Identification of a Novel Lipoprotein Regulator of Spore Germination

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Clostridium difficile is a spore-forming bacterium capable of causing severe diarrhea. The dormant spore-form of C. difficile is necessary to cause infection, since vegetative cells of this organism cannot survive in the presence of oxygen. Spores are difficult to eradicate because they can withstand extreme environmental conditions and chemical insults including antibiotics. However, since spores cannot grow, they must transform back into actively replicating cells once the appropriate environmental conditions are sensed through a process called germination. A key step during germination is the break-down of a specialized cell wall layer in the spore known as cortex by the SleC hydrolase. In this paper, we identify GerS as a novel lipid-modified protein that is important for C. difficile germination to occur. GerS is made at high levels during spore formation and gets packaged into mature spores. We show that GerS is required for the cortex hydrolase SleC to degrade the protective cortex layer, since a strain lacking GerS does not lose its cortex layer. Loss of GerS prevents C. difficile from causing infection in a hamster model of infection, suggesting that GerS is a novel target for drug development.

Vyšlo v časopise: Identification of a Novel Lipoprotein Regulator of Spore Germination. PLoS Pathog 11(10): e32767. doi:10.1371/journal.ppat.1005239
Kategorie: Research Article
prolekare.web.journal.doi_sk: https://doi.org/10.1371/journal.ppat.1005239

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