Comparative Life Cycle Transcriptomics Revises Genome Annotation and Links a Chromosome Duplication with Parasitism of Vertebrates

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Leishmania are single-celled parasites that are transmitted between animal hosts by the bite of sand flies. Once inside their animal hosts they abandon their extracellular habit and invade cells of the immune system, called macrophages. This oscillation between hosts requires the parasite to be able to adapt to dramatically different environments. To help unravel the multitude of biochemical, ultrastructural and lifestyle differences that distinguish these specialised life cycle stages we characterised and contrasted the transcriptomes of insect and mammalian adapted forms. Using bioinformatic approaches we revised the genome annotation and discovered nearly 1,000 new genes that had not been described before. We found that over 3,000 genes change in their expression to facilitate the change in host environment including those involved in specifying cell shape, extracellular appearance and biochemistry. Furthermore we reveal that an ancient chromosome duplication shared by all Leishmania species may have contributed to the adaptation of these globally important parasites to parasitism of vertebrates.

Vyšlo v časopise: Comparative Life Cycle Transcriptomics Revises Genome Annotation and Links a Chromosome Duplication with Parasitism of Vertebrates. PLoS Pathog 11(10): e32767. doi:10.1371/journal.ppat.1005186
Kategorie: Research Article
prolekare.web.journal.doi_sk: https://doi.org/10.1371/journal.ppat.1005186

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