A Truncated NLR Protein, TIR-NBS2, Is Required for Activated Defense Responses in the Mutant

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Secretory pathways play an important role in the plant immune response by delivering antimicrobial compounds and metabolites to the site of infection. The evolutionarily conserved exocyst complex is involved in exocytosis, the final step in the secretory pathway. We showed that loss of the function of EXO70B1, a subunit of exocyst complex, results in activated defense responses, and enhanced resistance to a range of pathogens. We found that EXO70B1 associates with the SNARE complex protein SNAP33, which is involved in focal secretion of defense-related proteins. Enhanced disease resistance and cell death in the exo70B1 mutant are dependent on TIR-NBS2 (TN2), an atypical intracellular immune receptor-like protein that lacks leucine-rich repeats. TN2 physically associates with EXO70B1, and TN2 transcripts accumulate at much higher levels in the exo70B1 mutant. These data are consistent with a model where activation of a receptor pathway containing TIR-NBS2 is responsible for activated defense responses and cell death in exo70B1. Our data further suggest that this, and possibly other, exocyst components could be targets of effectors that are guarded by immune receptors.

Vyšlo v časopise: A Truncated NLR Protein, TIR-NBS2, Is Required for Activated Defense Responses in the Mutant. PLoS Genet 11(1): e32767. doi:10.1371/journal.pgen.1004945
Kategorie: Research Article
prolekare.web.journal.doi_sk: https://doi.org/10.1371/journal.pgen.1004945

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