Reorganization of the Endosomal System in -Infected Cells: The Ultrastructure of -Induced Tubular Compartments

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Salmonella enterica is an invasive, facultative intracellular bacterial pathogen. Within mammalian host cells, Salmonella inhabits a specialized membrane-bound compartment, the Salmonella-containing vacuole (SCV), redirects host cell vesicular transport and massively remodels the endosomal system. These activities depend on the function of a type III secretion system and its translocated effector proteins. Intracellular Salmonella induces several types of tubular compartments termed Salmonella-induced tubules (SIT), but the biogenesis and biological function of SIT is only partially understood. Our work combines live cell imaging with correlative light and electron microscopy to provide ultrastructural insight into SIT. We report that SIT emerge as single membrane tubules that convert into double membrane tubules entrapping cytosol and cytoskeletal filaments. Labeling of the endosomal compartment and cytochemistry demonstrate that the space between inner and outer SIT membrane is composed of internalized material and connected to Salmonella within the SCV. The effector proteins SseF and SseG translocated by intracellular Salmonella are essential for the conversion of single to double membrane SIT. These findings challenge current models for the intracellular lifestyle of Salmonella and the composition of its intracellular habitat.

Vyšlo v časopise: Reorganization of the Endosomal System in -Infected Cells: The Ultrastructure of -Induced Tubular Compartments. PLoS Pathog 10(9): e32767. doi:10.1371/journal.ppat.1004374
Kategorie: Research Article
prolekare.web.journal.doi_sk: https://doi.org/10.1371/journal.ppat.1004374

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