. We show that R-loops form on expanded repeats of FXN and FMR1 genes in cells from FRDA and FXS patients. These R-loops are stable, correlate with repressive chromatin marks and hinder FXN transcription in patient cells. We studied the relationship between repressive chromatin and R-loops. Decrease in the amount of repressive chromatin has no effect on R-loop levels. In contrast, increase in the R-loops leads to transcriptional silencing of FXN gene and formation of repressive chromatin, providing a direct molecular link between R-loops and pathology of expansion diseases. This discovery is important for understanding the basic molecular mechanism underlying the pathology of expansion diseases. The ability of R-loops to trigger transcriptional silencing makes them an attractive target for future therapeutic approaches to treat these diseases.
Published in the journal:
R-loops Associated with Triplet Repeat Expansions Promote Gene Silencing in Friedreich Ataxia and Fragile X Syndrome. PLoS Genet 10(5): e32767. doi:10.1371/journal.pgen.1004318
Category:
Research Article
doi:
https://doi.org/10.1371/journal.pgen.1004318
Summary
Friedreich ataxia and Fragile X syndrome are among 40 human diseases associated with expansion of repeated sequences. In both disorders repeat expansion leads to gene silencing, the molecular mechanism of which is not well understood, impeding the development of specific therapies to treat these disorders. It is proposed that formation of unusual DNA structures (RNA/DNA hybrids, or R-loops) over repeat regions may play a role, but their molecular function has not been investigated in vivo. We show that R-loops form on expanded repeats of FXN and FMR1 genes in cells from FRDA and FXS patients. These R-loops are stable, correlate with repressive chromatin marks and hinder FXN transcription in patient cells. We studied the relationship between repressive chromatin and R-loops. Decrease in the amount of repressive chromatin has no effect on R-loop levels. In contrast, increase in the R-loops leads to transcriptional silencing of FXN gene and formation of repressive chromatin, providing a direct molecular link between R-loops and pathology of expansion diseases. This discovery is important for understanding the basic molecular mechanism underlying the pathology of expansion diseases. The ability of R-loops to trigger transcriptional silencing makes them an attractive target for future therapeutic approaches to treat these diseases.
Introduction
Around forty human diseases are associated with expanded repeat sequences [1]. Friedreich ataxia (FRDA) is the most frequent autosomal recessive ataxia (2–4 cases/100,000), caused by a GAA expansion in the first intron of the frataxin (FXN) gene, which encodes a mitochondrial protein involved in iron-sulfur cluster biogenesis [2], [3]. The GAA expansion leads to reduced levels of FXN mRNA and protein [4]–[6]. Several mechanisms mediating FXN transcriptional silencing have been proposed, including the formation of unusual DNA structures (triplex DNA and RNA/DNA hybrids) and repressive heterochromatin over expanded repeats [5]–[10].
RNA/DNA hybrids (R-loops) are formed during transcription, when nascent RNA hybridizes to the DNA template behind the elongating RNA polymerase (Pol II). R-loops are detected in organisms from bacteria to humans and implicated in many processes [11]. In mammalian cells, R-loops were originally discovered in the immuno-globulin class switch regions, essential for generating the antibody diversity in mouse activated B cells [12], [13]. R-loops also accumulate in cells depleted of the key splicing factor SRSF1, resulting in genome instability and appearance of double-strand breaks [14]. Recent studies demonstrated that R-loops are enriched over CpG promoters and may be involved in protection of these regions from DNA methylation and maintaining the hypomethylated state of CpG promoters [15]. We recently showed that R-loops formed over the G-rich pause sites downstream of the polyA signal in human genes are essential for the process of transcriptional termination of RNA Pol II [16]. Furthermore RNA/DNA hybrids are induced at GAA repeats following in vitro transcription and in bacteria [17], [18]. Also R-loops formed on plasmids containing CTG/CAG repeats in E.coli and mini-gene constructs in human cells promoted repeat instability, pointing towards their role in disease pathology [19], [20]. However, the direct involvement of R-loops on endogenous expanded alleles in the pathology of FRDA has not yet been investigated in vivo.
Our study shows that RNA/DNA hybrids (R-loops) form on expanded repeats of endogenous FXN and FMR1 genes, associated with Friedreich ataxia and Fragile X (FXS) disorders, in patient cells. These transcription-dependent R-loops are resistant to cellular degradation and co-localise with repressive H3K9me2 chromatin marks, characteristic of these diseases. Using nascent nuclear run-on analysis we show that R-loops over expanded repeats impede RNA Polymerase II transcription of the FXN gene in patient cells. We investigated the interplay between repressive chromatin marks and R-loops on the FXN gene. We show that a decrease in repressive H3K9me2 chromatin mark has no effect on R-loop levels and FXN transcription. In contrast, increasing R-loop levels leads to transcriptional repression of FXN gene, providing a direct molecular link between R-loops and pathology of FRDA. These data suggest that R-loops formed over expanded repeats act as an initial trigger to promote FXN and FMR1 silencing, and represent a common feature of nucleotide expansion diseases, contributing to their pathology in vivo.
Results
FXN transcriptional initiation and elongation defect in FRDA cells
We examined transcriptional regulation of the FXN gene in immortalized lymphoblastoid cells derived from FRDA patients, where FXN mRNA expression is reduced by ∼80% (Figure 1A–C). Pol II chromatin immuno-precipitation (ChIP) analysis in these cells showed that Pol II is enriched over the exon 1, positioned at the major transcriptional start site (TSS2) in lymphoblasts, correlating with the promoter-specific histone H3 depleted region [4] (Figure 1D, S1). Pol II levels over exon 1 were significantly reduced in FRDA cells. Similarly, using quantitative RT-PCR (RT-qPCR) in three independent control and three FRDA cell lines, a dramatic reduction in nascent RNA was detected over exon 1 in FRDA cells, further confirming a defect in transcription initiation (Figure 1E, S2A and S2B left panels). We also observed ∼10-fold reduction in the nascent RNA downstream of the expansion in regions D–G in FRDA cells. Overall Pol II ChIP and RT-qPCR results suggest transcriptional initiation and elongation defects triggered by expanded repeats, in line with previous reports [4], [6], [21].
R-loops are formed over expanded repeat regions of FXN gene in vivo
Recently we established the DNA immuno-precipitation (DIP) method, which allows detection of R-loops on endogenous human genes in vivo using S9.6 antibody which recognizes RNA/DNA hybrids [16], [22]. Here we employed DIP to investigate R-loop distribution on the FXN gene. As a positive control we used the intron 1 region of the γ-actin gene, where high levels of R-loops are detected [15]. Significantly, we observed ∼3-fold enrichment of R-loops over regions B, C and D in the FXN intron 1 in FRDA cells, compared to control cells (Figure 1F). R-loops were concentrated over the expanded repeat region and were low in the downstream regions E–G. γ-actin R-loop levels were similar in control and FRDA cells (Figure 1F). Similar R-loop enrichment over expanded GAA repeats was detected in two additional independent FRDA cell lines (Figure S2A–B, right panels). Interestingly, when we compared the DIP data from all control and FRDA cell lines we observed that the level of R-loops correlates with expansion length (Figure S2C). To confirm the specificity of the DIP signal, we treated the samples with RNase H, which specifically degrades the RNA in RNA/DNA hybrids, prior to immuno-precipitation. Following RNase H digestion, the signal was strongly reduced for control γ-actin and FXN regions, suggesting that genuine R-loops are formed over the expanded GAA repeats (Figure 1G). High level of R-loops detected in FRDA cells may also suggest that these structures are particularly stable over FXN expanded repeats. Therefore, R-loops could act in cis to affect FXN gene expression in FRDA cells.
R-loops are stable and impede Pol II transcription on FXN gene
To understand the function of R-loops in FRDA pathology, we further characterized these structures over the expanded FXN allele. In particular, we treated cells with the transcriptional inhibitor actinomycin D. Following this treatment for 21 hours we observed an ∼80% reduction in γ-actin nascent RNA and R-loop signal, suggesting that γ-actin R-loops are quickly turned over in the cell (Figure 2A, B). In contrast, although nascent FXN RNA decreased following actinomycin D treatment, no change in R-loop levels was detected. However, we did finally observe a significant decrease in the level of R-loops over expanded repeats following prolonged treatment with actinomycin D for 48 hours (Figure S3). Overall these results suggest that R-loops associated with FXN expanded repeats are resistant to degradation. This may relate to the expanded GAA repeat property of transcriptional repression and repeat instability. We also observed that enrichment of R-loops correlated with highest peaks of the repressive histone modification H3K9me2 over the FXN regions B–D in FRDA cells (Figure 2C and 1F). This suggests that R-loops over expanded GAA repeats may functionally associate with repressive heterochromatin and be involved in mediating transcriptional repression.
Previously we showed that R-loops formed at the 3′ends of human genes promote transcriptional termination of RNA Pol II [16]. We therefore investigated if R-loops over FXN expanded repeats affect Pol II elongation. Here we employed nuclear run-on (NRO) analysis with Br-UTP labelled nucleotide [16], which measures actively transcribing Pol II (Figure 2D), in contrast to ChIP, which detects the total Pol II level on the gene. Using NRO we observed a substantial decrease in active transcription upstream of the GAA expansion (regions in1 and B) in FRDA cells, confirming our Pol II ChIP results (Figure 2E and 1D). In addition, we also detected ∼3-fold reduction in active transcription in FRDA cells over FXN regions D and E, positioned 210 nt and 4.5 kb downstream of expansion, respectively (Figure 2E). This elongation defect is not due to the increased distance caused by GAA expansion (∼3 kb), since we observed no decrease in active transcription between regions D to E, separated by ∼4.3 kb, in both cell lines. This suggests that expanded repeats directly interfere with active Pol II transcription in FRDA cells.
R-loops on expanded repeats of integrated FXN gene are sensitive to RNase H1 over-expression in HEK293 cells
To test if expanded GAA repeats trigger the formation of R-loops in a different genomic location, we employed HEK293 cells, containing a copy of the FXN gene with either six (FXN-Luc) or ∼310 (FXN-GAA-Luc) GAA repeats, fused to the luciferase gene, integrated on chromosome 1, while the endogenous FXN gene lies on chromosome 9 (Figure 3A) [23]. We confirmed the presence of the GAA expansion using PCR on genomic DNA extracted from these cells. As demonstrated in Figure 3B, the FXN-GAA-Luc cell line indeed contains ∼310 expanded repeats. The presence of GAA repeats caused a reduction of ∼37% in FXN-luciferase nascent RNA levels as determined by RT-qPCR (Figure 3C) and an increase in the repressive histone modification H3K9me2 (Figure S4A), recapitulating repression of gene expression seen in FRDA cells. This smaller reduction in the RNA levels in FXN-GAA-Luc cells compared to FRDA lymphoblastoid cells can be explained by low number of repeats (only ∼310) on the integrated FXN copy.
Next we investigated if R-loops are formed on expanded repeats of the integrated FXN copy in HEK293 cells using DIP analysis (Figure 3D). Similar to patient-derived FRDA lymphoblast cells, we observed 2–3-fold increase in the level of R-loops over expanded repeats region (amplicons B, C and D) in FXN-GAA-Luc cells. This suggests that R-loops are formed on transcribed expanded GAA repeats, independently of their genomic location. To confirm the specificity of this R-loop signal, we employed RNAi to knock down endogenous RNase H1 enzyme, which specifically degrades the RNA in RNA/DNA hybrids [24]. Following depletion of RNase H1 in HEK293 cells, we observed a significant increase in the R-loop signal, suggesting that endogenous RNase H1 can degrade R-loops formed over FXN gene in vivo (Figure 3E, F).
Next we wanted to test if RNase H1 over-expression can resolve R-loops formed on expanded GAA repeats. To this end we over-expressed Flag and RNase H1-Flag constructs in FXN-Luc and FXN-GAA-Luc cells. High level of RNase H1 over-expression was confirmed by RT-qPCR (Figure S4B) and western blot analysis (Figure 3G). Interestingly, following RNase H1 over-expression, we observed a reduction of R-loop signal over the GAA expansion (Figure 3H). In line with these observations, RNase H1 over-expression resulted in up-regulation of FXN transcription from the expanded allele (Figure S4C). This suggests that R-loops formed over expanded repeats in vivo can be resolved by over-expressed RNase H1 and removal of R-loops leads to increase in FXN gene expression.
R-loops trigger FXN transcriptional repression in vivo
Previous studies have demonstrated that expanded FXN GAA repeats are associated with increased levels of heterochromatin marks [4], [6], [7]. To investigate the relationship between R-loops and repressive heterochromatin marks formed on the expanded FXN allele, we employed histone methyltransferase inhibitor BIX-01294, previously shown to reduce the level of H3K9me2 over repeat regions [21]. Following BIX-01294 treatment, we observed a significant reduction in the levels of H3K9me2 chromatin mark (Figure 4A), similar to previous reports [21]. Significantly, following BIX-01294 treatment the level of R-loops over the expanded repeat region remained unchanged (Figure 4B). Similarly, reduction in H3K9me2 chromatin mark had no effect on FXN nascent RNA level (Figure 4C). These data suggest that H3K9me2 chromatin modification is not directly responsible for the FXN transcriptional repression and is likely to be a consequence of the reduced transcription or caused by R-loop formation.
To investigate the ability of R-loops to directly trigger FXN transcriptional repression, we took advantage of camptothecin (CPT), a specific inhibitor of DNA Topoisomerase I (Top1), an enzyme which relieves transcription-induced DNA supercoiling. Loss of Top1 enhances R-loop formation in yeast and human cells [25], [26]. Following CPT treatment, we observed an increase in R-loop formation over the expanded repeat region in FRDA cells while R-loop levels in FXN regions E–G remained unchanged (Figure 5A). This effect was consistent between different patient-derived cell lines (Figure S5A). We detected no effect on R-loop levels in control cells, demonstrating the specificity of CPT treatment to expanded repeats (Figure 5A and S5A). The ability of CPT to increase R-loop levels was not due to CPT-induced covalent links between Top1 and DNA, since Top1 knock-down also resulted in R-loop accumulation (Figure 5E, F).
Interestingly, increase in the level of R-loops coincided with increase in the amount of repressive H3K9me2 mark over FXN regions B–D surrounding the expansion in FRDA cells (Figure 5B and S5B). This also resulted in down-regulation of nascent FXN RNA in FRDA cells, but not in control cells, as observed in three independent control and FRDA cell lines (Figure 5C and S5C–D). These data suggest that R-loops at expanded repeats directly trigger FXN transcriptional repression and promote formation of repressive H3K9me2 marks. To understand the molecular mechanism of this process, we investigated the binding of G9a histone methyltransferase, which deposits H3K9me2 marks on histones, to FXN gene. Interestingly, we observed that G9a is enriched over the expanded region in FRDA cells (Figure 5D). This suggests that R-loops may recruit G9a to the expanded repeat regions, thereby promoting the formation of repressive H3K9me2 marks.
R-loops are formed over (CGG)n expanded repeats of FMR1 gene
To test if R-loop formation is a general feature of trinucleotide expansion diseases, we also examined the FMR1 gene. In Fragile X syndrome patients, the FMR1 allele containing a (CGG)n>200 expansion in the 5′UTR is fully methylated and transcriptionally silenced [27]. Therefore to investigate the potential role of R-loops in FXS, FMR1 transcription was reactivated by treatment with the DNA methylation inhibitor 5-aza-2′-deoxycytidine (5-azadC). This resulted in expression of FMR1 mRNA in FXS cells to 25% of control cells, as previously reported [28]. However, FMR1 expression was unchanged in control cells (Figure 6B). Using DIP, we detected low R-loop signal in control and untreated FXS cells (Figure 6C). Following 5-azadC treatment, we observed ∼4-fold increase in R-loops over the exon 1 region upstream of the expansion in FXS cells, while no significant changes were detected in control cells. The specificity of the DIP signal was confirmed by RNase H treatment (Figure 6D). These data suggest that R-loops are transcriptionally-dependent and localise to the expanded (CGG) repeat region. Since inhibition of DNA methylation only partially reactivates expanded FMR1 allele in FXS cells, it is possible that R-loops at expanded (CGG) repeats prevent full reactivation.
To further characterize the relationship between R-loops and FMR1 expression, we carried out kinetic experiments. In particular, we studied R-loop and FMR1 mRNA levels during the process of transcriptional re-activation with 5-azadC treatment (7 days) followed by 5-azadC wash out with drug-free media for 28 days (Figure 6E, F). We observed that the R-loop levels over the exon 1 of FMR1 gene stayed at the background during activation and wash-out period in control cells (dotted line in Figure 6E). In FXS cells, the R-loops were at their peak during the re-activation procedure with 5-azadC on day 7. After removal of 5-azadC, R-loop levels gradually diminished and completely disappeared after 7 days (day 14 of the full experiment). This pattern of R-loop dynamics correlated with FMR1 expression profile (Figure 6F), suggesting that R-loops are associated with FMR1 gene regulation.
Discussion
We demonstrate that R-loops are formed over endogenous expanded (GAA) and (CGG) repeats in vivo, associated with FRDA and FXS disorders, respectively (Figure 1, 6). We show that these R-loops interfere with nascent Pol II transcription on FXN gene (Figure 2E). We also demonstrate that R-loops can trigger gene silencing irrespectively of their genomic location (Figure 3). R-loops over expanded repeats are very stable in human cells (Figure 2B), possibly due to failure of their complete turn-over by endogenous enzymes, which may contribute to FRDA pathology. Interestingly, expansion-associated R-loops can be resolved by over-expressed exogenous RNase H1, which leads to transcription up-regulation of FXN expression in vivo (Figure 3).
Previous work has demonstrated that co-transcriptionally formed RNA/DNA hybrids mediate transcription elongation impairment in vitro and in yeast S.cerevisiae [29], [30], suggesting that R-loops may provide roadblocks for RNA polymerases. R-loops over expanded repeats may form a structural block, directly interfering with Pol II transcription elongation. Similar to R-loops at the 3′ends of human genes [16], expansion-associated R-loops could promote RNA Pol II termination, resulting in reduction of active Pol II downstream of the expansion, as detected in this study.
Recently it was suggested that repressive chromatin H3K9me2 modification was not directly responsible for the FXN transcriptional repression [21]. In line with this, reversal of repressive DNA methylation on FMR1 gene was not sufficient to fully restore FMR1 expression [28]. We now show that a decrease in the level of the repressive H3K9me2 chromatin mark does not result in decrease of R-loops on the expanded allele or up-regulation of FXN RNA (Figure 4). These data indicate that R-loop formation is an early event in the process of FXN transcriptional gene silencing, which happens prior to the appearance of heterochromatin marks. We also show that increasing R-loop levels lead to an increase in repressive chromatin marks and subsequent repression of FXN gene expression (Figure 5). Furthermore, we observed that recruitment of G9a methyltransferase is enhanced on expanded FXN allele (Figure 5), providing an interesting possibility that R-loops may directly recruit this enzyme to promote H3K9me2 histone mark deposition. Altogether our results suggest that R-loops act as the primary trigger for repression of expanded FXN and FMR1 alleles which may in turn act to promote heterochromatin formation. Consistent with our data, recently it has been demonstrated that promoter-bound trinucleotide repeat-containing mRNA induces epigenetic silencing in Fragile X syndrome [31]. Indeed R-loops have been implicated in the formation and maintenance of heterochromatin at centromeres in S. pombe [32]. In line with this, H3K9me2 modification is also enriched at the R-loop-containing pause region of β-actin gene (Figure S6), essential for Pol II transcriptional termination [16]. This suggests that R-loops may promote H3K9me2 modification at the 3′end of this gene, similar to expanded repeats of FXN gene. We propose that R-loops may be a common feature of many trinucleotide expansion diseases, contributing to their pathology in vivo (Figure 7). The ability of R-loops to trigger transcriptional silencing in trinucleotide expansion diseases makes them an attractive target for future therapeutic approaches to treat these devastating diseases.
In addition to uncovering the molecular mechanisms underlying FRDA and Fragile X pathologies, our work also provides interesting implications for R-loop biology. Taken into consideration this work and the work of others in the field, depending on their genomic location, R-loops may have different functions (reviewed in [11]). Therefore, stable R-loops formed over expanded triplet repeats (this study) may be different from R-loops at the 3′-ends of genes [16], [33] and R-loops formed over CpG island promoters [15], [33]. At promoters, R-loops play a protective role against epigenetic silencing. By contrast, R-loops over FXN expanded repeats correlate with a reduction in transcription elongation and the enrichment of repressive chromatin marks. This suggests that R-loops may be ‘sensed’ differently, depending on their genomic location and sequence composition. Understanding the molecular mechanisms that cells use to distinguish ‘harmful’ from ‘useful’ R-loops is an important biological question in the study of human diseases.
Materials and Methods
Cell culture and drug treatments
Epstein-Barr virus (EBV)-transformed lymphoblastoid cell lines from healthy (GM15851, GM14926, GM06895), FRDA (GM15850, GM16243, GM16209) and FXS (GM03200) patients were obtained from Coriell Institute for Medical Research. Frataxin (GAA) repeat sizes were 650/1030 (GM15850), 800/800 (GM16209) and 670/1170 (GM16243). (CGG) repeat size in FMR1 gene was 530 (GM03200). All experiments were performed with early passages of the cell lines. Lymphoblastoid cell lines were grown in RPMI 1640 medium supplemented with 15% fetal bovine serum (FBS), 100 U/ml penicillin and 100 µg/ml streptomycin at 37°C in 5% CO2. 1 µM 5-azadC (Sigma, A3656) was added to the media for 7 days. 4 µM BIX-01294 (Sigma, B9311) was added to the media for the total of 72 hours. The media was replenished every 24 hours by replacement of half of the conditioned media with fresh media and drug. 10 µM camptothecin (Sigma, C9911) was added for 6 hours. Actinomycin D (Sigma, A9415) was added to a final concentration of 5 µg/ml to the media for 6–48 hours. FXN-Luc and FXN-GAA-Luc HEK293 cells were described in [23] and cultured in DMEM medium supplemented with 10% FBS, 100 U/ml penicillin/streptomycin, 100 µg/ml hygromycin B (Life Technologies). For 5-azadC wash-out experiments, cells were treated with 1 µM 5-azadC for 7 days. On day 7, cell were washed twice with fresh RPMI 1640 medium and cultured in the absence of 5-azadC during the indicated time.
ChIP analysis
ChIP analysis on endogenous genes was carried out as previously described [34], [35]. 5 µg of the following antibodies were used: Pol II antibody (Santa Cruz, H-224), H3 (AbCam, ab1791), H3K9me2 (AbCam, ab1220), G9a (AbCam, ab40542). The immuno-precipitated DNA was used as template for real-time quantitative PCR performed using a Rotor-Gene RG-3000 machine (Corbett Research). The PCR mixture contained QuantiTect SYBR green PCR master mix (Qiagen), 2 µl of the template DNA and corresponding primers from Table S1. Cycling parameters were 95°C for 15 min, followed by 45 cycles of 94°C for 20 s, 58°–62°C for 20 s, and 72°C for 20 s. Fluorescence intensities were plotted against the number of cycles by using an algorithm provided by the manufacturer. Amount of immuno-precipitated protein at a particular gene region was calculated as ‘% of Input’ after subtracting the background signal, as determined by the ‘no antibody’ control.
Expansion PCR in HEK293 cells
20 ng of genomic DNA was used as template in a 25 µl PCR reaction, containing 2.5 U Biotaq DNA polymerase, 3 mM MgCl2, 0.4 mM dNTPs, 0.4 µM GAA104F primer, 0.4 µM GAA629R primer in 1× NH4 reaction buffer. Products were amplified using protocol from [36] with minor modifications. In particular, 5 min at 94°C were followed by 10 cycles of 94°C for 20 s, 65°C for 30 s, 72°C for 5 min. This was followed by 20 cycles of 94°C for 20 s, 65°C for 30 s and 72°C for 5 min, with the 72°C step becoming 20 s longer in each cycle. After a final step at 72°C for 10 min, PCR products were resolved on a 1% agarose gel.
DIP analysis
DNA immuno-precipitation (DIP) analysis on endogenous genes was performed with antibody, recognising RNA/DNA hybrids, purified from S9.6 hybridoma cell lines [37], as described in [16]. In particular, lymphoblastoid and HEK293 cells were split one day before DIP. 10×106 cells were harvested, washed in PBS and incubated in cell lysis buffer (85 mM KCl, 5 mM PIPES pH 8.0, 0.5% NP-40) for 10 min on ice. Nuclei were collected by centrifugation and then incubated in nuclei lysis buffer (50 mM TRIS pH 8.0, 5 mM EDTA, 1% SDS) on ice. Proteins were digested by incubation with proteinase K (Roche) for 3 h at 55°C. Proteins and cell debris were removed by centrifugation after addition of KOAc to the final concentration of 1 M. Genomic DNA containing R-loops was then precipitated by addition of isopropanol. After washing the DNA pellet with 70% EtOH, genomic DNA was resuspended in 400 µl IP dilution buffer (16.7 mM TRIS pH 8.0, 1.2 mM EDTA, 167 mM NaCl, 0.01% SDS, 1.1% Triton X-100) and used for sonication (Diagenode Bioruptor). Bioruptor (Diagenode) settings were 3 min sonication, at the Medium setting 30 sec on/30 sec off interval and the average size of the fragments was ∼500 nt. Sonicated genomic DNA was then pre-cleared with 50 µl protein A agarose beads (Millipore) in 3 ml IP dilution buffer including protease inhibitors (0.5 mM PMSF, 0.8 µg/ml pepstatin A, 1 µg/ml leupeptin) for 1 h at 4°C. 10 µl of S9.6 antibody was added to DNA corresponding to 10×106 cells. Immuno-precipitation was carried out over night at 4°C. Subsequent washes and elution steps are identical to the procedure as described for ChIP.
The immuno-precipitated, non-precipitated, and input DNAs were used as templates for qPCR. DIP RNase H-sensitivity analysis was carried out following the genomic isolation and prior to immuno-precipitation step with the addition of 25 U of RNase H (NEB, M0297S). 100 µl nuclease digestion reaction contained 1× reaction buffer, and it was performed for 6 hours at 37°C. Amount of immuno-precipitated RNA/DNA hybrid at a particular gene region was calculated as ‘% of Input’ after subtracting the background signal, as determined by the ‘no antibody’ control. In the case of FXN gene, all the values are relative to the FXN B amplicon in control cells.
RNA analysis
Total RNA was harvested using TRIZOL reagent (Invitrogen) followed by DNase I treatment (Roche). 1–2 µg of total RNA was reverse-transcribed using SuperScript Reverse Transcriptase III (Invitrogen) with random hexamers (Invitrogen), oligodT primer (for polyA+ RNA) or gene-specific reverse primer (Table S1). The qPCR primers for amplification of polyA+ RNAs were the following: β-actin (ex5F/ex6R), GAPDH (F/R3), γ-actin (γ-actin spliced F/R), FXN (ex3F/ex4R), FMR1 (ex14 F/ex 15R). For analysis of nascent RNA in Figures 2A and 4C FXN primer FXN D was used, while in Figure 3C FXN primer B was used. For quantitative real-time PCR, 2 µl of cDNA was analyzed using a Rotor-Gene RG-3000 real-time PCR machine (Corbett Research) with QuantiTect SYBR green (Qiagen). For analysis of nascent FXN-Luc RNA in HEK293 cells, lucR primer was used for reverse transcription, and in4F and ex5R were used for qPCR.
RNAi and protein analysis
The RNAi was carried out as described [38]. Control siRNA duplex was 5′-UAGCGACUAAACACAUCAA -3′ (Thermo Scientific siGENOME Non-Targeting siRNA #1D-001210-01-20), RNase H1 siRNA duplex was s48357 (Ambion). mRNA target sequence for Topoisomerase I siRNA duplex was 5′-GGACUCCAUCAGAUACUAU -3′. Total protein extracts were harvested using RIPA buffer. 20 and 40 µg of total protein extracts were resolved on SDS-PAGE and detected by Western blotting. Western blots were probed with Topoisomerase I (AbCam, ab109374), actin (Sigma, A2066), RNase H1 (AbCam, ab83179) antibodies.
RNase H1 over-expression
FXN-Luc and FXN-GAA-Luc cells were freshly split into 10 cm dishes and transfected on the following day with 10 µg Flag or RNase H1-Flag plasmids using TransFectin reagent (BioRad), following the manufacturer's instructions. Cells were harvested 48 hours after transfection. RNaseH1-Flag plasmid was cloned by replacing the GFP tag in the RNaseH1-GFP plasmid, provided by Prof.R.J. Crouch, with the Flag tag using RNaseH1-FLAG(F) and RNaseH1-FLAG(R) primers.
Br-UTP nuclear run-on analysis
The Br-UTP NRO analysis was carried as described in [16]. The equivalent of 8×106 nuclei from lymphoblastoid cells were used for each Br-UTP NRO reaction.
Statistical analysis
Unless otherwise stated, the figures present the average values of at least three independent experiments +/− SEM. Asterisks (*) indicate statistical significance (* p<0.05; ** p<0.01; *** p<0.001), based on unpaired, two-tailed distribution Student's t test.
Supporting Information
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Štítky
Genetika Reprodukčná medicínaČlánok vyšiel v časopise
PLOS Genetics
2014 Číslo 5
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