Crosslinking of a Peritrophic Matrix Protein Protects Gut Epithelia from Bacterial Exotoxins

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Intestinal homeostasis is ensured by a subtle balance between bacteria and host immunity. Gut epithelial barriers, such as the mucus layer in mammals and the peritrophic matrix in invertebrates, have a protective function for the host, as they are impermeable to invading intestinal microbes. Here we found that, in the fly Drosophila melanogaster, transglutaminase (TG), a molecular glue involved in protein-protein covalent bond formation, is essential for peritrophic matrix formation by converting the peritrophic protein drosocrystallin into a stable fiber-like structure and inhibition of pathogenic bacteria. Knockdown of the TG gene led to increased permeability of the peritrophic matrix and greatly increased the susceptibility to a toxic bacterial protease. TG contributes to form a stable fiber-like barrier on the peritrophic matrix and increase tolerance to pathogenic microorganisms.

Vyšlo v časopise: Crosslinking of a Peritrophic Matrix Protein Protects Gut Epithelia from Bacterial Exotoxins. PLoS Pathog 11(10): e32767. doi:10.1371/journal.ppat.1005244
Kategorie: Research Article
prolekare.web.journal.doi_sk: https://doi.org/10.1371/journal.ppat.1005244

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