RECG Maintains Plastid and Mitochondrial Genome Stability by Suppressing Extensive Recombination between Short Dispersed Repeats

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Recombinational DNA repair plays an important role in the maintenance of genomic stability by repairing DNA double-strand breaks and stalled replication forks. However, recombination between nonallelic similar sequences such as dispersed repeated sequences results in genomic instability. Plant plastid and mitochondrial genomes are compact (generally approximately 100–500 kb in size), but they contain essential genes. A substantial number of repeats are dispersed in these genomes, particularly in the mitochondrial genome. In this study, we showed that a knockout mutation of the newly identified plant-specific homolog of bacterial RecG DNA helicase RECG caused some defects in plastids and significant defects in the mitochondria. The organelle genomes in these mutants were destabilized by induced aberrant recombination between short (<100 bp) dispersed repeats. Recombination was induced at repeats as short as 8 bp. This suggests that RECG maintains plastid and mitochondrial genome stability by suppressing aberrant recombination between short dispersed repeats. Because such a phenomenon, to our knowledge, has not been observed in bacterial recG mutants, our results suggest an organelle-specific genome maintenance system distinct from that of bacteria.

Vyšlo v časopise: RECG Maintains Plastid and Mitochondrial Genome Stability by Suppressing Extensive Recombination between Short Dispersed Repeats. PLoS Genet 11(3): e32767. doi:10.1371/journal.pgen.1005080
Kategorie: Research Article
prolekare.web.journal.doi_sk: https://doi.org/10.1371/journal.pgen.1005080

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