#PAGE_PARAMS# #ADS_HEAD_SCRIPTS# #MICRODATA#

dMyc Functions Downstream of Yorkie to Promote the Supercompetitive Behavior of Hippo Pathway Mutant Cells


Genetic analyses in Drosophila epithelia have suggested that the phenomenon of “cell competition” could participate in organ homeostasis. It has been speculated that competition between different cell populations within a growing organ might play a role as either tumor promoter or tumor suppressor, depending on the cellular context. The evolutionarily conserved Hippo (Hpo) signaling pathway regulates organ size and prevents hyperplastic disease from flies to humans by restricting the activity of the transcriptional cofactor Yorkie (yki). Recent data indicate also that mutations in several Hpo pathway members provide cells with a competitive advantage by unknown mechanisms. Here we provide insight into the mechanism by which the Hpo pathway is linked to cell competition, by identifying dMyc as a target gene of the Hpo pathway, transcriptionally upregulated by the activity of Yki with different binding partners. We show that the cell-autonomous upregulation of dMyc is required for the supercompetitive behavior of Yki-expressing cells and Hpo pathway mutant cells, whereas the relative levels of dMyc between Hpo pathway mutant cells and wild-type neighboring cells are critical for determining whether cell competition promotes a tumor-suppressing or tumor-inducing behavior. All together, these data provide a paradigmatic example of cooperation between tumor suppressor genes and oncogenes in tumorigenesis and suggest a dual role for cell competition during tumor progression depending on the output of the genetic interactions occurring between confronted cells.


Vyšlo v časopise: dMyc Functions Downstream of Yorkie to Promote the Supercompetitive Behavior of Hippo Pathway Mutant Cells. PLoS Genet 6(9): e32767. doi:10.1371/journal.pgen.1001140
Kategorie: Research Article
prolekare.web.journal.doi_sk: https://doi.org/10.1371/journal.pgen.1001140

Souhrn

Genetic analyses in Drosophila epithelia have suggested that the phenomenon of “cell competition” could participate in organ homeostasis. It has been speculated that competition between different cell populations within a growing organ might play a role as either tumor promoter or tumor suppressor, depending on the cellular context. The evolutionarily conserved Hippo (Hpo) signaling pathway regulates organ size and prevents hyperplastic disease from flies to humans by restricting the activity of the transcriptional cofactor Yorkie (yki). Recent data indicate also that mutations in several Hpo pathway members provide cells with a competitive advantage by unknown mechanisms. Here we provide insight into the mechanism by which the Hpo pathway is linked to cell competition, by identifying dMyc as a target gene of the Hpo pathway, transcriptionally upregulated by the activity of Yki with different binding partners. We show that the cell-autonomous upregulation of dMyc is required for the supercompetitive behavior of Yki-expressing cells and Hpo pathway mutant cells, whereas the relative levels of dMyc between Hpo pathway mutant cells and wild-type neighboring cells are critical for determining whether cell competition promotes a tumor-suppressing or tumor-inducing behavior. All together, these data provide a paradigmatic example of cooperation between tumor suppressor genes and oncogenes in tumorigenesis and suggest a dual role for cell competition during tumor progression depending on the output of the genetic interactions occurring between confronted cells.


Zdroje

1. MorataG

RipollP

1975 Minutes: mutants of Drosophila autonomously affecting cell division rate. Dev Biol 42 211 221

2. MorenoE

2008 Is cell competition relevant to cancer? Nat Rev Cancer 8 141 147

3. MartínFA

HerreraFC

MorataG

2009 Cell competition, growth and size control in the Drosophila wing imaginal disc. Development 136 3747 3756

4. de la CovaC

AbrilM

BellostaP

GallantP

JohnstonLA

2004 Drosophila myc regulates organ size by inducing cell competition. Cell 117 107 116

5. MorenoE

BaslerK

2004 dMyc transforms cells into super-competitors. Cell 117 117 129

6. BakerNE

2008 Cell competition and its possible relation to cancer. Cancer Res 68 50505 50507

7. FroldiF

ZiosiM

GaroiaF

PessionA

GrzeschikNA

2010 The lethal giant larvae tumour suppressor mutation requires dMyc oncoprotein to promote clonal malignancy. BMC Biol 8 33

8. OsterSK

HoCS

SoucieEL

PennLZ

2002 The myc oncogene: Marvelous Complex. Adv Cancer Res 84 81 154

9. JohnstonLA

ProberDA

EdgarBA

EisenmanRN

GallantP

1999 Drosophila myc regulates cellular growth during development. Cell 98 779 790

10. MeyerN

KimSS

PennLZ

2006 The Oscar-worthy role of Myc in apoptosis. Semin Cancer Biol 16 275 287

11. MonteroL

MüllerN

GallantP

2008 Induction of Apoptosis by Drosophila Myc. Genesis 46 104 111

12. GrewalSS

LiL

OrianA

EisenmanRN

EdgarBA

2005 Myc-dependent regulation of ribosomal RNA synthesis during Drosophila development. Nat Cell Biol 7 295 302

13. VitaM

HenrikssonM

2006 The Myc oncoprotein as a therapeutic target for human cancer. Semin Cancer Biol 16 318 330

14. LandH

ParadaLF

WeinbergRA

1983 Cellular oncogenes and multistep carcinogenesis. Science 222 771 778

15. ZhanL

RosenbergA

BergamiKC

YuM

XuanZ

2008 Deregulation of Scribble Promotes Mammary Tumorigenesis and Reveals a Role for Cell Polarity in Carcinoma. Cell 135 865 878

16. HuangJ

WuS

BarreraJ

MatthewsK

PanD

2005 The Hippo signaling pathway coordinately regulates cell proliferation and apoptosis by inactivating Yorkie, the Drosophila homolog of YAP. Cell 122 421 434

17. DongJ

FeldmannG

HuangJ

WuS

ZhangN

2007 Elucidation of a universal size-control mechanism in Drosophila and mammals. Cell 130 1120 1133

18. SaucedoLJ

EdgarB

2007 Filling out the Hippo pathway. Nat Rev Mol Cell Biol 8 613 621

19. TylerDM

LiW

ZhuoN

PellockB

BakerNE

2006 Genes affecting cell competition in Drosophila. Genetics 175 643 657

20. HarveyK

TaponN

2007 The Salvador-Warts-Hippo pathway - an emerging tumour-suppressor network. Nat Rev Cancer 3 182 191

21. OhH

IrvineKD

2009 In vivo analysis of Yorkie phosphorilation sites. Oncogene 28 1916 1927

22. RenF

ZhangL

JiangJ

2009 Hippo signaling regulates Yorkie nuclear localization and activity through 14-3-3 dependent and independent mechanisms. Dev Biol 337 303 312

23. NicolayBN

FrolovMV

2008 Context-dependent requirement for dE2F during oncogenic proliferation. PLoS Genet 4 e1000205

24. NoloR

MorrisonCM

TaoC

ZhangX

HalderG

2006 The bantam MicroRNA is a target of the Hippo tumor-suppressor pathway. Curr Biol 16 1895 1904

25. ThompsonBJ

CohenSM

2006 The Hippo pathway regulates the bantam microRNA to control cell proliferation and apoptosis in Drosophila. Cell 126 767 774

26. Baena-LopezLA

RodriguezI

BaonzaA

2008 The tumor suppressor genes dachsous and fat modulate different signalling pathways by regulating dally and dally-like. Proc Natl Acad Sci USA 105 9645 9650

27. GoulevY

FaunyJD

Gonzalez-MartiB

FlagielloD

SilberJ

2008 SCALLOPED Interacts with YORKIE, the Nuclear Effector of the Hippo Tumor-Suppressor Pathway in Drosophila. Curr Biol 18 435 441

28. WuS

LiuY

ZhengQ

DongJ

PanD

2008 The TEAD/TEF family protein Scalopped mediates transcriptional output of the Hippo growth-regulatory pathway. Dev Cell 14 388 398

29. ZhangL

RenF

ZhangQ

ChenY

WangB

2008 The TEAD/TEF family of transcription factor Scalopped mediates Hippo signaling in organ size control. Dev Cell 14 377 387

30. ZhaoB

YeX

YuJ

LiL

LiW

2008 TEAD mediates YAP-dependent gene induction and growth control. Genes Dev 22 1962 1971

31. PengHW

SlatteryM

MannRS

2009 Transcription factor choice in the Hippo signaling pathway: homothorax and yorkie regulation of the microRNA bantam, in the progenitor domain of the Drosophila eye imaginal disc. Genes Dev 1 2307 2319

32. WilleckeM

HamaratogluF

Kango-SinghM

UdanR

ChenC

2006 The Fat Cadherin Acts through the Hippo Tumor-Suppressor Pathway to Regulate Tissue Size. Curr Biol 16 1 11

33. SilvaE

TsatskisY

GardanoL

TaponN

McNeillH

2006 The Tumor-Suppressor Gene fat Controls Tissue Growth Upstream of Expanded in the Hippo Signaling Pathway. Curr Biol 16 2081 2089

34. ChoE

FengY

RauskolbC

MaitraS

FehonR

2006 Delineation of a Fat tumor suppressor pathway. Nat Genet 38 1142 1150

35. BennettFC

HarveyKF

2006 Fat Cadherin Modulates Organ Size in Drosophila via the Salvador/Warts/Hippo Signaling Pathway. Curr Biol 16 2101 2110

36. ChoE

IrvineKD

2004 Action of fat, four-jointed, dachsous and dachs in distal-to-proximal wing signaling. Development 131 4489 4500

37. FengY

IrvineKD

2007 Fat and expanded act in parallel to regulate growth through warts. Proc Natl Acad Sci USA 104 20362 20367

38. WilleckeM

HamaratogluF

Sansores-GarciaL

TaoC

HalderG

2008 Boundaries of Dachsous Cadherin activity modulate the Hippo signaling pathway to induce cell proliferation. Proc Natl Acad Sci USA 105 14897 14902

39. HamaratogluF

WilleckeM

Kango-SinghM

NoloR

HyunE

2006 The tumour suppressor genes NF2/Merlin and Expanded act through Hippo signalling to regulate cell proliferation and apoptosis. Nat Cell Biol 8 27 36

40. WuS

HuangJ

DongJ

PanD

2003 hippo encodes a Ste-20 family protein kinase that restricts cell proliferation and promotes apoptosis in conjunction with salvador and warts. Cell 114 445 456

41. ReddyBVVG

IrvineKD

2008 The Fat and warts signaling pathways: new insights into their regulation, mechanism and conservation. Development 135 2827 2838

42. MorenoE

BaslerK

MorataG

2002 Cells compete for decapentaplegic survival factor to prevent apoptosis in Drosophila wing development. Nature 416 755 759

43. LiW

BakerNE

2007 Engulfment is required for cell competition. Cell 15 1215 1225

44. Senoo-MatsudaN

JohnstonLA

2007 Soluble factors mediate competitive and cooperative interactions between cells expressing different levels of Drosophila Myc. Proc Natl Acad Sci USA 104 18543 18548

45. GaroiaF

GrifoniD

TrottaV

GuerraD

PezzoliMC

2005 The tumor suppressor gene fat modulates the EGFR-mediated proliferation control in the imaginal tissues of Drosophila melanogaster. Mech Dev 122 175 187

46. PeterA

SchöttlerP

WernerM

BeinertN

DoweG

2002 Mapping and identification of essential gene functions on the X chromosome of Drosophila. EMBO Rep 31 34 38

47. CrannaN

QuinnL

2009 Impact of steroid hormone signals on Drosophila cell cycle during development. Cell Div 20 4:3

48. XiaoJH

DavidsonI

MatthesH

GarnierJM

ChambonP

1991 Cloning, expression, and transcriptional properties of the human enhancer factor TEF-1. Cell 65 551 568

49. LarkinSB

FarranceIK

OrdahlCP

1996 Flanking sequences modulate the cell specificity of M-CAT elements. Mol Cell Biol 16 3742 3755

50. BourbonHM

Gonzy-TreboulG

PeronnetF

AlinMF

ArdourelC

2002 A P-insertion screen identifying novel X-linked essential genes in Drosophila. Mech Dev 110 71 83

51. BenassayagC

MonteroL

ColombieN

GallantP

CribbsD

2005 Human c-Myc isoforms differentially regulate cell growth and apoptosis in Drosophila melanogaster. Mol Cell Biol 25 9897 9909

52. MartínFA

Peréz-GarijoA

MorataG

2009 Apoptosis in Drosophila: compensatory proliferation and undead cells. Int J Dev Biol 53 1341 1347

53. OhH

IrvineKD

2008 In vivo regulation of Yorkie phosphorylation and localization. Development 135 1081 1088

54. Lam-HimlinDM

DanielsJA

GayyedMF

DongJ

MaitraA

2006 The hippo pathway in human upper gastrointestinal dysplasia and carcinoma: a novel oncogenic pathway. Int J Gastrointest Cancer 37 103 109

55. SteinhardtAA

GayyedMF

KleinAP

DongJ

MaitraA

2008 Expression of Yes-associated protein in common solid tumors. Hum Pathol 39 1582 1589

56. OverholtzerM

ZhangJ

SmolenGA

MuirB

LiW

2006 Transforming properties of YAP, a candidate oncogene on the chromosome 11q22 amplicon. Proc Natl Acad Sci USA 103 12405 12410

57. KurantE

PaiCY

SharfR

HalachmiN

SunYH

1998 Dorsotonals/homothorax, the Drosophila homologue of meis1, interacts with extradenticle in patterning of the embryonic PNS. Development 125 1037 1048

58. JohnstonLA

EdgarBA

1998 Wingless and Notch regulate cell-cycle arrest in the developing Drosophila wing. Nature 394 82 84

59. De la CovaC

JohnstonLA

2006 Myc in model organisms: a view from the fly room. Sem Cancer Biol 16 303 312

Štítky
Genetika Reprodukčná medicína

Článok vyšiel v časopise

PLOS Genetics


2010 Číslo 9
Najčítanejšie tento týždeň
Najčítanejšie v tomto čísle
Kurzy

Zvýšte si kvalifikáciu online z pohodlia domova

Aktuální možnosti diagnostiky a léčby litiáz
nový kurz
Autori: MUDr. Tomáš Ürge, PhD.

Všetky kurzy
Prihlásenie
Zabudnuté heslo

Zadajte e-mailovú adresu, s ktorou ste vytvárali účet. Budú Vám na ňu zasielané informácie k nastaveniu nového hesla.

Prihlásenie

Nemáte účet?  Registrujte sa

#ADS_BOTTOM_SCRIPTS#