Structural Correlates of Rotavirus Cell Entry

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Non-enveloped viruses (viruses lacking a lipid-bilayer membrane) require local disruption of a cellular membrane to gain access to the cell interior and thereby initiate infection. Most double-strand RNA viruses have an outer protein layer that mediates this entry step and an inner-capsid particle that transcribes their segmented dsRNA genomes and extrudes the capped mRNAs into the cytosol. Removing the two rotavirus outer-layer proteins inactivates the virus, but recoating with recombinant outer-layer proteins restores infectivity. We have labeled the recombinant proteins with distinct fluorophores and the stripped inner-capsid particle with a third fluorophore and reconstituted fully infectious particles from the labeled components. We have followed by live-cell imaging the binding and engulfment of the labeled particles and studied the kinetics of inner-capsid particle release. We have interpreted these events in structural terms by examining images of entering particles from conventional electron microscopy and electron cryotomography. When analyzed in view of our previously determined high resolution structures of the virus particle and its constituents, and of information about conformational changes in the outer-layer components, our data lead to a molecular description of the observed entry steps and of the mechanism of membrane disruption.

Vyšlo v časopise: Structural Correlates of Rotavirus Cell Entry. PLoS Pathog 10(9): e32767. doi:10.1371/journal.ppat.1004355
Kategorie: Research Article
prolekare.web.journal.doi_sk: https://doi.org/10.1371/journal.ppat.1004355

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