Small GTPase Rab21 Mediates Fibronectin Induced Actin Reorganization in : Implications in Pathogen Invasion
Entamoeba histolytica is one of the major causes of morbidity and mortality in developing economies. Severe amoebic infection leads to metastatic spread of the pathogen to extra intestinal sites, especially the liver, causing hepatic abscess. The migratory ability of the pathogen contributes to the spread of the disease. Here, we report that Rab21, a Ras superfamily GTPase, promotes actin dot formation under the fibronectin induced signal in E.histolytica. The amoebic actin dots share structural components and functional properties to a class of actin rich degradative counterparts found in higher eukaryotes called “invadosomes”. Invadosomes are associated with both normal animal development and diseased state such as cancer. We also show that collagen type I, another major ECM component, suppresses the genesis of actin dots, possibly through interaction with amoebic cell surface adhesion molecule, the Gal/GalNAc lectin complex. Based on our observations, we propose that Rab21 may play an important role in in vivo tissue invasion by the parasite which may have further implication in its virulence.
Vyšlo v časopise:
Small GTPase Rab21 Mediates Fibronectin Induced Actin Reorganization in : Implications in Pathogen Invasion. PLoS Pathog 11(3): e32767. doi:10.1371/journal.ppat.1004666
Kategorie:
Research Article
prolekare.web.journal.doi_sk:
https://doi.org/10.1371/journal.ppat.1004666
Souhrn
Entamoeba histolytica is one of the major causes of morbidity and mortality in developing economies. Severe amoebic infection leads to metastatic spread of the pathogen to extra intestinal sites, especially the liver, causing hepatic abscess. The migratory ability of the pathogen contributes to the spread of the disease. Here, we report that Rab21, a Ras superfamily GTPase, promotes actin dot formation under the fibronectin induced signal in E.histolytica. The amoebic actin dots share structural components and functional properties to a class of actin rich degradative counterparts found in higher eukaryotes called “invadosomes”. Invadosomes are associated with both normal animal development and diseased state such as cancer. We also show that collagen type I, another major ECM component, suppresses the genesis of actin dots, possibly through interaction with amoebic cell surface adhesion molecule, the Gal/GalNAc lectin complex. Based on our observations, we propose that Rab21 may play an important role in in vivo tissue invasion by the parasite which may have further implication in its virulence.
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Hygiena a epidemiológia Infekčné lekárstvo LaboratóriumČlánok vyšiel v časopise
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