Rapidly Evolving Genes Are Key Players in Host Specialization and Virulence of the Fungal Wheat Pathogen ()

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Zymoseptoria spp provides a unique model system to study the underlying genetics of host specialization of plant pathogens. Closely related Zymoseptoria species, including the prominent wheat pathogen Z. tritici, have recently specialized to distinct grass hosts. Positively selected substitutions have played a central role in the acquisition of new host specificities. We have identified a small set of genes showing signatures of positive selection. We demonstrate that three of these four candidate genes play an important role during host infection. Two mutants of Z. tritici were impaired in virulence; a third mutant showed a hypervirulent phenotype. New protein specificities not only include changes at the amino acid sequence level but also at the level of the protein structure. We conducted a gene replacement experiment to test if mutant phenotypes in Z. tritici could be complemented by the insertion of orthologous genes from the two closely related species Z. pseudotritici and Z. ardabiliae. For two genes, we confirm that the species-specific protein changes are essential for proper protein functioning in Z. tritici; key traits involved in the evolution of virulence and host specificity of this prominent pathogen can be characterized via a combination of evolutionary predictions and functional analyses.

Vyšlo v časopise: Rapidly Evolving Genes Are Key Players in Host Specialization and Virulence of the Fungal Wheat Pathogen (). PLoS Pathog 11(7): e32767. doi:10.1371/journal.ppat.1005055
Kategorie: Research Article
prolekare.web.journal.doi_sk: https://doi.org/10.1371/journal.ppat.1005055

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