Synergistic and Independent Actions of Multiple Terminal Nucleotidyl Transferases in the 3’ Tailing of Small RNAs in Arabidopsis

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Small silencing RNAs are key regulators of gene expression in both plants and animals. HEN1-mediated 3’ terminal 2’-O-methylation plays a crucial role in small RNA stability control. In the absence of HEN1, several types of small RNAs become frequently uridylated (non-templated uridine addition) and trimmed, a phenomenon that is conserved across species. However, the underlying molecular mechanism is barely understood. In this study, we have discovered UTP: RNA uridylyltransferase (URT1) that acts synergistically with HESO1 in miRNA uridylation, in addition to its role in oligo-adenylated mRNA uridylation. Analyzing the miRNA profiles also reveals the existence of multiple terminal nucleotidyl transferases in the miRNA tailing process and an antagonistic action between uridylation and trimming. We believe this study will shed light on our understanding of how various terminal nucleotidyl transferases recognize their substrates and function coordinately.

Vyšlo v časopise: Synergistic and Independent Actions of Multiple Terminal Nucleotidyl Transferases in the 3’ Tailing of Small RNAs in Arabidopsis. PLoS Genet 11(4): e32767. doi:10.1371/journal.pgen.1005091
Kategorie: Research Article
prolekare.web.journal.doi_sk: https://doi.org/10.1371/journal.pgen.1005091

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