Fast quantitative time lapse displacement imaging of endothelial cell invasion
Autoři:
Christian Steuwe aff001; Marie-Mo Vaeyens aff002; Alvaro Jorge-Peñas aff002; Célie Cokelaere aff001; Johan Hofkens aff003; Maarten B. J. Roeffaers aff001; Hans Van Oosterwyck aff002
Působiště autorů:
Centre for Membrane Separations, Adsorption, Catalysis and Spectroscopy for Sustainable Solutions (cMACS), Department of Microbial and Molecular Systems (MS), KU Leuven, Leuven, Belgium
aff001; Biomechanics Section (BMe), Department of Mechanical Engineering, KU Leuven, Leuven, Belgium
aff002; Molecular Imaging and Photonics, Department of Chemistry, KU Leuven, Leuven, Belgium
aff003; Prometheus, Division of Skeletal Tissue Engineering, KU Leuven, Leuven, Belgium
aff004
Vyšlo v časopise:
PLoS ONE 15(1)
Kategorie:
Research Article
prolekare.web.journal.doi_sk:
https://doi.org/10.1371/journal.pone.0227286
Souhrn
In order to unravel rapid mechano-chemical feedback mechanisms in sprouting angiogenesis, we combine selective plane illumination microscopy (SPIM) and tailored image registration algorithms — further referred to as SPIM-based displacement microscopy — with an in vitro model of angiogenesis. SPIM successfully tackles the problem of imaging large volumes while upholding the spatial resolution required for the analysis of matrix displacements at a subcellular level. Applied to in vitro angiogenic sprouts, this unique methodological combination relates subcellular activity — minute to second time scale growing and retracting of protrusions — of a multicellular systems to the surrounding matrix deformations with an exceptional temporal resolution of 1 minute for a stack with multiple sprouts simultaneously or every 4 seconds for a single sprout, which is 20 times faster than with a conventional confocal setup. Our study reveals collective but non-synchronised, non-continuous activity of adjacent sprouting cells along with correlations between matrix deformations and protrusion dynamics.
Klíčová slova:
Light – Collagens – Fluorescence microscopy – Fluorescence imaging – Deformation – Optical lenses – Gels – Angiogenesis
Zdroje
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