Antiphagocytic protein 1 increases the susceptibility of Cryptococcus neoformans to amphotericin B and fluconazole
Autoři:
Muhammad Ghaffar aff001; Cody Orr aff001; Ginny Webb aff001
Působiště autorů:
Division of Natural Sciences and Engineering, University of South Carolina Upstate, Spartanburg, South Carolina, United States of America
aff001
Vyšlo v časopise:
PLoS ONE 14(12)
Kategorie:
Research Article
prolekare.web.journal.doi_sk:
https://doi.org/10.1371/journal.pone.0225701
Souhrn
Cryptococcus neoformans is a facultative intracellular pathogen responsible for the most common cause of fungal meningioencephalitis, occurring primarily in immunocompromised individuals. Antiphagocytic protein 1 (App1) is a virulence factor produced by C. neoformans that inhibits phagocytosis of the yeast by host macrophages. Treatment of cryptococcosis includes amphotericin B, fluconazole, and flucytosine. Virulence factors have been shown to affect the susceptibility of the pathogen to antifungal drugs. In this study, we aimed to examine the relationship between App1 and antifungal drugs. We found that short-term exposure to amphotericin B downregulates APP1 expression while exposure to fluconazole upregulates APP1. In addition, App1 was found to increase the susceptibility of the yeast to amphotericin B and fluconazole. This study provides evidence of an intricate relationship between App1 and antifungal drugs.
Klíčová slova:
Gene expression – Virulence factors – Fungal pathogens – Drug therapy – Cryptococcus neoformans – Phagocytosis – Antifungals – Amphotericin
Zdroje
1. Song M-H, Lee J-W, Kim MS, Yoon J-K, White TC, Floyd A, et al. A Flucytosine-Responsive Mbp1/Swi4-Like Protein, Mbs1, Plays Pleiotropic Roles in Antifungal Drug Resistance, Stress Response, and Virulence of Cryptococcus neoformans. Eukaryotic Cell. 2012;11(1):53–67. doi: 10.1128/EC.05236-11 22080454
2. Casadevall A, Perfect J. Cryptococcus neoformans. Washington DC: American Society of Microbiology. 1998.
3. Rajasingham R, Smith RM, Park BJ, Jarvis JN, Govender NP, Chiller TM, et al. Global burden of disease of HIV-associated cryptococcal meningitis: an updated analysis. Lancet Infect Dis. 2017;17(8):873–81. Epub 2017/05/05. doi: 10.1016/S1473-3099(17)30243-8 28483415
4. Chayakulkeeree M, Perfect JR. Cryptococcosis. Infect Dis Clin N Am. 2006;20:507–44.
5. Perfect JR, Dismukes WE, Dromer F, Goldman DL, Graybill JR, Hamill RJ, et al. Clinical Practice Guidelines for the Management of Cryptococcal Disease: 2010 Update by the Infectious Diseases Society of America. Clinical Infectious Diseases. 2010;50(3):291–322. doi: 10.1086/649858 20047480
6. Voelz K, Lammas DA, May RC. Cytokine signaling regulates the outcome of intracellular macrophage parasitism by Cryptococcus neoformans. Infection and Immunity. 2009;77(8):3450–7. doi: 10.1128/IAI.00297-09 19487474
7. Bicanic T, Wood R, Meintjes G, Rebe K, Brouwer A, Loyse A, et al. High-dose amphotericin B with flucytosine for the treatment of cryptococcal meningitis in HIV-infected patients: a randomized trial. Clin Infect Dis. 2008;47(1):123–30. doi: 10.1086/588792 18505387.
8. Peng CA, Gaertner AAE, Henriquez SA, Fang D, Colon-Reyes RJ, Brumaghim JL, et al. Fluconazole induces ROS in Cryptococcus neoformans and contributes to DNA damage in vitro. PLoS One. 2018;13(12):e0208471. Epub 2018/12/07. doi: 10.1371/journal.pone.0208471 30532246
9. Odds FC, Brown AJ, Gow NA. Antifungal agents: mechanisms of action. Trends Microbiol. 2003;11(6):272–9. doi: 10.1016/s0966-842x(03)00117-3 12823944.
10. Carrillo-Muñoz AJ, Giusiano G, Ezkurra PA, Quindós G. Antifungal agents: mode of action in yeast cells. Rev Esp Quimioter. 2006;19(2):130–9. 16964330.
11. Stano P, Williams V, Villani M, Cymbalyuk ES, Qureshi A, Huang Y, et al. App1: An Antiphagocytic Protein That Binds to Complement Receptors 3 and 2. The Journal of Immunology. 2009;182(1):84–91. doi: 10.4049/jimmunol.182.1.84 19109138
12. Luberto C, Martinez-Mariño B, Taraskiewicz D, Bolaños B, Chitano P, Toffaletti DL, et al. Identification of App1 as a regulator of phagocytosis and virulence of Cryptococcus neoformans. J Clin Invest. 2003;112(7):1080–94. doi: 10.1172/JCI18309 14523045
13. Mare L, Iatta R, Montagna MT, Luberto C, Del Poeta M. APP1 transcription is regulated by inositol-phosphorylceramide synthase 1-diacylglycerol pathway and is controlled by ATF2 transcription factor in Cryptococcus neoformans. J Biol Chem. 2005;280(43):36055–64. doi: 10.1074/jbc.M507285200 16129666.
14. Tommasino N, Villani M, Qureshi A, Henry J, Luberto C, Del Poeta M. Atf2 transcription factor binds to the APP1 promoter in Cryptococcus neoformans: stimulatory effect of diacylglycerol. Eukaryot Cell. 2008;7(2):294–301. doi: 10.1128/EC.00315-07 18083832
15. Williams V, Poeta M. Role of glucose in the expression of Cryptococcus neoformans antiphagoctyic protein 1, APP1. Eukaryotic cell. 2011;10(3):293–301. doi: 10.1128/EC.00252-10 21239626
16. Florio AR, Ferrari S, De Carolis E, Torelli R, Fadda G, Sanguinetti M, et al. Genome-wide expression profiling of the response to short-term exposure to fluconazole in Cryptococcus neoformans serotype A. BMC Microbiology. 2011;11.
17. Bang S, Kwon H, Hwang HS, Park KD, Kim SU, Bahn YS. 9-O-butyl-13-(4-isopropylbenzyl)berberine, KR-72, is a potent antifungal agent that inhibits the growth of Cryptococcus neoformans by regulating gene expression. PLoS One. 2014;9(10):e109863. Epub 2014/10/10. doi: 10.1371/journal.pone.0109863 25302492
18. van Duin D, Casadevall A, Nosanchuk JD. Melanization of Cryptococcus neoformans and Histoplasma capsulatum reduces their susceptibilities to amphotericin B and caspofungin. Antimicrob Agents Chemother. 2002;46(11):3394–400. doi: 10.1128/AAC.46.11.3394-3400.2002 12384341
19. Wang Y, Casadevall A. Growth of Cryptococcus neoformans in presence of L-dopa decreases its susceptibility to amphotericin B. Antimicrob Agents Chemother. 1994;38(11):2648–50. doi: 10.1128/aac.38.11.2648 7872761
20. Ikeda R, Sugita T, Jacobson ES, Shinoda T. Effects of melanin upon susceptibility of Cryptococcus to antifungals. Microbiol Immunol. 2003;47(4):271–7. doi: 10.1111/j.1348-0421.2003.tb03395.x 12801064.
21. Zaragoza O, Mihu C, Casadevall A, Nosanchuk JD. Effect of amphotericin B on capsule and cell size in Cryptococcus neoformans during murine infection. Antimicrob Agents Chemother. 2005;49(10):4358–61. doi: 10.1128/AAC.49.10.4358-4361.2005 16189121
22. Chang M, Sionov E, Khanal Lamichhane A, Kwon-Chung KJ, Chang YC. Roles of Three Cryptococcus neoformans and Cryptococcus gattii Efflux Pump-Coding Genes in Response to Drug Treatment. Antimicrob Agents Chemother. 2018;62(4). Epub 2018/03/27. doi: 10.1128/AAC.01751-17 29378705
23. Sionov E, Chang YC, Garraffo HM, Dolan MA, Ghannoum MA, Kwon-Chung KJ. Identification of a Cryptococcus neoformans cytochrome P450 lanosterol 14α-demethylase (Erg11) residue critical for differential susceptibility between fluconazole/voriconazole and itraconazole/posaconazole. Antimicrob Agents Chemother. 2012;56(3):1162–9. Epub 2011/12/12. doi: 10.1128/AAC.05502-11 22155829
24. Sanguinetti M, Posteraro B, La Sorda M, Torelli R, Fiori B, Santangelo R, et al. Role of AFR1, an ABC transporter-encoding gene, in the in vivo response to fluconazole and virulence of Cryptococcus neoformans. Infect Immun. 2006;74(2):1352–9. doi: 10.1128/IAI.74.2.1352-1359.2006 16428784
25. Basso LR, Gast CE, Bruzual I, Wong B. Identification and properties of plasma membrane azole efflux pumps from the pathogenic fungi Cryptococcus gattii and Cryptococcus neoformans. J Antimicrob Chemother. 2015;70(5):1396–407. Epub 2015/01/27. doi: 10.1093/jac/dku554 25630649
26. Yang ML, Uhrig J, Vu K, Singapuri A, Dennis M, Gelli A, et al. Fluconazole Susceptibility in Cryptococcus gattii Is Dependent on the ABC Transporter Pdr11. Antimicrob Agents Chemother. 2015;60(3):1202–7. Epub 2015/12/07. doi: 10.1128/AAC.01777-15 26643330
27. Coelho C, Casadevall A. Cryptococcal therapies and drug targets: the old, the new and the promising. Cell Microbiol. 2016;18(6):792–9. Epub 2016/04/08. doi: 10.1111/cmi.12590 26990050
Článok vyšiel v časopise
PLOS One
2019 Číslo 12
- Metamizol jako analgetikum první volby: kdy, pro koho, jak a proč?
- Nejasný stín na plicích – kazuistika
- Masturbační chování žen v ČR − dotazníková studie
- Těžké menstruační krvácení může značit poruchu krevní srážlivosti. Jaký management vyšetření a léčby je v takovém případě vhodný?
- Fixní kombinace paracetamol/kodein nabízí synergické analgetické účinky
Najčítanejšie v tomto čísle
- Methylsulfonylmethane increases osteogenesis and regulates the mineralization of the matrix by transglutaminase 2 in SHED cells
- Oregano powder reduces Streptococcus and increases SCFA concentration in a mixed bacterial culture assay
- The characteristic of patulous eustachian tube patients diagnosed by the JOS diagnostic criteria
- Parametric CAD modeling for open source scientific hardware: Comparing OpenSCAD and FreeCAD Python scripts