Short-term treatment with a peroxisome proliferator-activated receptor α agonist influences plasma one-carbon metabolites and B-vitamin status in rats

English version

Autoři: Vegard Lysne ^aff001; Bodil Bjørndal ^aff002; Mari Lausund Grinna ^aff002; Øivind Midttun ^aff003; Per Magne Ueland ^aff002; Rolf Kristian Berge ^aff002; Jutta Dierkes ^aff005; Ottar Nygård ^aff001; Elin Strand ^aff002
Působiště autorů: Centre for Nutrition, Department of Clinical Science, University of Bergen, Bergen, Norway ^aff001; Department of Clinical Science, University of Bergen, Bergen, Norway ^aff002; Bevital A/S, Bergen, Norway ^aff003; Department of Heart Disease, Haukeland University Hospital, Bergen, Norway ^aff004; Centre for Nutrition, Department of Clinical Medicine, University of Bergen, Bergen, Norway ^aff005; Mohn Nutrition Research Laboratory, Centre for Nutrition, University of Bergen, Bergen, Norway ^aff006; Laboratory Medicine and Pathology, Haukeland University Hospital, Bergen, Norway ^aff007
Vyšlo v časopise: PLoS ONE 14(12)
Kategorie: Research Article
prolekare.web.journal.doi_sk: https://doi.org/10.1371/journal.pone.0226069

Souhrn

Introduction

Peroxisome proliferator-activated receptors (PPARs) have been suggested to be involved in the regulation of one-carbon metabolism. Previously we have reported effects on plasma concentrations of metabolites along these pathways as well as markers of B-vitamin status in rats following treatment with a pan-PPAR agonist. Here we aimed to investigate the effect on these metabolites after specific activation of the PPARα and PPARγ subtypes.

Methods

For a period of 12 days, Male Wistar rats (n = 20) were randomly allocated to receive treatment with the PPARα agonist WY-14.643 (n = 6), the PPARγ agonist rosiglitazone (n = 6) or placebo (n = 8). The animals were sacrificed under fasting conditions, and plasma concentration of metabolites were determined. Group differences were assessed by one-way ANOVA, and planned comparisons were performed for both active treatment groups towards the control group.

Results

Treatment with a PPARα agonist was associated with increased plasma concentrations of most biomarkers, with the most pronounced differences observed for betaine, dimethylglycine, glycine, nicotinamide, methylnicotinamide, pyridoxal and methylmalonic acid. Lower levels were observed for flavin mononucleotide. Fewer associations were observed after treatment with a PPARγ agonist, and the most notable was increased plasma serine.

Conclusion

Treatment with a PPARα agonist influenced plasma concentration of one-carbon metabolites and markers of B-vitamin status. This confirms previous findings, suggesting specific involvement of PPARα in the regulation of these metabolic pathways as well as the status of closely related B-vitamins.

Klíčová slova:

Blood plasma – Glycine – Serine – Metabolites – Cobalamins – Oxidation – Metabolic pathways – Cholines

Zdroje

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