Construction of a high-density genetic map and fine mapping of a candidate gene locus for a novel branched-spike mutant in barley
Autoři:
Weibin Wang aff001; Junyu He aff001; Shengwei Chen aff001; Peng Peng aff001; Wei Zhong aff001; Xintian Wang aff001; Tingting Zhang aff001; Yuping Li aff001
Působiště autorů:
College of Agronomy and Biotechnology, Yunnan Agricultural University, Kunming, Yunnan, China
aff001
Vyšlo v časopise:
PLoS ONE 15(1)
Kategorie:
Research Article
prolekare.web.journal.doi_sk:
https://doi.org/10.1371/journal.pone.0227617
Souhrn
A Yunnan branched-spike (Ynbs) barley mutant is useful for study of the genetic mechanisms underlying variation in barley spike architecture. In the current study, a mutant (Ynbs-1), a recombinant inbred line (RIL-1), and a cultivar (BDM-8) were used as parents to develop populations. Ynbs-1 exhibits typical branched spike, whereas the others exhibit six-row spike. Genetic analysis on their F1, F2 and F3 populations showed that one recessive gene is responsible for the branched spike trait. SLAF marker generated from specific locus amplified fragment sequencing (SLAF-seq) was used to genotype the populations. A high-density genetic map of barley was constructed using 14,348 SLAF markers, which covered all 7 chromosomes at 1,347.44 cM in length with an average marker density of 0.09 cM between adjacent markers. Linkage analysis of the branched-spike trait using the genetic map indicated that branched spike trait in the Ynbs-1 is controlled by single locus on chromosome 2H at the interval between 65.00 and 65.47 cM that is flanked by Marker310119 and Marker2679451. Several candidate genes that may be responsible for barley multiple-spikelet degeneration, single-floret spikelet increase and seed set rate decrease were identified in the region. The high-density genetic map and the gene locus revealed in this study provide valuable information for elucidating the genetic mechanism of spike branching in barley.
Klíčová slova:
Rice – Genetic loci – Quantitative trait loci – Gene mapping – Genetic linkage – Linkage mapping – Chromosome mapping – Barley
Zdroje
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