Asymmetric Transcript Discovery by RNA-seq in . Blastomeres Identifies , a Gene Important for Anterior Morphogenesis

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At key moments in development, asymmetric cell divisions give rise to daughter cells of differing characteristics, a process that promotes cell-type diversity in complex organisms. The first cell division of the C. elegans early embryo is a powerful model for understanding asymmetric cell division because the timing of divisions and the placement of their division planes are precise and reproducible. We surveyed the mRNA content of each daughter cell in the C. elegans 2-cell embryo using low-input RNA sequencing. We identified several hundred asymmetric transcripts and tested them for functions in development. We found that the gene neg-1 produced mRNA and protein preferentially on the anterior (head-side) of 2-cell and 4-cell stage embryos and that loss of neg-1 led to consequences in anterior morphogenesis later in development. We also analyzed the asymmetric transcripts using quantitative microscopy, bioinformatics comparisons with previously existing datasets, and RNA sequence motif discovery to gain insight to the mechanisms by which asymmetric abundance patterns arise.

Vyšlo v časopise: Asymmetric Transcript Discovery by RNA-seq in . Blastomeres Identifies , a Gene Important for Anterior Morphogenesis. PLoS Genet 11(4): e32767. doi:10.1371/journal.pgen.1005117
Kategorie: Research Article
prolekare.web.journal.doi_sk: https://doi.org/10.1371/journal.pgen.1005117

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