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Crimean-Congo Hemorrhagic Fever Virus Entry into Host Cells Occurs through the Multivesicular Body and Requires ESCRT Regulators


Crimean-Congo hemorrhagic fever virus (CCHFV) is the cause of a severe, often fatal disease in humans. While it has been demonstrated that CCHFV cell entry depends on clathrin-mediated endocytosis, low pH, and early endosomes, the identity of the endosomes where virus penetrates into cell cytoplasm to initiate genome replication is unknown. Here, we showed that CCHFV was transported through early endosomes to multivesicular bodies (MVBs). We also showed that MVBs were likely the last organelle virus encountered before escaping into the cytoplasm. Our work has identified new cellular factors essential for CCHFV entry and potential novel targets for therapeutic intervention against this pathogen.


Vyšlo v časopise: Crimean-Congo Hemorrhagic Fever Virus Entry into Host Cells Occurs through the Multivesicular Body and Requires ESCRT Regulators. PLoS Pathog 10(9): e32767. doi:10.1371/journal.ppat.1004390
Kategorie: Research Article
prolekare.web.journal.doi_sk: https://doi.org/10.1371/journal.ppat.1004390

Souhrn

Crimean-Congo hemorrhagic fever virus (CCHFV) is the cause of a severe, often fatal disease in humans. While it has been demonstrated that CCHFV cell entry depends on clathrin-mediated endocytosis, low pH, and early endosomes, the identity of the endosomes where virus penetrates into cell cytoplasm to initiate genome replication is unknown. Here, we showed that CCHFV was transported through early endosomes to multivesicular bodies (MVBs). We also showed that MVBs were likely the last organelle virus encountered before escaping into the cytoplasm. Our work has identified new cellular factors essential for CCHFV entry and potential novel targets for therapeutic intervention against this pathogen.


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Hygiena a epidemiológia Infekčné lekárstvo Laboratórium

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PLOS Pathogens


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