C-di-GMP Regulates Motile to Sessile Transition by Modulating MshA Pili Biogenesis and Near-Surface Motility Behavior in

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The human pathogen Vibrio cholerae causes the debilitating disease cholera through ingestion of contaminated food and water. V. cholerae is a natural inhabitant of aquatic environments. Transmission of V. cholerae to the human host is dependent on survival of the pathogen in aquatic reservoirs where it is challenged with many stressors, including changes in the physiochemical parameters of environments and predation by protozoa and phages. One method utilized to endure these assaults is to form a multicellular community called a biofilm. The signaling molecule cyclic dimeric guanosine monophosphate (c-di-GMP) is utilized to induce biofilm formation in many bacteria, including V. cholerae. We demonstrate that c-di-GMP promotes the production of a cell surface structure called MshA pili by binding the molecular motor responsible for polymerizing pilus subunits. These pili are adhesive appendages that are essential for attachment to surfaces. This study identifies a novel mechanism for c-di-GMP regulation of pilus production through interactions with the molecular motor responsible for pilus assembly. Since many bacteria utilize pili for attachment to surfaces and c-di-GMP as a pro-biofilm signaling molecule, the mechanism for pilus regulation and biofilm formation described here may be widespread among many pathogens.

Vyšlo v časopise: C-di-GMP Regulates Motile to Sessile Transition by Modulating MshA Pili Biogenesis and Near-Surface Motility Behavior in. PLoS Pathog 11(10): e32767. doi:10.1371/journal.ppat.1005068
Kategorie: Research Article
prolekare.web.journal.doi_sk: https://doi.org/10.1371/journal.ppat.1005068

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