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Vaccination Drives Changes in Metabolic and Virulence Profiles of


The bacterium Streptococcus pneumoniae is a major cause of life-threatening pneumonia, septicaemia and meningitis worldwide. Pneumococci are covered by a polysaccharide capsule of which there are over 90 distinct serotypes. Available vaccines target a small subset (either 7, 10 or 13) of these capsular serotypes but, following their introduction, increases in the relative amount of disease caused by non-vaccine serotypes have been observed in several countries. Here we offer an alternative explanation for this phenomenon to the traditional concept of Vaccine-Induced-Strain-Replacement whereby the removal of interference from vaccine strains allows non-vaccine strains to fill the niches left vacant by them. We show, instead, that vaccination induces genotypic changes among non-vaccine strains which can lead to an increase in both transmissibility and virulence. Using a mathematical model of genomic evolution, in which strains are split into antigenic, metabolic and virulence-associated components, we show that metabolic and virulence-associated components originally associated with vaccine serotypes become associated with non-vaccine serotypes following vaccination. We term this Vaccine-Induced-Metabolic-Shift and propose that it explains post-vaccine changes observed in pneumococcal population structure in a number of locations worldwide.


Published in the journal: Vaccination Drives Changes in Metabolic and Virulence Profiles of. PLoS Pathog 11(7): e32767. doi:10.1371/journal.ppat.1005034
Category: Research Article
doi: https://doi.org/10.1371/journal.ppat.1005034

Summary

The bacterium Streptococcus pneumoniae is a major cause of life-threatening pneumonia, septicaemia and meningitis worldwide. Pneumococci are covered by a polysaccharide capsule of which there are over 90 distinct serotypes. Available vaccines target a small subset (either 7, 10 or 13) of these capsular serotypes but, following their introduction, increases in the relative amount of disease caused by non-vaccine serotypes have been observed in several countries. Here we offer an alternative explanation for this phenomenon to the traditional concept of Vaccine-Induced-Strain-Replacement whereby the removal of interference from vaccine strains allows non-vaccine strains to fill the niches left vacant by them. We show, instead, that vaccination induces genotypic changes among non-vaccine strains which can lead to an increase in both transmissibility and virulence. Using a mathematical model of genomic evolution, in which strains are split into antigenic, metabolic and virulence-associated components, we show that metabolic and virulence-associated components originally associated with vaccine serotypes become associated with non-vaccine serotypes following vaccination. We term this Vaccine-Induced-Metabolic-Shift and propose that it explains post-vaccine changes observed in pneumococcal population structure in a number of locations worldwide.

Introduction

Pneumococci can be stratified into over 90 different “serotypes” according to the antigenic properties of their polysaccharide capsule; only a small number (~10) of these, however, are responsible for most cases of invasive disease worldwide [1]. Pneumococcal populations are also highly diverse in non-antigenic genes, and are commonly classified into sequence types (ST) by Multi Locus Sequence Typing (MLST) of seven metabolic housekeeping genes [2]. Given the high rates of recombination observed in the pneumococcus [3], it might be expected that most serotypes would be linked to a variety of STs, yet many studies (eg [4]) show an intriguing pattern of largely non-overlapping associations between capsular serotype and ST in pneumococcal populations (Table A in S1 Text and Fig 1A). These associations are not stable: capsular switching events (whereby an ST acquires a different capsular serotype) have been documented to occur regularly throughout the past 7 decades [5], and since the introduction of the heptavalent PCV7 vaccine (first licensed in the USA in 2000), it has been noted that many STs that were previously associated with vaccine serotypes now occur in association with non-vaccine serotypes. For example, ST320 (previously associated with the vaccine serotype 19F [6]) has replaced ST199 as the most common MLST type associated with non-vaccine serotype 19A in the US [710] (Fig 1B). Increases have also occurred in the prevalence of the ST69519A strain in which the vaccine serotype 4 capsule has been switched for a 19A capsule [11] (Fig 1B). Similarly, in Korea, where PCV7 caused a drop in vaccine serotypes 23F and 19F (but notably not 6B), ST81 (previously associated with serotypes 23F and 19F) is now the primary MLST type of serotype 6A [12]. Several theoretical models have demonstrated the potential of strain-targeted vaccines to increase the prevalence of non-vaccine serotypes due to the removal of cross-immunity or direct resource competition [1319], but these do not explain why changes have occurred in the MLST composition of non-vaccine serotypes.

#obr:1#

We have previously proposed that a combination of immune-mediated interference between identical antigenic types and direct competition between identical metabolic types can generate non-overlapping associations between antigenic types and STs in populations of the bacterial pathogen Neisseria meningitidis [20, 21]. In this paper, we explore whether pneumococcal population structure, and the changes that have occurred since vaccination, can be explained within a similar conceptual framework (Table 1) in which pathogen strains are profiled by antigenic type (AT), metabolic type (MT) and additional non-capsular virulence factors (VF). Strains sharing the same AT experience immunological interference as a consequence of type-specific immunity; interference between strains sharing the same MT or VF may occur through direct competition for similar substrates or for binding to specific host receptors or selected components of the extracellular matrix. Using this framework, we outline the conditions under which associations may arise between antigenic, metabolic and virulence properties of strains within pneumococcal populations and predict how these may shift under vaccination.

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Results

Pneumococcal populations contain discrete metabolic types which associate specifically with capsular serotypes

The theoretical framework shown in Table 1 can be applied to a wide range of pathogens, but within the context of S. pneumoniae, the capsular serotype is the principal determinant of AT. To determine whether pneumococcal populations can also be considered to contain discrete MTs, we interrogated 616 whole pneumococcal genomes published by Croucher et al. [22] for allelic differences among 876 metabolic/uptake loci. Associations between alleles of metabolic loci across the genome were found to be highly non-random (Fig 1C), and to exhibit significantly higher levels of linkage disequilibrium (LD) than randomly selected genes not associated with metabolic/transport processes (Fig 1D and 1E). This suggests that a “metabolic profile” may comprise a set of co-evolved genes, which have synergistically adapted to exploit a particular metabolic niche [23], and that such profiles may be stably maintained because any deviation constitutes a loss of fitness [21]. It is possibly that non-metabolic genes contain a higher share of mobile genetic elements but, other than this, we know of no other explanation which could account for the higher levels of association observed between metabolic loci than non-metabolic loci.

We also found the metabolic profiles of isolates belonging to the same ST to be highly concordant (Table F in S1 Text and S1B and S1C Fig). Although a small number of STs shared highly similar metabolic profiles, they also tended to be closely related according to MLST-typing: for example, ST236, ST320 and ST271 manifest highly similar metabolic alleles, and belong to the same MLST-defined clonal complex (CC271). We thus conclude that ST, although defined by only 7 metabolic housekeeping genes, serves as a marker for an extended MT.

We found that metabolic profiles were highly consistent within a serotype (Table D in S1 Text and Fig 1C and S1A Fig), and differed significantly between serotypes (Table E in S1 Text). Certain serotypes were associated with more than one metabolic profile (eg. 6A), and one or two profiles were shared between serotypes (eg. 19A and 15B/C), but the overall pattern was strongly non-overlapping.

Vaccine induced metabolic shift

A range of different population structures can arise within our theoretical framework (Table 1) depending on the strength of serotype-specific immunity (γ) and direct resource competition (ψ) between strains sharing identical metabolic types. Under moderate to high levels of γ and ψ, pneumococcal populations may either (i) be dominated by a highly transmissible MT, (ii) contain a range of similarly transmissible MTs which exhibit non-overlapping associations with existing serotypes, or (iii) exist in a transitional state between these two extremes (Fig 2C, S3, S4 and S5 Figs).

#obr:2#

We explored the effects of serotype-specific vaccination within these different scenarios. We observed, under circumstances where serotype and MT exist in non-overlapping combinations (eg. a1 and b2), that vaccination against a particular serotype can lead to the apparent ‘transfer’ of its metabolic types to a non-vaccine serotype (Fig 2A): a phenomenon we term Vaccine-Induced Metabolic Shift (VIMS). This is because vaccination against serotype a (where MT1 has a slight transmission advantage over MT2) results in the expansion of b1 which, despite possessing a higher transmission efficiency than b2, has been hitherto suppressed by competition with a1. This may or may not lead to the exclusion of b2 depending on the strength of serotype-specific immunity and the difference in transmission efficiency between MT1 and MT2. If both b1 and b2 are present before vaccination (in addition to a1), vaccination against serotype a tends to lead to the loss of MT2 from the system: in other words, the removal of a1 allows b1 to competitively exclude b2.

These results can be extended to multiple serotypes and MTs (S4 Fig), and also to additional loci that may, for example, encode extra-capsular and extra-metabolic virulence factors (S5 Fig). We find, as above, that the pneumococcal population becomes structured into non-overlapping combinations of serotype, MT and VF as a consequence of interference between strains that share alleles at any of these loci, provided differences in transmissibility between MTs and VFs are not too high and the costs of virulence are low. We observe, for example (S5 Fig), the persistence of only a small subset (a1+, b2-, c3-) of the 18 possible variants in a freely recombining pneumococcal population where (a, b, c), (1, 2, 3), and (+,-) respectively represent variants of AT, MT and VF. This is because, although b1+ may be intrinsically more transmissible than b2-, the latter has the competitive advantage while a1+ is in circulation because it differs from a1+ at all three loci; for similar reasons, c3- outcompetes c1+, c1-, c2+, c2- and c3+, even though they may all have higher intrinsic transmissibility. This is consistent with the observation that certain serotypes are over-represented in pneumococcal invasive disease [1,4]. Vaccination against the most ‘virulent’ serotype (here a) promotes the expansion of c1+, which had been hitherto suppressed by competition with a1+. This is because (i) it does not share alleles at the metabolic or virulence loci with the already co-circulating strain b2-, and (ii) it outcompetes c3+ (which also does not share alleles with b2-) due to metabolic type 1 having a higher transmission efficiency.

Discussion

Non-overlapping associations between antigenic and metabolic types are commonly observed in both pneumococcal and other pathogenic bacterial populations (Table B in S1 Text) with, typically, only certain of these combinations being associated with invasive disease [1,4]. Here we demonstrate that this structuring may arise within a theoretical framework containing freely recombining antigenic and metabolic components and virulence factors, due to the combined action of immune selection and direct resource competition.

Does the biology of the pneumococcus fulfil the criteria under which these structures arise in our model? There are few data available on naturally-acquired immunity to pneumococcal carriage, but longitudinal studies [2426] indicate that serotype-specific immune responses offer protection (though not complete) against further infection. The genomic analysis presented in this paper endorses the view that pneumococcal populations contain discrete metabolic types representing "fitness peaks" of similar heights within a landscape shaped by frequent genetic transfer [21]. Differences in the transporters and uptake systems encoded in the genome dictate specific substrate repertoires for different strains [27,28], therefore strains with identical MTs are likely to experience strong competition within the nutrient-limiting environment of the host nasopharynx. Pneumococcal strains also vary in the relative intensity at which different substrates are fermented [29, 30] and therefore may also compete if they exhibit identical hierarchies of substrate utilisation/fermentation profiles. Our model indicates that a combination of these selective forces can act to structure the pneumococcal populations into non-overlapping combinations of serotype and MT, provided that differences in transmissibility between MTs are small (Fig 2), although wide differences in serotype/MT combinations can exist due to differences in transmission coefficients associated with serotype (S3 Fig). The structuring observed within our framework will not be maintained under high differences among MTs, since the most transmissible type would dominate instead; under these circumstances, the observed associations between serotype and MT would be better explained under processes such as a neutral micro-epidemic evolution [31] or through epistasis [5], although recent analyses [32] indicate the latter is unlikely.

A strength of our framework is that it can also explain why associations between serotype and MT may be intrinsically unstable [3335] and how vaccination can induce a metabolic shift (VIMS) whereby the MTs of vaccine strains to become associated with non-vaccine serotypes. Our results accord with observations of MLST-defined metabolic types shifting onto non-vaccine strains following the introduction of PCV7 (Fig 1B). Cases of VIMS can also be identified within the whole genome data [22] analysed above. Non-vaccine serotypes 15B/C and 19A can be seen to acquire a new MT sharing at 84.6% (694 out of 820) and 90.0% (737 out of 819) of metabolic loci respectively with vaccine serotype 9V, and an MT with 89.8% (729 out of 812 loci) percentage identity with ST320 in 19F is observed to associate with 19A following vaccination. Positive selection for persistence of metabolic types with new capsules after vaccination also underscores the idea that such metabolic types contain a particularly successful constellation of alleles that allows them to exploit a particular metabolic niche.

It is important to note that these results do not imply that vaccines play a mechanistic role in inducing capsular switching variants; on the contrary, capsular switch variants can be present at low frequencies prior to vaccination but will only expand subsequently due to the removal of competition from vaccine strains. Our model is thus able to resolve why, for example, although the ST69519A vaccine escape variant in the US was first reported in 2003 [11], evolutionary analyses indicate that the capsule switch may have taken place prior to 1997 [22]. Overall, our model predicts that rare genotypes (which may or may not have been there prior to vaccination) may increase in frequency after vaccination due to the removal of ecological competition with vaccine serotypes possessing very similar metabolic profiles.

This transition to a new metabolic profile in non-vaccine serotypes may accompanied by an change in virulence due to a number of reasons: (i) competitive interference from vaccine strains sharing the same virulence factors will have been removed by vaccination (ii) the cost of virulence may be offset by a slight increase in transmissibility due to the acquisition of a different metabolic profile (S4 Fig), and (iii) epistatic interactions between virulence and metabolic types may favour the emergence of virulence on the new metabolic background. A possible example of this phenomenon is the large increase in piliated strains observed in the US since PCV7 vaccination [36, 37]. There are two types of pili in pneumococcus, type I (PI-1) and type II (PI-2), which are found in 30% and 16% of strains respectively [38,39]. In Massachusetts, PI-1 was associated primarily with vaccine-type serotypes before vaccination in 2000. PI-1 subsequently decreased in prevalence with the declining vaccine serotypes, but re-emerged in 2004–2007 in association with non-vaccine serotypes, in particular serotype 19A [36]. Similarly, there has been a 40% increase in PI-2 in serotype 19A following the introduction of PCV7 in Atlanta, Georgia [37]. Significant negative associations have been observed, for example, in co-colonisation of piliated pneumococci and Staphylococcus aureus [24]; it would be reasonable to assume that the same type of competition occurs between strains of piliated pneumococci. We also observe, within the WG data, that a number of alleles at loci which have been implicated in increased virulence or pathogenesis were shared between vaccine strains isolated in 2001, and non-vaccine strains isolated after vaccination, in 2004 and 2007 (Table C in S1 Text).

The outgrowth of non-vaccine variants possessing metabolic and virulence factors previously associated with vaccine strains, as predicted by this model, has important implications for the continued success of strain-targeted vaccination programs. Within our framework, drug-resistance alleles may also shift to non-vaccine serotypes due to the removal of competition at these loci, thereby exacerbating the problem. Indeed, in North America, the majority of penicillin-resistant 19A isolates are linked with MLST types (such as ST320) previously associated with vaccine serotypes [6]. Also, in Italy, the highly prevalent antibiotic resistant ST230 clone, previously associated with vaccine serotypes 14 and 19F, is now predominantly observed with non-vaccine serotypes 19A and 24F [40]. As genomic sequencing becomes a routine part of epidemiological surveillance, our theoretical approach—in which strains can be envisaged as comprising a number of interchangeable modular units specifying antigenic, metabolic, virulence and antibiotic resistance properties—can provide a powerful conceptual framework for the analysis of pathogen population biology and of the genomic impact of vaccination programs.

Materials and Methods

Metabolic analysis

We explored the allelic variation between metabolic loci in a comprehensive sample of 616 genomes published previously by Croucher et al. [22], comprising carriage strains isolated from Massachusetts, USA, in 2001, 2004 and 2007 (see S1 Dataset for the Accession Numbers). Sequence reads were taken from the project ERP000889 on the European Nucleotide Archive (http://www.ebi.ac.uk/) and assembled using an automated pipeline with the Velvet algorithm. Annotation was carried out using the BIGSdb software with an automated BLAST process, and the genomes were analyzed using the Genome Comparator tool (with ATCC 700669 as the reference) [41]. Alleles identical to the Reference Genome were designated as “1”, and subsequent sequences which differ at one or more bases labelled consecutively, and are represented in the S1 Dataset by arbitrary colours with missing alleles shown in black.

We searched through the 2135 identified coding sequences of the reference strain for genes involved in metabolic processes and nutrient uptake. The coding sequences were functionally assigned as “metabolic & transport”, “neither”, or “unknown function” according to the KEGG Orthology (KO) groupings of the KEGG database (Kyoto Encyclopedia of Genes and Genomes; http://www.genome.jp/kegg/). We identified 877 genes involved in metabolic and/or uptake processes.

To explore the structuring of metabolic/uptake alleles among serotypes, we identified the modal allele for each locus and calculated the frequency (or modal percentage identity, MPI) with which it occurred over all the isolates in a given serotype compared to the 10 isolates randomly selected (without replacement) from the total sample (Table D in S1 Text). We also identified a modal metabolic profile (comprising the most frequent alleles found at each locus) for each serotype and the pairwise percentage of alleles which were shared with the modal metabolic profile of other serotypes was calculated (Table E in S1 Text). Similar analyses were performed with respect to ST (S1 Fig).

We used the D’ measure of Linkage Disequilibrium to investigate the strength of associations between pairs of 100 randomly selected metabolic/transport loci across 300 genomes (which were randomly selected for each comparison) compared to 100 randomly selected loci that were not involved in metabolic processes according to the KEGG Orthology categories (www.genome.jp/kegg) (including those, for example, pertaining to genetic information processes such as translation and transcription). D’ was analysed using the 2LD package [42].

Epidemiological model

We first consider the dynamics of a pathogen population containing two antigenic types (i = a,b) and two metabolic types (j = 1,2). We define yij to be the proportion of the population infected by strain ij (e.g. a1), zi is the proportion of the population immune to serotype i, μ is the average host death rate, and σij is the rate of loss of infectiousness associated with strain ij. Let us first assume that infection by a particular strain (e.g. a1) cannot occur among individuals who are immune or infected with the same serotype (ie. za). Let us also assume that infection cannot occur among individuals currently infected by other strains with the same metabolic type. Under these circumstances (see S1 Text), the rate of change in the host population infected with strain a1, ya1, and proportion of hosts immune or infected with the same serotype, za, can be given by:

Equations for other strains follow a similar form with λij = βijyij and the basic reproduction number R0 = βijij where βij is the transmission coefficient of strain ij. Direct resource competition or strain-specific immunity may be relaxed by modifying the appropriate terms within the equation by a parameter ψ (0 ≤ ψ ≤ 1) specifying the degree of resistance against co-infection by the same metabolic type and a parameter γ (0 ≤ γ ≤ 1) specifying the level of strain-specific immunity:

When γ = 1, an individual who has previously been infected with strain a1 cannot subsequently be infected with any strain of antigenic type a. When γ = 0, an individual previously infected with strain a1 remains fully susceptible to all strains of antigenic type a. Thus, when γ = 1, we recover eqs (1) and (2) with fully protective type specific immunity while, at γ = 0, the only form of competition in the system for direct resources (ie. through sharing of metabolic type).

This framework may be extended along the same principles to accommodate additional serotypes and metabolic types as well as a third virulence locus, as described in S1 Text.

Vaccination may be included by adding the term v(1- zj) to the rate of change in proportiion immune to j if j is a serotype [43]. Other methods, such as a stepwise increase in zj may also be employed to the same effect.

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Štítky
Hygiena a epidemiológia Infekčné lekárstvo Laboratórium

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PLOS Pathogens


2015 Číslo 7
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Autori: MUDr. Tomáš Ürge, PhD.

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