Dual-Specificity Anti-sigma Factor Reinforces Control of Cell-Type Specific Gene Expression in

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Precise temporal and cell-type specific regulation of gene expression is required for development of differentiated cells even in simple organisms. Endospore development by the bacterium Bacillus subtilis involves only two types of differentiated cells, a forespore that develops into the endospore, and a mother cell that nurtures the developing endospore. During development temporal and cell-type specific regulation of gene expression is controlled by transcription factors called sigma factors (σ). An anti-sigma factor known as CsfB binds to σG to prevent its premature activity in the forespore. We found that CsfB is also expressed in the mother cell where it blocks ectopic activity of σG, and blocks the activity σE to allow σK to take over control of gene expression during the final stages of development. Our finding that CsfB directly blocks σE activity also explains how CsfB plays a role in preventing ectopic activity of σE in the forespore. Remarkably, each of the major roles of CsfB, (i.e., control of ectopic σG and σE activities, and the temporal limitation of σE activity) is also accomplished by redundant regulatory processes. This redundancy reinforces control of key regulatory steps to insure reliability and stability of the developmental process.

Vyšlo v časopise: Dual-Specificity Anti-sigma Factor Reinforces Control of Cell-Type Specific Gene Expression in. PLoS Genet 11(4): e32767. doi:10.1371/journal.pgen.1005104
Kategorie: Research Article
prolekare.web.journal.doi_sk: https://doi.org/10.1371/journal.pgen.1005104

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