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preLekára.sk / Odborné časopisy / PLOS One / 2020 - 1

Severe thiamine deficiency in eastern Baltic cod (Gadus morhua)

English version

Autoři: Josefin Engelhardt aff001;  Oscar Frisell aff001;  Hanna Gustavsson aff001;  Tomas Hansson aff001;  Rajlie Sjöberg aff002;  Tracy K. Collier aff003;  Lennart Balk aff001
Působiště autorů: Department of Environmental Science and Analytical Chemistry, Stockholm University, Stockholm, Sweden aff001;  Institute of Marine Research, Swedish University of Agricultural Sciences, Lysekil, Sweden aff002;  Huxley College of the Environment, Western Washington University, Bellingham, Washington, United States of America aff003
Vyšlo v časopise: PLoS ONE 15(1)
Kategorie: Research Article
prolekare.web.journal.doi_sk: https://doi.org/10.1371/journal.pone.0227201

Souhrn

The eastern Baltic cod (Gadus morhua) population has been decreasing in the Baltic Sea for at least 30 years. Condition indices of the Baltic cod have decreased, and previous studies have suggested that this might be due to overfishing, predation, lower dissolved oxygen or changes in salinity. However, numerous studies from the Baltic Sea have demonstrated an ongoing thiamine deficiency in several animal classes, both invertebrates and vertebrates. The thiamine status of the eastern Baltic cod was investigated to determine if thiamine deficiency might be a factor in ongoing population declines. Thiamine concentrations were determined by chemical analyses of thiamine, thiamine monophosphate and thiamine diphosphate (combined SumT) in the liver using high performance liquid chromatography. Biochemical analyses measured the activity of the thiamine diphosphate-dependent enzyme transketolase to determine the proportion of apoenzymes in both liver and brain tissue. These biochemical analyses showed that 77% of the cod were thiamine deficient in the liver, of which 13% had a severe thiamine deficiency (i.e. 25% transketolase enzymes lacked thiamine diphosphate). The brain tissue of 77% of the cod showed thiamine deficiency, of which 64% showed severe thiamine deficiency. The thiamine deficiency biomarkers were investigated to find correlations to different biological parameters, such as length, weight, otolith weight, age (annuli counting) and different organ weights. The results suggested that thiamine deficiency increased with age. The SumT concentration ranged between 2.4–24 nmol/g in the liver, where the specimens with heavier otoliths had lower values of SumT (P = 0.0031). Of the cod sampled, only 2% of the specimens had a Fulton’s condition factor indicating a healthy specimen, and 49% had a condition factor below 0.8, indicating poor health status. These results, showing a severe thiamine deficiency in eastern Baltic cod from the only known area where spawning presently occurs for this species, are of grave concern.

Klíčová slova:

Gonads – Biochemical analysis – Spawning – Thiamine – Cod – Eels – Otolith – Baltic Sea

Zdroje

1. Goyer A. Thiamine in plants: aspects of its metabolism and functions. Phytochemistry. 2010 Oct;71(14–15):1615–24. doi: 10.1016/j.phytochem.2010.06.022 20655074

2. Bellyei S, Szigeti A, Boronkai A, Szabo Z, Bene J, Janaky T, et al. Cloning, sequencing, structural and molecular biological characterization of placental protein 20 (PP20)/human thiamin pyrophosphokinase (hTPK). Placenta. 2005 Jan;26(1):34–46. doi: 10.1016/j.placenta.2004.03.008 15664409

3. Harper AE, Miller R, Block KP. Branched-chain amino acid metabolism. Annu Rev Nutr. 1984 Jul;4(1):409–54.

4. Navarro D, Zwingmann C, Butterworth RF. Impaired oxidation of branched-chain amino acids in the medial thalamus of thiamine-deficient rats. Metab Brain Dis. 2008 Dec;23(4):445–55. doi: 10.1007/s11011-008-9105-6 18773288

5. Foulon V, Antonenkov VD, Croes K, Waelkens E, Mannaerts GP, Van Veldhoven PP, et al. Purification, molecular cloning, and expression of 2-hydroxyphytanoyl-CoA lyase, a peroxisomal thiamine pyrophosphate-dependent enzyme that catalyzes the carbon-carbon bond cleavage during α-oxidation of 3-methyl-branched fatty acids. PNAS. 1999 Aug;96(18):10039–44. doi: 10.1073/pnas.96.18.10039 10468558

6. Sniekers M, Foulon V, Mannaerts GP, Van Maldergem L, Mandel H, Gelb BD, et al. Thiamine pyrophosphate: An essential cofactor for the α-oxidation in mammals—implications for thiamine deficiencies? Cell Mol Life Sci. 2006 Jul;63(13):1553–63. doi: 10.1007/s00018-005-5603-4 16786225

7. Lehninger AL, Nelson DL, Cox MM. Principles of biochemistry. 5th ed. San Francisco: W.H. Freeman; 2005.

8. Nilsson U, Meshalkina L, Lindqvist Y, Schneider G. Examination of substrate binding in thiamin diphosphate-dependent transketolase by protein crystallography and site-directed mutagenesis. J Biol Chem. 1997 Jan;272(3):1864–9. doi: 10.1074/jbc.272.3.1864 8999873

9. Berg JM, Tymoczko JL, Stryer L. Biochemistry. 7th ed. New York: W.H. Freeman; 2012.

10. Wieland OH. The mammalian pyruvate dehydrogenase complex: structure and regulation. Rev Physiol Biochem Pharmacol. 1983; 96:123–70. doi: 10.1007/bfb0031008 6338572

11. Arjunan P, Nemeria N, Brunskill A, Chandrasekhar K, Sax M, Yan Y, et al. Structure of the pyruvate dehydrogenase multienzyme complex E1 component from Escherichia coli at 1.85 Å resolution. Biochemistry. 2002 Apr;41(16):5213–21. doi: 10.1021/bi0118557 11955070

12. Hamada S, Hirashima H, Imaeda M, Okamoto Y, Hamaguchi-Hamada K, Kurumata-Shigeto M. Thiamine deficiency induces massive cell death in the olfactory bulbs of mice. J Neuropathol Exp Neurol. 2013 Dec;72(12):1193–202. doi: 10.1097/NEN.0000000000000017 24226264

13. Butterworth RF, Giguère JF, Besnard AM. Activities of thiamine-dependent enzymes in two experimental models of thiamine-deficiency encephalopathy 2. α-ketoglutarate dehydrogenase. Neurochem Res. 1986 Apr;11(4):567–77. doi: 10.1007/bf00965326 3724963

14. Lai JC, Cooper AJ. Brain α-ketoglutarate dehydrogenase complex: kinetic properties, regional distribution, and effects of inhibitors. J Neurochem. 1986 Nov;47(5):1376–86. doi: 10.1111/j.1471-4159.1986.tb00768.x 3760866

15. Åkerman G, Balk L. Descriptive studies of mortality and morphological disorders in early life stages of cod and salmon originating from the Baltic Sea. Am Fish Soc Symp. 1998;21:41–61.

16. Balk L, Hägerroth P-Å, Åkerman G, Hanson M, Tjärnlund U, Hansson T, et al. Wild birds of declining European species are dying from a thiamine deficiency syndrome. Proc Natl Acad Sci. 2009 Jul;106(29):12001–6. doi: 10.1073/pnas.0902903106 19597145

17. Balaghi M, Pearson WN. Tissue and intracellular distribution of radioactive thiamine in normal and thiamine-deficient rats. J Nutr. 1966 Jun;89(2):127–32. doi: 10.1093/jn/89.2.127 5947519

18. Shangari N, Bruce WR, Poon R, O’brien PJ. Toxicity of glyoxals—role of oxidative stress, metabolic detoxification and thiamine deficiency. Biochem Soc Trans. 2003 Dec;31(6):1390–3.

19. Campbell C. The severe lactic acidosis of thiamine deficiency: acute pernicious or fulminating beriberi. The Lancet. 1984 Aug;324(8400):446–9.

20. Schönfeld P, Struy H. Refsum disease diagnostic marker phytanic acid alters the physical state of membrane proteins of liver mitochondria. FEBS letters. 1999 Aug;457(2):179–83. doi: 10.1016/s0014-5793(99)01009-1 10471774

21. Wanders RJ, Jansen GA, Skjeldal OH. Refsum disease, peroxisomes and phytanic acid oxidation: a review. J Neuropathol Exp Neurol. 2001 Nov;60(11):1021–31. doi: 10.1093/jnen/60.11.1021 11706932

22. Busanello EN, Amaral AU, Tonin AM, ZanattaÂ, Viegas CM, Vargas CR, et al. Disruption of mitochondrial homeostasis by phytanic acid in cerebellum of young rats. The Cerebellum. 2013 Jun;12(3):362–9. doi: 10.1007/s12311-012-0426-y 23081695

23. Singleton CK, Martin PR. Molecular mechanisms of thiamine utilization. Curr Mol Med. 2001 May;1(2):197–207. doi: 10.2174/1566524013363870 11899071

24. Combs GF. The Vitamins. 3rd Edition. Oxford, UK: Elsevier Inc.;2008

25. Manzetti S, Zhang J, van der Spoel D. Thiamin function, metabolism, uptake, and transport. Biochemistry. 2014 Jan;53(5):821–35. doi: 10.1021/bi401618y 24460461

26. Lee BJ, Jaroszewska M, Dabrowski K, Czesny S, Rinchard J. Effects of vitamin B1 (thiamine) deficiency in lake trout alevins and preventive treatments. J Aquat Anim Health. 2009 Dec;21(4):290–301. doi: 10.1577/H07-053.1 20218503

27. Fitzsimons JD, Brown SB, Williston B, Williston G, Brown LR, Moore K, et al. Influence of thiamine deficiency on lake trout larval growth, foraging, and predator avoidance. J Aquat Anim Health. 2009 Dec;21(4):302–14. doi: 10.1577/H08-019.1 20218504

28. Carvalho PS, Tillitt DE, Zajicek JL, Claunch RA, Honeyfield DC, Fitzsimons JD, et al. Thiamine deficiency effects on the vision and foraging ability of lake trout fry. J Aquat Anim Health. 2009 Dec;21(4):315–25. doi: 10.1577/H08-025.1 20218505

29. Fitzsimons JD. The effect of B-vitamins on a swim-up syndrome in Lake Ontario lake trout. J Great Lakes Res. 1995;21(1):286–9.

30. Paton DC, Dorward DF, Fell P. Thiamine deficiency and winter mortality in red wattlebirds, Anthochaera carunculata (aves: meliphagidae) in surburban Melbourne. Aust J Zool. 1983;31(2):147–54.

31. Fitzsimons JD, Brown SB, Brown LR, Verreault G, Tardif R, Drouillard KG, et al. Impacts of diet on thiamine status of Lake Ontario American eels. Trans Am Fish Soc. 2013 Sep;142(5):1358–69.

32. Balk L, Hägerroth PÅ, Gustavsson H, Sigg L, Åkerman G, Muñoz YR, et al. Widespread episodic thiamine deficiency in Northern Hemisphere wildlife. Sci Rep. 2016 Dec;6:38821. doi: 10.1038/srep38821 27958327

33. Amcoff P, Börjeson H, Landergren P, Vallin L, Norrgren L. Thiamine (vitamin B1) concentrations in salmon (Salmo salar), brown trout (Salmo trutta) and cod (Gadus morhua) from the Baltic Sea. Ambio. 1999 Feb;28(1):48–54.

34. Honeyfield DC, Murphy JM, Howard KG, Strasburger WW, Matz AC. An exploratory assessment of thiamine status in western Alaska Chinook salmon (Oncorhynchus tshawytscha). North Pacific Anadromous Fish Commission Bulletin No. 2016 Dec;6:21–31.

35. Brown SB, Honeyfield DC, Hnath JG, Wolgamood M, Marcquenski SV, Fitzsimons JD, et al. Thiamine status in adult salmonines in the Great Lakes. J Aquat Anim Health. 2005 Mar;17(1):59–64.

36. Brown SB, Fitzsimons JD, Palace VP, Vandenbyllaardt L. Thiamine and early mortality syndrome in lake trout. Am Fish Soc Symp. 1998;21:18–25.

37. Fitzsimons JD, Vandenbyllaardt L, Brown SB. The use of thiamine and thiamine antagonists to investigate the etiology of early mortality syndrome in lake trout (Salvelinus namaycush). Aquat Toxicol. 2001 May;52(3–4):229–39. doi: 10.1016/s0166-445x(00)00147-8 11239684

38. Sepúlveda MS, Wiebe JJ, Honeyfield DC, Rauschenberger HR, Hinterkopf JP, Johnson WE, et al. Organochlorine pesticides and thiamine in eggs of largemouth bass and American alligators and their relationship with early life-stage mortality. J Wildl Dis. 2004 Oct;40(4):782–6. doi: 10.7589/0090-3558-40.4.782 15650100

39. Sutherland WJ, Butchart SH, Connor B, Culshaw C, Dicks LV, Dinsdale J, et al. A 2018 horizon scan of emerging issues for global conservation and biological diversity. Trends Ecol Evol. 2018 Jan;33(1):47–58. doi: 10.1016/j.tree.2017.11.006 29217396

40. Bagge O, Thurow F. The Baltic cod stock: fluctuations and possible causes. ICES Marine Science Symposia. 1994;198:254–68.

41. Köster FW, Huwer B, Hinrichsen HH, Neumann V, Makarchouk A, Eero M, et al. Eastern Baltic cod recruitment revisited -dynamics and impacting factors. ICES Journal of Marine Science. 2017 Jan;74(1):3–19.

42. ICES Cod (Gadus morhua) in subdivisions 24–32, eastern Baltic stock (eastern Baltic Sea) ICES Advice on fishing opportunities, catch, and effort. 2018.cod;27.24–32, https://doi.org/10.17895/ices.pub.4378.

43. ICES Cod (Gadus morhua) in subdivisions 24–32, eastern Baltic stock (eastern Baltic Sea) Report of the ICES Advisory Committee, 2019; cod;27.24–32, https://doi.org/10.17895/ices.advice.4747.

44. Fångststatistik yrkesfisket (Catch statistics Commercial fishing) [Internet]. Gothenburg, Sweden: Swedish Agency for Marine and Water Management; 1999-. [cited 2019 Feb 27]. https://www.havochvatten.se/hav/samordning-fakta/data-statistik/fangststatistik-yrkesfisket.html

45. Svedäng H, Hornborg S. Historic changes in length distributions of three Baltic cod (Gadus morhua) stocks: Evidence of growth retardation. Ecology and evolution. 2017 Aug;7(16):6089–102. doi: 10.1002/ece3.3173 28861215

46. Eero M, Hjelm J, Behrens J, Buchmann K, Cardinale M, Casini M, et al. Eastern Baltic cod in distress: biological changes and challenges for stock assessment. ICES Journal of Marine Science. 2015 Jun;72(8):2180–6.

47. Vainikka A, Gårdmark A, Bland B, Hjelm J. Two-and three-dimensional maturation reaction norms for the eastern Baltic cod, Gadus morhua. ICES Journal of Marine Science. 2008 Dec;66(2):248–57.

48. Røjbek MC, Jacobsen C, Tomkiewicz J, Støttrup JG. Linking lipid dynamics with the reproductive cycle in Baltic cod Gadus morhua. Mar Ecol Prog Ser. 2012 Dec;471:215–34.

49. Wieland K, Horbowa K. Recent changes in peak spawning time and location of spawning of cod in the Bornholm Basin, Baltic Sea. ICES CM 1996/J:15.

50. Eero M, Vinther M, Haslob H, Huwer B, Casini M, Storr-Paulsen M, et al. Spatial management of marine resources can enhance the recovery of predators and avoid local depletion of forage fish. Conserv Lett. 2012 Dec;5(6):486–92.

51. Plambech M, Van Deurs M, Steffensen JF, Tirsgård B, Behrens JW. Excess post-hypoxic oxygen consumption in Atlantic cod Gadus morhua. J Fish Biol. 2013 Aug;83(2):396–403. doi: 10.1111/jfb.12171 23902313

52. Teschner EC, Kraus G, Neuenfeldt S, Voss R, Hinrichsen HH, Köster FW. Impact of hypoxia on consumption of Baltic cod in a multispecies stock assessment context. J Appl Ichthyol. 2010 Dec;26(6):836–42.

53. ICES Report of the Study Group on Spatial Analysis for the Baltic Sea (SGSPATIAL), 4–6 November 2014, Gothenburg, Sweden. ICES CM 2014/SSGRSP:08. 49.

54. ICES Report of the Benchmark Workshop on Baltic Cod Stocks (WKBALTCOD), 2–6 March 2015, Rostock, Germany. ICES CM 2015/ACOM:35. 172.

55. Mehrdana F, Bahlool QZ, Skov J, Marana MH, Sindberg D, Mundeling M, et al. Occurrence of zoonotic nematodes Pseudoterranova decipiens, Contracaecum osculatum and Anisakis simplex in cod (Gadus morhua) from the Baltic Sea. Vet Parasitol. 2014 Oct;205(3–4):581–7. doi: 10.1016/j.vetpar.2014.08.027 25224792

56. Svedäng H, Hornborg S. Selective fishing induces density-dependent growth. Nat Commun. 2014 Jun;5:4152. doi: 10.1038/ncomms5152 24920387

57. Andersen KH, Farnsworth KD, Thygesen UH, Beyer JE. The evolutionary pressure from fishing on size at maturation of Baltic cod. Ecol Modell. 2007 Feb;204(1–2):246–52.

58. Bagge O, Thurow F, Steffensen E, Bay J. The Baltic cod. Dana. 1994;10:1–28.

59. Nissling A, Solemdal P, Svensson M, Westin L. Survival, activity and feeding ability of Baltic cod (Gadus morhua) yolk-sac larvae at different salinities. J Fish Biol. 1994 Sep;45(3):435–45.

60. Hinrichsen HH, von Dewitz B, Dierking J, Haslob H, Makarchouk A, Petereit C, et al. Oxygen depletion in coastal seas and the effective spawning stock biomass of an exploited fish species. R Soc Open Sci. 2016 Jan;3(1):150338. doi: 10.1098/rsos.150338 26909164

61. Köster FW, Schnack D. The role of predation on early life stages of cod in the Baltic. Dana. 1994;10:179–201.

62. Åkerman G, Tjärnlund U, Broman D, Näf C, Westin L, Balk L. Comparison of reproductive success of cod, Gadus morhua, from the Barents Sea and Baltic Sea. Mar Environ Res. 1996 Jun 1;42(1–4):139–44.

63. Buchmann K, Kania P. Emerging Pseudoterranova decipiens (Krabbe, 1878) problems in Baltic cod, Gadus morhua L., associated with grey seal colonization of spawning grounds. J Fish Dis. 2012 Nov;35(11):861–6. doi: 10.1111/j.1365-2761.2012.01409.x 22817526

64. Nadolna K, Podolska M. Anisakid larvae in the liver of cod (Gadus morhua) L. from the southern Baltic Sea. J Helminthol. 2014 Jun;88(2):237–46. doi: 10.1017/S0022149X13000096 23452650

65. Zuo S, Kania PW, Mehrdana F, Marana MH, Buchmann K. Contracaecum osculatum and other anisakid nematodes in grey seals and cod in the Baltic Sea: molecular and ecological links. J Helminthol. 2018 Jan;92(1):81–9. doi: 10.1017/S0022149X17000025 28124629

66. Hansson T. Comparison of two measures of missing cofactor in cofactor-dependent enzymes: Proportion versus relative increase. Open Biomarkers Journal. 2012;5:16–21.

67. Torniainen J, Vuorinen PJ, Jones RI, Keinänen M, Palm S, Vuori KA, et al. Migratory connectivity of two Baltic Sea salmon populations: retrospective analysis using stable isotopes of scales. ICES J Mar Sci. 2013 Sep;71(2):336–44.

68. Mahé K, Schwab P, Hiscock C, Cossitt G, Briand D, Goraguer H. Age determination of Atlantic cod (Gadus morhua): 2012 Workshop between Canada and France on cod otoliths [Internet]. Saint Pierre et Miquelon: the French Ministry of the Overseas and the territorial Council; 2012. [cited 2019 April 17]. http://citeseerx.ist.psu.edu/viewdoc/download?doi=10.1.1.1015.3750%20rep=rep1%20type=pdf

69. Tate JR, Nixon PF. Measurement of Michaelis constant for human erythrocyte transketolase and thiamin diphosphate. Anal Biochem. 1987 Jan;160(1):78–87. doi: 10.1016/0003-2697(87)90616-6 3565758

70. Lowry OH, Rosenbrough NJ, Farr AL, Randall RJ. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 May;193(1):265–75. 14907713

71. Brown SB, Honeyfield DC, Vandenbyllaardt L. Thiamine analysis in fish tissues. Am Fish Soc Symp. 1998 Jan;21:73–81.

72. Kankaanpää H, Vuorinen PJ, Sipiä V, Keinänen M. Acute effects and bioaccumulation of nodularin in sea trout (Salmo trutta m. trutta L.) exposed orally to Nodularia spumigena under laboratory conditions. Aquat Toxicol. 2002 Mar;61(3–4):155–68. doi: 10.1016/s0166-445x(02)00054-1 12359387

73. Mörner T, Hansson T, Carlsson L, Berg AL, Muñoz YR, Gustavsson H, et al. Thiamine deficiency impairs common eider (Somateria mollissima) reproduction in the field. Sci Rep. 2017 Oct;7(1):14451. doi: 10.1038/s41598-017-13884-1 29089512

74. Hansson T, Thain JE, Martínez-Gómez C, Hylland K, Gubbins MJ, Balk L. Supporting variables for biological effects measurements in fish and blue mussel. ICES Techniques in Marine Environmental Science. 2017 Aug;60.

75. Kraus G, Müller A, Trella K, Köuster FW. Fecundity of Baltic cod: temporal and spatial variation. J Fish Biol. 2000 Jun;56(6):1327–41.

76. Reeves SA. A simulation study of the implications of age-reading errors for stock assessment and management advice. ICES Journal of Marine Science. 2003 Mar;60(2):314–28.

77. Araya M, Cubillos LA, Guzmán M, Peñailillo J, Sepúlveda A. Evidence of a relationship between age and otolith weight in the Chilean jack mackerel, Trachurus symmetricus murphyi (Nichols). Fish Res. 2001 Apr;51(1):17–26.

78. Fletcher WJ. A test of the relationship between otolith weight and age for the pilchard Sardinops neopilchardus. Can J Fish Aquat Sci. 1991 Jan;48(1):35–8.

79. Lepak JM, Cathcart CN, Hooten MB. Otolith mass as a predictor of age in kokanee salmon (Oncorhynchus nerka) from four Colorado reservoirs. Can J Fish Aquat Sci. 2012 Sep;69(10):1569–75.

80. Lou DC, Mapstone BD, Russ GR, Davies CR, Begg GA. Using otolith weight-age relationships to predict age-based metrics of coral reef fish populations at different spatial scales. Fish Res. 2005 Mar;71(3):279–94.

81. Pilling GM, Grandcourt EM, Kirkwood GP. The utility of otolith weight as a predictor of age in the emperor Lethrinus mahsena and other tropical fish species. Fish Res. 2003 Feb;60(2–3):493–506.

82. Cardinale M, Arrhenius F, Johnsson B. Potential use of otolith weight for the determination of age-structure of Baltic cod (Gadus morhua) and plaice (Pleuronectes platessa). Fish Res. 2000 Apr;45(3):239–52.

83. Xu ZP, Wawrousek EF, Piatigorsky J. Transketolase haploinsufficiency reduces adipose tissue and female fertility in mice. Mol Cell Biol. 2002 Jun;22(17):6142–7. doi: 10.1128/MCB.22.17.6142-6147.2002 12167708

84. Blair PV, Kobayashi R, Edwards HM, Shay NF, Baker DH, Harris RA. Dietary thiamin level influences levels of its diphosphate form and thiamin-dependent enzymic activities of rat liver. J Nutr. 1999 Mar;129(3):641–8. doi: 10.1093/jn/129.3.641 10082768

85. Jeyasingham MD, Pratt OE, Thomson AD, Shaw GK. Reduced stability of rat brain transketolase after conversion to the apo form. J Neurochem. 1986 Jul;47(1):278–81. doi: 10.1111/j.1471-4159.1986.tb02859.x 3711903

86. Trebukhina RV, Ostrovsky YM, Petushok VG, Velichko MG, Tumanov VN. Effect of thiamin deprivation on thiamin metabolism in mice. J Nutr. 1981 Mar;111(3):505–13. doi: 10.1093/jn/111.3.505 6110711

87. Harata N, Iwasaki Y, Ohara Y. Reappraisal of regional thiamine content in the central nervous system of the normal and thiamine-deficient mice. Metab Brain Dis. 1993 Mar;8(1):45–59. doi: 10.1007/bf01000529 8492784

88. Tomiyasu K, Inomata K. Enzyme-cytochemical study of small ganglion cells in experimental thiamine deficiency: Concerning the pain mechanism. Acta Neuropathol. 1991 Mar;81(4):396–400. doi: 10.1007/bf00293460 1851363

89. Rindi G, De Giuseppe L, Ventura U. Distribution and phosphorylation of oxy-thiamine in rat tissues. J Nutr. 1963 Oct;81(2):147–54.

90. Batifoulier F, Verny MA, Besson C, Demigne C, Remesy C. Determination of thiamine and its phosphate esters in rat tissues analyzed as thiochromes on a RP-amide C16 column. J Chromatogr B. 2005 Feb;816(1–2):67–72.

91. Bavington D. Managed annihilation: an unnatural history of the Newfoundland cod collapse. Vancouver: UBC press; 2011.

92. Fisheries and Oceans Canada (DFO). Recovery potential assessment for the Newfoundland and Labrador Designatable Unit (NAFO Divs. 2GHJ, 3KLNO) of Atlantic Cod (Gadus morhua). DFO Can Sci Advis Sec Sci Advis Rep. 2011/037

93. Olsen EM, Heino M, Lilly GR, Morgan MJ, Brattey J, Ernande B, et al. Maturation trends indicative of rapid evolution preceded the collapse of northern cod. Nature. 2004 Apr;428(6986):932. doi: 10.1038/nature02430 15118724

94. Swain DP. Life-history evolution and elevated natural mortality in a population of Atlantic cod (Gadus morhua). Evol Appl. 2011 Jan;4(1):18–29. doi: 10.1111/j.1752-4571.2010.00128.x 25567950

95. Dutil JD, Lambert Y. Natural mortality from poor condition in Atlantic cod (Gadus morhua). Can J Fish Aquat Sci. 2000 Apr;57(4):826–36.

96. Lambert Y, Dutil JD. Condition and energy reserves of Atlantic cod (Gadus morhua) during the collapse of the northern Gulf of St. Lawrence stock. Can J Fish Aquat Sci. 1997 Oct;54(10):2388–400.

97. Rideout RM, Morgan MJ, Lilly GR. Variation in the frequency of skipped spawning in Atlantic cod (Gadus morhua) off Newfoundland and Labrador. ICES Journal of Marine Science. 2006 Jan;63(6):1101–10.

98. Bishop CA, Baird JW. Spatial and temporal variability in condition factors of Divisions 2J and 3KL cod (Gadus morhua). NAFO Sci Coun Stud. 1994;21:105–13.

99. Vallin L, Nissling A, Westin L. Potential factors influencing reproductive success of Baltic cod, Gadus morhua: a review. Ambio. 1999 Feb;28(1):92–9.

100. Fynn-Aikins K, Bowser PR, Honeyfield DC, Fitzsimons JD, Ketola HG. Effect of dietary amprolium on tissue thiamin and Cayuga syndrome in Atlantic salmon. Trans Am Fish Soc. 1998 Sep;127(5):747–57.

101. Prasad R, Rao YVBG, Mehta K, Subrahmanyam D. Effect of thiamine deficiency on the filarial infection of albino rats with Litomosoides carinii. Int J Parasitol. 1980;10(2):93–6. doi: 10.1016/0020-7519(80)90017-x 7372401

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Článek Transcriptional Differences in Peanut (Arachis hypogaea L.) Seeds at the Freshly Harvested, After-ripening and Newly Germinated Seed Stages: Insights into the Regulatory Networks of Seed Dormancy Release and Germination
Článek Identifying maintenance hosts for infection with Dichelobacter nodosus in free-ranging wild ruminants in Switzerland: A prevalence study
Článek Model order reduction for left ventricular mechanics via congruency training
Článek Production, purification and evaluation of biodegradation potential of PHB depolymerase of Stenotrophomonas sp. RZS7
Článek The impact of a wireless audio system on communication in robotic-assisted laparoscopic surgery: A prospective controlled trial
Článek Seroprevalence of viral and vector-borne bacterial pathogens in domestic dogs (Canis familiaris) in northern Botswana
Článek Musical expertise generalizes to superior temporal scaling in a Morse code tapping task
Článek Cross-cultural adaptation and psychometric evaluation of the Yoruba version of Oswestry disability index
Článek Post-transcriptional regulation of Rad51c by miR-222 contributes cellular transformation
Článek Can scientists fill the science journalism void? Online public engagement with science stories authored by scientists
Článek Retention and predictors of attrition among patients who started antiretroviral therapy in Zimbabwe’s national antiretroviral therapy programme between 2012 and 2015
Článek Prognostics for pain in osteoarthritis: Do clinical measures predict pain after total joint replacement?
Článek Effects of Transcranial Direct Current Stimulation on GABA and Glx in Children: A pilot study
Článek Evaluation of rice wild relatives as a source of traits for adaptation to iron toxicity and enhanced grain quality
Článek Brief communication: Long-term absence of Langerhans cells alters the gene expression profile of keratinocytes and dendritic epidermal T cells
Článek APOBEC3B reporter myeloma cell lines identify DNA damage response pathways leading to APOBEC3B expression
Článek Morphological diversity within a core collection of subterranean clover (Trifolium subterraneum L.): Lessons in pasture adaptation from the wild
Článek Feasibility of real-time in vivo 89Zr-DFO-labeled CAR T-cell trafficking using PET imaging
Článek Repository-based plasmid design
Článek A new method of recording from the giant fiber of Drosophila melanogaster shows that the strength of its auditory inputs remains constant with age
Článek Aberrant cervical innate immunity predicts onset of dysbiosis and sexually transmitted infections in women of reproductive age
Článek Safe mobility, socioeconomic inequalities, and aging: A 12-year multilevel interrupted time-series analysis of road traffic death rates in a Latin American country
Článek THAP11F80L cobalamin disorder-associated mutation reveals normal and pathogenic THAP11 functions in gene expression and cell proliferation
Článek Lesion of striatal patches disrupts habitual behaviors and increases behavioral variability
Článek A clinical method for estimating the modulus of elasticity of the human cornea in vivo
Článek Patient perceived value of teleophthalmology in an urban, low income US population with diabetes
Článek Evidence in support of chromosomal sex influencing plasma based metabolome vs APOE genotype influencing brain metabolome profile in humanized APOE male and female mice
Článek Accelerated sparsity based reconstruction of compressively sensed multichannel EEG signals
Článek Microvesicles from Lactobacillus reuteri (DSM-17938) completely reproduce modulation of gut motility by bacteria in mice
Článek Dense carbon-nanotube coating scaffolds stimulate osteogenic differentiation of mesenchymal stem cells
Článek Gamma Knife radiosurgery for vestibular schwannomas: Evaluation of planning using the sphericity degree of the target volume
Článek Purification and molecular characterization of phospholipase, antigen 5 and hyaluronidases from the venom of the Asian hornet (Vespa velutina)
Článek Why are animal source foods rarely consumed by 6-23 months old children in rural communities of Northern Ethiopia? A qualitative study
Článek A study to better understand under-utilization of laboratory tests for antenatal care in Senegal
Článek Physicians’ perspectives regarding non-medical switching of prescription medications: Results of an internet e-survey
Článek Effectiveness of information technology–enabled ‘SMART Eating’ health promotion intervention: A cluster randomized controlled trial
Článek Cauda Equina Syndrome Core Outcome Set (CESCOS): An international patient and healthcare professional consensus for research studies
Článek A new species of Macrocypraea (Gastropoda, Cypraeidae) from Trindade Island, Brazil, including phenotypic differentiation from remaining congeneric species
Článek Long term conjugated linoleic acid supplementation modestly improved growth performance but induced testicular tissue apoptosis and reduced sperm quality in male rabbit
Článek A new approach to the temporal significance of house orientations in European Early Neolithic settlements
Článek Persistence of chikungunya ECSA genotype and local outbreak in an upper medium class neighborhood in Northeast Brazil
Článek In vivo elongation of thin filaments results in heart failure
Článek Disparity in depressive symptoms between heterosexual and sexual minority men in China: The role of social support
Článek Effect of classroom intervention on student food selection and plate waste: Evidence from a randomized control trial
Článek Mating strategy is determinant of adenovirus prevalence in European bats
Článek Preventing HIV and HSV-2 through knowledge and attitudes: A replication study of a multi-component community-based intervention in Zimbabwe
Článek Randomized clinical trial analyzing maintenance of peripheral venous catheters in an internal medicine unit: Heparin vs. saline
Článek Patient-related factors may influence nursing perception of sleep in the Intensive Care Unit
Článek A randomized trial of a behavioral intervention to decrease hospital length of stay by decreasing bedrest
Článek Color image segmentation using adaptive hierarchical-histogram thresholding
Článek The role of demographic history and selection in shaping genetic diversity of the Galápagos penguin (Spheniscus mendiculus)
Článek Attitudes towards animal study registries and their characteristics: An online survey of three cohorts of animal researchers
Článek Risk perception and behavioral change during epidemics: Comparing models of individual and collective learning
Článek Risk factors for third-generation cephalosporin resistant Enterobacteriaceae in gestational urine cultures: A retrospective cohort study based on centralized electronic health records
Článek Residential neighbourhood greenspace is associated with reduced risk of cardiovascular disease: A prospective cohort study
Článek Potential socioeconomic impacts from ocean acidification and climate change effects on Atlantic Canadian fisheries
Článek Prevention and control of cholera with household and community water, sanitation and hygiene (WASH) interventions: A scoping review of current international guidelines
Článek Female finches prefer courtship signals indicating male vigor and neuromuscular ability
Článek The effect of spatial position and age within an egg-clutch on embryonic development and key metabolic enzymes in two clownfish species, Amphiprion ocellaris and Amphiprion frenatus
Článek The impact of translated reminder letters and phone calls on mammography screening booking rates: Two randomised controlled trials
Článek Application of a genetic algorithm to the keyboard layout problem
Článek Design and evaluation of a laboratory-based wheelchair castor testing protocol using community data
Článek Relationship between diabetic macular edema and choroidal layer thickness
Článek Evaluation of the predictive ability of ultrasound-based assessment of breast cancer using BI-RADS natural language reporting against commercial transcriptome-based tests
Článek A Comprehensive Data Gathering Network Architecture in Large-Scale Visual Sensor Networks
Článek Recovery of health-related quality of life after burn injuries: An individual participant data meta-analysis
Článek Modeling aggressive market order placements with Hawkes factor models
Článek Role of ecology in shaping external nasal morphology in bats and implications for olfactory tracking
Článek High expression of olfactomedin-4 is correlated with chemoresistance and poor prognosis in pancreatic cancer
Článek Development and validation of a prognostic model predicting symptomatic hemorrhagic transformation in acute ischemic stroke at scale in the OHDSI network
Článek Complex patterns of cell growth in the placenta in normal pregnancy and as adaptations to maternal diet restriction
Článek Tofu intake is inversely associated with risk of breast cancer: A meta-analysis of observational studies
Článek Influence of light on the infection of Aureococcus anophagefferens CCMP 1984 by a “giant virus”
Článek Temporal ordering of input modulates connectivity formation in a developmental neuronal network model of the cortex
Článek Healthy lifestyle index and its association with hypertension among community adults in Sri Lanka: A cross-sectional study
Článek From organ to cell: Multi-level telomere length assessment in patients with idiopathic pulmonary fibrosis
Článek How do critical care staff respond to organisational challenge? A qualitative exploration into personality types and cognitive processing in critical care
Článek Effects of supplemental creatine and guanidinoacetic acid on spatial memory and the brain of weaned Yucatan miniature pigs
Článek Community-Based Health Planning and Services Plus programme in Ghana: A qualitative study with stakeholders in two Systems Learning Districts on improving the implementation of primary health care
Článek An investigation of transportation practices in an Ontario swine system using descriptive network analysis
Článek Comparison of gridded precipitation datasets for rainfall-runoff and inundation modeling in the Mekong River Basin
Článek Functional interactions in patients with hemianopia: A graph theory-based connectivity study of resting fMRI signal
Článek The effects of dual-task cognitive interference on gait and turning in Huntington’s disease
Článek Effects of Allium hookeri on gut microbiome related to growth performance in young broiler chickens
Článek Novel imaging biomarkers for mapping the impact of mild mitochondrial uncoupling in the outer retina in vivo
Článek Hyperkalemia treatment modalities: A descriptive observational study focused on medication and healthcare resource utilization
Článek Long term impact of PositiveLinks: Clinic-deployed mobile technology to improve engagement with HIV care
Článek Comparison of post-transplantation diabetes mellitus incidence and risk factors between kidney and liver transplantation patients
Článek A definition-by-example approach and visual language for activity patterns in engineering disciplines
Článek A network analysis revealed the essential and common downstream proteins related to inguinal hernia
Článek Use of conventional cardiac troponin assay for diagnosis of non-ST-elevation myocardial infarction: ‘The Ottawa Troponin Pathway’
Článek Identification and characterization of miRNAs involved in cold acclimation of zebrafish ZF4 cells
Článek Research on motion planning for an indoor spray arm based on an improved potential field method
Článek Detailed analysis of the transverse arch of hallux valgus feet with and without pain using weightbearing ultrasound imaging and precise force sensors
Článek Surrogate R-spondins for tissue-specific potentiation of Wnt Signaling
Článek Apolipoprotein-AI mimetic peptides D-4F and L-5F decrease hepatic inflammation and increase insulin sensitivity in C57BL/6 mice
Článek Treating patients with driving phobia by virtual reality exposure therapy – a pilot study
Článek Efficient processing of raster and vector data
Článek Therapeutic hypothermia after out of hospital cardiac arrest improve 1-year survival rate for selective patients
Článek Carotid plaques and neurological impairment in patients with acute cerebral infarction
Článek Deep learning based image reconstruction algorithm for limited-angle translational computed tomography
Článek Association between coffee drinking and telomere length in the Prostate, Lung, Colorectal, and Ovarian Cancer Screening Trial
Článek Hyperbaric oxygen preconditioning and the role of NADPH oxidase inhibition in postischemic acute kidney injury induced in spontaneously hypertensive rats
Článek Rad51 paralogs and the risk of unselected breast cancer: A case-control study
Článek Diagnostic differences in respiratory breathing patterns and work of breathing indices in children with Duchenne muscular dystrophy
Článek The role of narrative in collaborative reasoning and intelligence analysis: A case study
Článek Proportions of CD4 test results indicating advanced HIV disease remain consistently high at primary health care facilities across four high HIV burden countries
Článek Modelling of amino acid turnover in the horse during training and racing: A basis for developing a novel supplementation strategy
Článek Single-modal and multi-modal false arrhythmia alarm reduction using attention-based convolutional and recurrent neural networks
Článek Eye-gaze information input based on pupillary response to visual stimulus with luminance modulation
Článek Trends of litter decomposition and soil organic matter stocks across forested swamp environments of the southeastern US
Článek Post mortem evaluation of inflammation, oxidative stress, and PPARγ activation in a nonhuman primate model of cardiac sympathetic neurodegeneration
Článek Were ancient foxes far more carnivorous than recent ones?—Carnassial morphological evidence
Článek Disruption in daily eating-fasting and activity-rest cycles in Indian adolescents attending school
Článek Plasma proteome profiling of freshwater and seawater life stages of rainbow trout (Oncorhynchus mykiss)
Článek Percent amplitude of fluctuation: A simple measure for resting-state fMRI signal at single voxel level
Článek Antimicrobial activity of Asteraceae species against bacterial pathogens isolated from postmenopausal women
Článek Are changes in depressive symptoms, general health and residential area socio-economic status associated with trajectories of waist circumference and body mass index?
Článek Extracellular vesicles of U937 macrophage cell line infected with DENV-2 induce activation in endothelial cells EA.hy926
Článek Link-centric analysis of variation by demographics in mobile phone communication patterns
Článek Tobacco smoking and health-related quality of life among university students: Mediating effect of depression
Článek The Shapley value for a fair division of group discounts for coordinating cooling loads
Článek Incidence of hospital-acquired pressure ulcers in patients with "minimal risk" according to the "Norton-MI" scale
Článek Lipoprotein(a) plasma levels are not associated with survival after acute coronary syndromes: An observational cohort study
Článek Use of Nanotrap particles for the capture and enrichment of Zika, chikungunya and dengue viruses in urine
Článek Pancreatic secretory trypsin inhibitor reduces multi-organ injury caused by gut ischemia/reperfusion in mice
Článek Biochemical characterization of Ty1 retrotransposon protease
Článek Lateral pressure equalisation as a principle for designing support surfaces to prevent deep tissue pressure ulcers
Článek The validation of the Beijing version of the Montreal Cognitive Assessment in Chinese patients undergoing hemodialysis
Článek Inflammasome expression is higher in ovarian tumors than in normal ovary
Článek HCV genotype profile in Brazil of mono-infected and HIV co-infected individuals: A survey representative of an entire country
Článek Engaging with change: Information and communication technology professionals’ perspectives on change at the mid-point in the UK/EU Brexit process
Článek Adherence to iron-folic acid supplement and associated factors among antenatal care attending pregnant mothers in governmental health institutions of Adwa town, Tigray, Ethiopia: Cross-sectional study
Článek Flower, seed, and fruit development in three Tunisian species of Polygonum: Implications for their taxonomy and evolution of distyly in Polygonaceae
Článek Development of a risk score for prediction of poor treatment outcomes among patients with multidrug-resistant tuberculosis
Článek Preclinical evaluation of AT-527, a novel guanosine nucleotide prodrug with potent, pan-genotypic activity against hepatitis C virus
Článek Aqueous extract from Mangifera indica Linn. (Anacardiaceae) leaves exerts long-term hypoglycemic effect, increases insulin sensitivity and plasma insulin levels on diabetic Wistar rats
Článek Discovery of Jogalong virus, a novel hepacivirus identified in a Culex annulirostris (Skuse) mosquito from the Kimberley region of Western Australia
Článek Clinical, cytogenetic and molecular genetic characterization of a tandem fusion translocation in a male Holstein cattle with congenital hypospadias and a ventricular septal defect
Článek Detection of Torque Teno Virus (TTV) and TTV-Like Minivirus in patients with presumed infectious endophthalmitis in India
Článek CD4 rate of increase is preferred to CD4 threshold for predicting outcomes among virologically suppressed HIV-infected adults on antiretroviral therapy
Článek Estimating the basic reproduction number of a pathogen in a single host when only a single founder successfully infects
Článek What drugs modify the risk of iatrogenic impulse-control disorders in Parkinson’s disease? A preliminary pharmacoepidemiologic study
Článek Evaluating emotional distress and health-related quality of life in patients with heart failure and their family caregivers: Testing dyadic dynamics using the Actor-Partner Interdependence Model
Článek Community- and trophic-level responses of soil nematodes to removal of a non-native tree at different stages of invasion
Článek Association of ECG parameters with late gadolinium enhancement and outcome in patients with clinical suspicion of acute or subacute myocarditis referred for CMR imaging
Článek Catchment-scale export of antibiotic resistance genes and bacteria from an agricultural watershed in central Iowa
Článek Impact of multi-drug resistant bacteria on economic and clinical outcomes of healthcare-associated infections in adults: Systematic review and meta-analysis
Článek Characterization of a universal screening approach for congenital CMV infection based on a highly-sensitive, quantitative, multiplex real-time PCR assay
Článek Proof-of-concept for a non-invasive, portable, and wireless device for cardiovascular monitoring in pediatric patients
Článek On PTV definition for glioblastoma based on fiber tracking of diffusion tensor imaging data
Článek Genes associated with body weight gain and feed intake identified by meta-analysis of the mesenteric fat from crossbred beef steers
Článek Intraoperative computed tomography imaging for dose calculation in intraoperative electron radiation therapy: Initial clinical observations
Článek Human lung epithelial BEAS-2B cells exhibit characteristics of mesenchymal stem cells
Článek Simple non-mydriatic retinal photography is feasible and demonstrates retinal microvascular dilation in Chronic Obstructive Pulmonary Disease (COPD)
Článek Maternal depressive symptoms and children’s cognitive development: Does early childcare and child’s sex matter?
Článek Evaluation of a bioengineered ACL matrix’s osteointegration with BMP-2 supplementation
Článek Psychosocial profiles of physical activity fluctuation in office employees: A latent profile analysis
Článek Prevalence and characteristics of Livestock-Associated Methicillin-Resistant Staphylococcus aureus (LA-MRSA) isolated from chicken meat in the province of Quebec, Canada
Článek Soluble AXL as a marker of disease progression and survival in melanoma
Článek Using machine learning methods to determine a typology of patients with HIV-HCV infection to be treated with antivirals
Článek Gender differences influence over insomnia in Korean population: A cross-sectional study
Článek Impact of scion/rootstock reciprocal effects on metabolomics of fruit juice and phloem sap in grafted Citrus reticulata
Článek Adapting cognitive diagnosis computerized adaptive testing item selection rules to traditional item response theory
Článek Autumn shifts in cold tolerance metabolites in overwintering adult mountain pine beetles
Článek Umbilical cord separation time, predictors and healing complications in newborns with dry care
Článek Analysis of attitudinal components towards statistics among students from different academic degrees
Článek Effects of fatigue induced by repeated-sprint on kicking accuracy and velocity in female soccer players
Článek A pre-clinical validation plan to evaluate analytical sensitivities of molecular diagnostics such as BD MAX MDR-TB, Xpert MTB/Rif Ultra and FluoroType MTB
Článek Leadership for success in transforming medical abortion policy in Canada
Článek Clinical correlates associated with the long-term response of bipolar disorder patients to lithium, valproate or lamotrigine: A retrospective study
Článek Forecasting stock prices with long-short term memory neural network based on attention mechanism
Článek On the genus Crossaster (Echinodermata: Asteroidea) and its distribution
Článek Intracellular and in vivo evaluation of imidazo[2,1-b]thiazole-5-carboxamide anti-tuberculosis compounds
Článek An integrated vitamin E-coated polymer hybrid nanoplatform: A lucrative option for an enhanced in vitro macrophage retention for an anti-hepatitis B therapeutic prospect
Článek The effect of strontium and silicon substituted hydroxyapatite electrochemical coatings on bone ingrowth and osseointegration of selective laser sintered porous metal implants
Článek Molecular prevalence of Bartonella, Babesia, and hemotropic Mycoplasma species in dogs with hemangiosarcoma from across the United States
Článek Color discrimination and gas chromatography-mass spectrometry fingerprint based on chemometrics analysis for the quality evaluation of Schizonepetae Spica
Článek Comparisons of recurrence-free survival and overall survival between microwave versus radiofrequency ablation treatment for hepatocellular carcinoma: A multiple centers retrospective cohort study with propensity score matching
Článek Oral misoprostol, low dose vaginal misoprostol, and vaginal dinoprostone for labor induction: Randomized controlled trial
Článek The association between dietary patterns before and in early pregnancy and the risk of gestational diabetes mellitus (GDM): Data from the Malaysian SECOST cohort
Článek Dynamic Extreme Aneuploidy (DEA) in the vegetable pathogen Phytophthora capsici and the potential for rapid asexual evolution
Článek Assertive, trainable and older dogs are perceived as more dominant in multi-dog households
Článek Prediction of Uropathogens by Flow Cytometry and Dip-stick Test Results of Urine Through Multivariable Logistic Regression Analysis
Článek Interleukin 6 is increased in preclinical HNSCC models of acquired cetuximab resistance, but is not required for maintenance of resistance
Článek Impact of viral disease hypophagia on pig jejunal function and integrity
Článek Molecular evidence for horizontal transmission of chelonid alphaherpesvirus 5 at green turtle (Chelonia mydas) foraging grounds in Queensland, Australia
Článek Evaluation and validation of 2D biomechanical models of the knee for radiograph-based preoperative planning in total knee arthroplasty
Článek Soil-Transmitted Helminth infections reduction in Bhutan: A report of 29 years of deworming
Článek cagA gene EPIYA motif genetic characterization from Colombian Helicobacter pylori isolates: Standardization of a molecular test for rapid clinical laboratory detection
Článek Spectral characteristics of urine from patients with end-stage kidney disease analyzed using Raman Chemometric Urinalysis (Rametrix)
Článek Fast quantitative time lapse displacement imaging of endothelial cell invasion
Článek Two novel mutations in MSX1 causing oligodontia
Článek Dome-shaped macula in children and adolescents
Článek Targeted transcriptomic study of the implication of central metabolic pathways in mannosylerythritol lipids biosynthesis in Pseudozyma antarctica T-34
Článek Preliminary evidences of the presence of extracellular DNA single stranded forms in soil
Článek A comparison of quality of life between patients treated with different dialysis modalities in Taiwan
Článek Comparison of Monocyte Distribution Width (MDW) and Procalcitonin for early recognition of sepsis
Článek Morphological association between the muscles and bones in the craniofacial region
Článek Transcriptome analysis of Actinidia chinensis in response to Botryosphaeria dothidea infection
Článek Comparative study on skin protection activity of polyphenol-rich extract and polysaccharide-rich extract from Sargassum vachellianum
Článek Real-world data about emotional stress, disability and need for social care in a German IBD patient cohort
Článek The regenerative compatibility: A synergy between healthy ecosystems, environmental attitudes, and restorative experiences
Článek Antenatal depression and its association with adverse birth outcomes in low and middle-income countries: A systematic review and meta-analysis
Článek Perceptions of risk and influences of choice in pregnant women with obesity. An evidence synthesis of qualitative research
Článek The role of refugee and migrant migration status on medication adherence: Mediation through illness perceptions
Článek Sexual risk classes among youth experiencing homelessness: Relation to childhood adversities, current mental symptoms, substance use, and HIV testing
Článek Effects of CK2β subunit down-regulation on Akt signalling in HK-2 renal cells
Článek Novel broad-spectrum activity-based probes to profile malarial cysteine proteases
Článek Association between opioid analgesic therapy and initiation of buprenorphine management: An analysis of prescription drug monitoring program data
Článek Effect of a community-based approach of iron and folic acid supplementation on compliance by pregnant women in Kiambu County, Kenya: A quasi-experimental study
Článek Improvement project in higher education institutions: A BPEP-based model
Článek An updated evaluation of serum sHER2, CA15.3, and CEA levels as biomarkers for the response of patients with metastatic breast cancer to trastuzumab-based therapies
Článek Genome-wide association study of metabolic syndrome in Korean populations
Článek Drug therapy problems and treatment satisfaction among ambulatory patients with epilepsy in a specialized hospital in Ethiopia
Článek Plasma kynurenines and prognosis in patients with heart failure
Článek Occurrence and distribution of anthropogenic persistent organic pollutants in coastal sediments and mud shrimps from the wetland of central Taiwan
Článek Intensified visual clutter induces increased sympathetic signalling, poorer postural control, and faster torsional eye movements during visual rotation
Článek Gut microbiota composition alterations are associated with the onset of diabetes in kidney transplant recipients
Článek Shock index and TIMI risk index as valuable prognostic tools in patients with acute coronary syndrome complicated by cardiogenic shock
Článek Merit overrules theory of mind when young children share resources with others
Článek Metabolic analysis of amino acids and vitamin B6 pathways in lymphoma survivors with cancer related chronic fatigue
Článek Immunopathogenesis of canine chronic ulcerative stomatitis
Článek Generalizing findings from a randomized controlled trial to a real-world study of the iLookOut, an online education program to improve early childhood care and education providers’ knowledge and attitudes about reporting child maltreatment
Článek When and what to test for: A cost-effectiveness analysis of febrile illness test-and-treat strategies in the era of responsible antibiotic use
Článek Comparison of effects and safety in providing controlled hypotension during surgery between dexmedetomidine and magnesium sulphate: A meta-analysis of randomized controlled trials
Článek The gene encoding the ketogenic enzyme HMGCS2 displays a unique expression during gonad development in mice
Článek Efficacy of a mitochondrion-targeting agent for reducing the level of urinary protein in rats with puromycin aminonucleoside-induced minimal-change nephrotic syndrome
Článek Association of endothelial nitric oxide synthase (NOS3) gene polymorphisms with primary open-angle glaucoma in a Saudi cohort
Článek Antitrust analysis with upward pricing pressure and cost efficiencies
Článek Natural selection contributes to food web stability
Článek Pyramiding QTLs controlling tolerance against drought, salinity, and submergence in rice through marker assisted breeding
Článek Diversity and plant growth-promoting functions of diazotrophic/N-scavenging bacteria isolated from the soils and rhizospheres of two species of Solanum
Článek Sofosbuvir-based regimen for genotype 2 HCV infected patients in Taiwan: A real world experience
Článek The virulence domain of Shigella IcsA contains a subregion with specific host cell adhesion function
Článek Sequencing artifacts derived from a library preparation method using enzymatic fragmentation
Článek Quantitative analysis of adsorption and desorption of volatile organic compounds on reusable zeolite filters using gas chromatography
Článek Quo vadis Pantanal? Expected precipitation extremes and drought dynamics from changing sea surface temperature
Článek Cloud-computing and machine learning in support of country-level land cover and ecosystem extent mapping in Liberia and Gabon
Článek The Brief Measure of Emotional Preoperative Stress (B-MEPS) as a new predictive tool for postoperative pain: A prospective observational cohort study
Článek The impact of diabetes mellitus medication on the incidence of endogenous endophthalmitis
Článek Correction: Chl1 DNA helicase and Scc2 function in chromosome condensation through cohesin deposition
Článek Clinical and pathological features of thrombotic microangiopathy influencing long-term kidney transplant outcomes
Článek Occupational exposure to particulate matter from air pollution in the outdoor workplaces in Almaty during the cold season
Článek Morphological adjustment in free-living Steinernema feltiae infective juveniles to increasing concentration of Nemafric-BL phytonematicide
Článek Key necroptotic proteins are required for Smac mimetic-mediated sensitization of cholangiocarcinoma cells to TNF-α and chemotherapeutic gemcitabine-induced necroptosis
Článek Concurrent lipidomics and proteomics on malignant plasma cells from multiple myeloma patients: Probing the lipid metabolome
Článek Retraction: SDR9C7 Promotes Lymph Node Metastases in Patients with Esophageal Squamous Cell Carcinoma
Článek Association between tuberculosis and depression on negative outcomes of tuberculosis treatment: A systematic review and meta-analysis
Článek Bioluminescent imaging of Arabidopsis thaliana using an enhanced Nano-lantern luminescence reporter system
Článek Biosynthetic pathway of indole-3-acetic acid in ectomycorrhizal fungi collected from northern Thailand
Článek Sex-specific and opposite modulatory aspects revealed by PPI network and pathway analysis of ischemic stroke in humans
Článek Control of the microsporidian parasite Nosema ceranae in honey bees (Apis mellifera) using nutraceutical and immuno-stimulatory compounds
Článek Role of donor genotype in RT-QuIC seeding activity of chronic wasting disease prions using human and bank vole substrates
Článek Oral magnesium supplementation for leg cramps in pregnancy—An observational controlled trial
Článek Health care professionals’ knowledge of commonly used sedative, analgesic and neuromuscular drugs: A single center (Rambam Health Care Campus), prospective, observational survey
Článek Campylobacter portucalensis sp. nov., a new species of Campylobacter isolated from the preputial mucosa of bulls
Článek Transgenic interleukin 11 expression causes cross-tissue fibro-inflammation and an inflammatory bowel phenotype in mice
Článek Sleep quality and sex modify the relationships between trait energy and fatigue on state energy and fatigue
Článek The role of peer, parental, and school norms in predicting adolescents’ attitudes and behaviours of majority and different minority ethnic groups in Croatia
Článek Availability, prices and affordability of selected antibiotics and medicines against non-communicable diseases in western Cameroon and northeast DR Congo
Článek The effect of mutations derived from mouse-adapted H3N2 seasonal influenza A virus to pathogenicity and host adaptation
Článek Detection of posttraumatic pneumothorax using electrical impedance tomography—An observer-blinded study in pigs with blunt chest trauma
Článek Educators’ perceptions of organisational readiness for implementation of a pre-adolescent transdisciplinary school health intervention for inter-generational outcomes
Článek Beyond the heterodimer model for mineralocorticoid and glucocorticoid receptor interactions in nuclei and at DNA
Článek The effects of sport expertise and shot results on basketball players’ action anticipation
Článek Framework and algorithms for identifying honest blocks in blockchain
Článek Exploring the impact of terminology differences in blood and organ donor decision making
Článek Platelet indices significantly correlate with liver fibrosis in HCV-infected patients
Článek The nitrate content of fresh and cooked vegetables and their health-related risks
Článek Bioreactor for mobilization of mesenchymal stem/stromal cells into scaffolds under mechanical stimulation: Preliminary results
Článek Non-gradient and genotype-dependent patterns of RSV gene expression
Článek Multiplex real-time PCR for the detection of Clavibacter michiganensis subsp. michiganensis, Pseudomonas syringae pv. tomato and pathogenic Xanthomonas species on tomato plants
Článek The 24-hour urinary cortisol in post-traumatic stress disorder: A meta-analysis
Článek Drug-eluting versus bare-metal stents for first myocardial infarction in patients with atrial fibrillation: A nationwide population-based cohort study
Článek Health-related quality of life among patients with type 2 diabetes mellitus in Eastern Province, Saudi Arabia: A cross-sectional study
Článek “I like the way I am, but I feel like I could get a little bit bigger”: Perceptions of body image among adolescents and youth living with HIV in Durban, South Africa
Článek Nanoparticle-based ‘turn-on’ scattering and post-sample fluorescence for ultrasensitive detection of water pollution in wider window
Článek Insights into the strategy of micro-environmental adaptation: Transcriptomic analysis of two alvinocaridid shrimps at a hydrothermal vent
Článek Thirty-day readmission after medical-surgical hospitalization for people who experience imprisonment in Ontario, Canada: A retrospective cohort study
Článek Hyper-spectral response and estimation model of soil degradation in Kenli County, the Yellow River Delta
Článek The association of telomere length and telomerase activity with adverse outcomes in older patients with non-ST-elevation acute coronary syndrome
Článek Construction of a high-density genetic map and fine mapping of a candidate gene locus for a novel branched-spike mutant in barley
Článek Alterations of aqueous humor Aβ levels in Aβ-infused and transgenic mouse models of Alzheimer disease
Článek Natural hybridization between Phyllagathis and Sporoxeia species produces a hybrid without reproductive organs
Článek The impact of peer attachment on prosocial behavior, emotional difficulties and conduct problems in adolescence: The mediating role of empathy
Článek Diagnostic performance of serum interferon gamma, matrix metalloproteinases, and periostin measurements for pulmonary tuberculosis in Japanese patients with pneumonia
Článek Characterization of black patina from the Tiber River embankments using Next-Generation Sequencing
Článek Problem gambling, associations with comorbid health conditions, substance use, and behavioural addictions: Opportunities for pathways to treatment
Článek Nanosheet wrapping-assisted coverslip-free imaging for looking deeper into a tissue at high resolution
Článek Validity of cerebrovascular ICD-9-CM codes in healthcare administrative databases. The Umbria Data-Value Project
Článek Torque teno virus viral load is related to age, CMV infection and HLA type but not to Alzheimer's disease
Článek Associations of cigarette smoking and burden of thoracic aortic calcification in asymptomatic individuals: A dose-response relationship
Článek Transforming assessment of speech in children with cleft palate via online crowdsourcing
Článek Human-raptor conflict in rural settlements of Colombia
Článek Assessment of peritoneal microbial features and tumor marker levels as potential diagnostic tools for ovarian cancer
Článek Deficiency syndromes in top predators associated with large-scale changes in the Baltic Sea ecosystem
Článek Perceived relative social status and cognitive load influence acceptance of unfair offers in the Ultimatum Game
Článek Hepatitis B and C virus infection among HIV patients within the public and private healthcare systems in Chile: A cross-sectional serosurvey
Článek Retraction: Oncogenic Fibulin-5 Promotes Nasopharyngeal Carcinoma Cell Metastasis through the FLJ10540/AKT Pathway and Correlates with Poor Prognosis
Článek From seed to flour: Sowing sustainability in the use of cantaloupe melon residue (Cucumis melo L. var. reticulatus)
Článek Core Scientific Dataset Model: A lightweight and portable model and file format for multi-dimensional scientific data
Článek Learning efficient haptic shape exploration with a rigid tactile sensor array
Článek Regional versus local wind speed and direction at a narrow beach with a high and steep foredune
Článek Correction: Multiple innate antibacterial immune defense elements are correlated in diverse ungulate species
Článek Correction: Strategies for achieving high sequencing accuracy for low diversity samples and avoiding sample bleeding using illumina platform
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PLOS One

2020 Číslo 1
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Aktuální možnosti diagnostiky a léčby litiáz
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Autori: MUDr. Tomáš Ürge, PhD.
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