Isolation and characterization of fowl aviadenovirus serotype 11 from chickens with inclusion body hepatitis in Morocco
Autoři:
Samira Abghour aff001; Khalil Zro aff003; Mohammed Mouahid aff004; Fatima Tahiri aff001; Meriam Tarta aff005; Jaouad Berrada aff002; Faouzi Kichou aff002
Působiště autorů:
Division of Pharmacy and Veterinary Inputs, ONSSA, Rabat, Morocco
aff001; Hassan 2 Institute of Agronomy and Veterinary Medicine, Rabat, Morocco
aff002; Biopharma, Rabat, Morocco
aff003; Mouahid’s Veterinary Clinic, Temara, Morocco
aff004; Faculty of Sciences and Techniques Mohammedia, Hassan II University, Casablanca, Morocco
aff005
Vyšlo v časopise:
PLoS ONE 14(12)
Kategorie:
Research Article
prolekare.web.journal.doi_sk:
https://doi.org/10.1371/journal.pone.0227004
Souhrn
The present study was conducted in order to isolate, identify and characterize fowl aviadenovirus associated with inclusion body hepatitis (IBH) in three poultry farms (two of broiler chickens and one of breeder broiler chickens) in Morocco during 2015. Liver samples collected from affected three poultry farms were examined by histopathological examination. Tissue samples showing necrosis of hepatocytes associated with basophilic intranuclear inclusion bodies were homogenized and submitted to FAdV isolation in chicken embryo fibroblast (CEF) cell cultures and in SPF embryonated eggs. The cytopathic effect (CPE) was observed in the second passage with swelling and rounding of infected cells. The inoculated embryos were hemorrhagic and showed hepatitis with the presence of basophilic intra-nuclear inclusion bodies within hepatocytes. The presence of the virus was confirmed by conventional polymerase chain reaction based on hexon gene from all investigated samples. Moreover, phylogenetic analysis of the hexon gene revealed that FAdVs isolated from different affected poultry belonged to FAdV 11 serotype of the D genotype group. This work is the first isolation in cell culture and SPF embryonated eggs of FAdV from Moroccan broilers and breeder broiler chickens with IBH.
Klíčová slova:
Embryos – Polymerase chain reaction – Cell cultures – DNA sequence analysis – Livestock – Poultry – Chickens – Hepatocytes
Zdroje
1. McFerran JB, Adair BM. Group I adenovirus infection. In: Saif YM, Barnes HJ, Glisson JR, Fadly AM, McDougald LRet al, editors. Diseases of poultry, 11th Ed. Ames: Iowa State Press. 2003; pp. 214–227.
2. Benko M, Harrach B, Russel WC. Family Adenoviridae. In: van Regenmortel MHV et al, editors. Virus Taxonomy. Classification and nomenclature of viruses. Seventh Report of the International Committee on Taxonomy of Viruses: Academic Press, San Diego; 2000. pp. 227–238.
3. Hess M. Detection and differentiation of avian adenoviruses: A review. Avian Pathol. 2000; 29: 195–206. doi: 10.1080/03079450050045440 19184805
4. Guy JS, Schaeffer JL, Barnes HJ. Inclusion-body hepatitis in day-old turkeys. Avian Dis. 1988; 32: 587–590. 2848488
5. Hess M, Prusas C, Monreal G. Growth analysis of adenoviruses isolated from pigeons in chicken cells and serological characterization of the isolates. Avian Pathol. 1998; 27: 196–199. doi: 10.1080/03079459808419323 18483986
6. Mc Ferran JB, McCracken RM, Connor TJ, Evans RT. Isolation of viruses from clinical outbreaks of inclusion body hepatitis. Avian Pathol. 1976; 5: 315–324. doi: 10.1080/03079457608418201 18777361
7. Takase K, Yoshinaga N, Egashira T, Uchimura T, Yamamoto M. Avian adenovirus isolated from pigeons affected with inclusion body hepatitis. Japanese Journal of Veterinary Science. 1990; 52: 207–215. doi: 10.1292/jvms1939.52.207 2161475
8. Riddell C. Viral hepatitis in domestic gees in Saskatchewan. Avian Dis. 1984; 28: 774–782. 6091609
9. Capua I, Liberti L, Gough RE, Casaccia C, Asdrubali G. Isolation and characterization of an adenovirus associated with inclusion body hepatitis in psittacine birds. Avian Pathol. 1995; 24: 717–722. doi: 10.1080/03079459508419110 18645827
10. Droual R, Woolcock PR, Nordhausen RW, Fitzgerald SD. Inclusion body hepatitis and hemorrhagic enteritis in two African grey parrots (Psittacus erithacus) associated with adeno-virus. J. Vet. Diagn. Invest. 1995; 7: 150–154. doi: 10.1177/104063879500700125 7779952
11. Hess M. Aviadenovirus infections. In: Swayne D, Glisson JR, McDougald LR, Nolan LK, Suarez DL, V, editors. Diseases of poultry, 13th ed. Ames: Wiley-Blackwell. 2013; pp. 290–300.
12. Schachner A, Marek A, Grafl B, Hess M. Detailed molecular analyses of the hexon loop1 and fibers of fowl aviadenoviruses reveal new insights into the antigenic relationship and confirm that specific genotype are involved in fied outbreaks of inclusion body hepatitis. Vet Microbiol. 2016; 186: 13–20. doi: 10.1016/j.vetmic.2016.02.008 27016752
13. McFerran JB, Smyth JA. Avian adenoviruses. Rev.—Off. Int. Epizoot. 2000; 589–601. 10935281
14. Grimes TM, King DJ, Kleven SH, Fletcher OJ. Involvement of type-8 avian adenovirus in the etiology of inclusion body hepatitis. Avian Dis. 1977; 21, 26–38. 190994
15. Hair-Bejo M. Inclusion body hepatitis in a flock of commercial broiler chickens. J. Vet. Malaysia.2005; 17: 23–26.
16. Mase M, Mitake H, Inoue T, Imada T. Identification of group I-III avian adenovirus by PCR coupled with direct sequencing of hexon gene. J. Vet. Med. Sci. 2009; 71: 1239–1242. doi: 10.1292/jvms.71.1239 19801907
17. Soumyalekshmi S, Ajith MK, Meshram C. Isolation of fowl adenovirus in chicken embryo liver cell culture and its detection by hexon gene based PCR. Indian Journal of Scientific Research and Technology. 2014; 2(3): 33–36.
18. Xie Z, Fadl A, Girshick T, Khan M. Detection of Avian Adenovirus by Polymerase Chain Reaction. Avian Dis. 1999; 43(1): 98–105. 1999. 10216765
19. Meulemans G, Boschmans M, Berg TP, Decaesstecker M. Polymerase chain reaction combined with restriction enzyme analysis for detection and differentiation of fowl adenoviruses. Avian Pathol. 2001; 30:655–660. doi: 10.1080/03079450120092143 19184959
20. Raue R, Hess M. Hexon based PCRs combined with restriction enzyme analysis for rapid detection and differentiation of fowl adenovirus and egg drop syndrome virus. J. Virol. Methods. 1998; 73: 211–217. doi: 10.1016/s0166-0934(98)00065-2 9766892
21. Mouahid M, Bengoumi M, Kichou F. Case report of recent outbreak of inclusion body hepatitis among broilers in Morocco. Abstract. Proc. 18th World Veterinary Poultry Association Congress, Nantes, France. 2013; pp. 562.
22. Said Anas. Etude clinico-pathologique de cas d’hépatite à inclusion chez la volaille entre 2012 et 2015. Thesis. Institut Agronomique et Vétérinaire Hassan II, Rabat, Morocco. 2015.
23. Salek M, El Houadfi M. The first case and description of FAdV-11 causing high mortalities in very young broiler breeders in Morocco. Abstract. Proc. 19th World Veterinary Poultry Association Congress, Edinburgh, UK. 2017.
24. Redondo H, Sarabia FJ, Ait Tahala M, Bensassi Y, Gil I, Elbachir Erraji, et al. Characterization of strain of fowl adenoviruses circulating in Morocco. Poult Sci. 2018; 0:1–6.
25. Schachner A, Matos M, Grafl B, Hess M. Fowl adenovirus-induced diseases and strategies for their control- a review on the current global situation. Avian pathol. 2018; 47(2): 111–126. doi: 10.1080/03079457.2017.1385724 28950714
26. Mendelson C, Nothelfer HB, Monreal G. Identification and characterization of an avian adenovirus isolated from a spiking mortality syndrome field outbreak in broilers on the Delmarva Peninsula, USA. Avian Pathol. 1995; 24:693–706. doi: 10.1080/03079459508419108 18645825
27. Mittal D, Jindal N, Tiwari AK, Khkhar RS. Characterization of fowl adenoviruses associated with hydropericardium syndrome and inclusion body hepatitis in broiler chickens. Virusdisease. 2014; 25:114–119. doi: 10.1007/s13337-013-0183-7 24426318
28. Ojkic D, Martin E, Swinton J, Vaillancourt JP, Boulianne M, Gomis S. Genotyping of canadian isolates of fowl adenoviruses. Avian Pathol. 2008; 37:95–100. doi: 10.1080/03079450701805324 18202956
29. Kajan GL, Kecskemeti S, Harrach B, Benko M. Molecular typing of fowl adenoviruses, isolated in Hungary recently, reveals high diversity. Vet Microbiology. 2013; 167: 357–363.
30. Choi KS, Kye SJ, Kim JY, Jeon WJ, Lee EK, Park KY, et al. Epidemiological investigation of outbreaks of fowl adenovirus infection in commercial chickens in Korea. Poult. Sci. 2012; 91:2502–2506. doi: 10.3382/ps.2012-02296 22991534
31. Shaib H, Ramadan N, Mahmoud G, Nassif G, Chedid S. Outbreak of inclusion body hepatitis causing Adenovirus in Lebanese Broiler flocks. EC Microbiology. 2017; 13.3:92–101.
32. Oliver-Ferrando S, Dolz R, Calderón C, Valle R, Rivas R, Pérez M, et al. Epidemiological and pathological investigation of fowl aviadenovirus serotypes 8b and 11 isolated from chickens with inclusion body hepatitis in Spain (2011–2013). Avian Pathol. 2017; 46(2): 157–165. doi: 10.1080/03079457.2016.1232477 27928940
33. Kaur G, Maiti NK, Oberoi M. Biological and immunological characterization of fowl adenovirus-4 isolates from inclusion body hepatitis-hydropericardium syndrome. Indian. J. Anim. Sci. 2003; 73(1):51–52.
34. Kim JN, Byun SH, Kim MJ, Kim Jj, Sung HW, Mo IP. Outbreaks of hydropericardium syndrome and molec-ular characterization of Korean fowl adenoviral isolates. Avian Dis. 2008; 52:526–530. doi: 10.1637/8178-112207-Case 18939647
35. Kumar V, Kumar R, Chandra R, Bhatt P, Dhama K. Outbreaks of Inclusion Body Hepatitis (IBH) in Chickens; Pathological Studies and Isolation of Fowl Adenovirus. Advances in Animal and Veterinary Sciences. 2013; 1(3(S)):21–24.
36. Almemnesc W, Hair-Bejo M, Aini I, Omar AR. Pathogenicity of fowl adenovirus in specific pathogen free chicken embryos. J Comp Pathol. 2012; 146: 223–229. doi: 10.1016/j.jcpa.2011.05.001 21705014
37. Matos M, Grafl B, Liebhart D, Hess M. The outcome of experimentally induced inclusion body hepatitis (IBH) by fowl aviadenoviruses (FAdVs) is crucially influenced by the genetic background of the host. J. Vet. Res. 2016; 47: 69.
38. Steer-Cope P, Sandy J, O’Rourke D, Scott P, Browwning G, Noormohammadi A. Chronologic analysis of gross and histologic lesions induced by field strains of FAdV-1, FAdV-8b, FAdV-11 in six week old chickens. Avian Dis. 2017; 61(4): 512–519. doi: 10.1637/11718-072317-Reg.1 29337616
39. Domanska-Blicharz K, Tomczyk G, Smietanka K, Kozaczynski W, Minta Z. Molecular characterization of fowl adenoviruses isolated from chickens with gizzard erosions. Poult Sci. 2011; 90:983–989. doi: 10.3382/ps.2010-01214 21489943
40. Niczyporuk JS. Molecular characterization of fowl adenovirus type 7 isolated from poultry associated with inclusion body hepatitis in Poland. Arch. Virol. 2017; 162:1325–1333. doi: 10.1007/s00705-017-3240-5 28160143
41. Niczyporuk JS, Wozniakowski G, Samorek-Salamonowicz E. Application of cross-priming amplification (CPA) for detection of fowl adenovirus strain. Arch. Virol. 2015; 160:1005–1013. doi: 10.1007/s00705-015-2355-9 25655263
42. Gulhane AB, Deshpande AA, Gogoi S, Kumar P. Isolation and characterization of different fowl adenovirus types associated with inclusion body hepatitis in broiler chickens of India. J Pure Appl Microbio. 2016; 10(1): 417–423.
43. Absalon AE, Morales-Garzon A, Vera-Hernandez PF, Cortés-Espinosa DV, Uribe-Ochoa SM, Garcia LJ, et al. Complete genome sequence of a non-pathogenic strain of fowl adenovirus serotyoe 11: Minimal genomic differences between pathogenic and non-pathogenic viruses. J Virol. 2016; 501: 63–69.
44. Asthana M, Singh VK, Kumar R, Chandra R. Isolation, cloning and silico study of hexon gene of fowl adenovirus 4 isolates associated with hydro pericardium syndrome in domestic fowl. J Proteomics Bioinform. 2011; 4(9): 190–195.
45. Dahiya S, Srivastava RN, Hess M, Gulati BR. Fowl Adenovirus serotype4 Associated with Outbreaks of Infectious Hydropericardium in Haryana, India. Avian Dis. 2002; 46:230–233. doi: 10.1637/0005-2086(2002)046[0230:FASAWO]2.0.CO;2 11922341
46. Grafl B, Liebhart D, Gunes A, Wernsdorf P, Aigner F, Bachmeier J, et al. Quantity of virulent fowl adenovirus serotype 1 correlates with clinical signs, macroscopical and pathohistological lesions in gizzards following experimental induction of gizzard erosion in broilers. Vet Res. 2013; 44:38. doi: 10.1186/1297-9716-44-38 23705834
47. Hong-Su P, II-Soo L, Sang-Kyu K, Toh-Kyung K, Sang-Geon Y. Isolation and characterization of fowl adenovirus serotype 4 from chicken with hydropericardium syndrome in korea. Korean. J. Vet. Res. 2001; 51(3): 209–216.
48. Radwan MM, El-Deeb AH, Mousa MR, El-Sanousi AA, Shalaby MA. First report of fowl adenovirus 8a from commercial broiler chickens in Egypt: molecular characterization and pathogenicity. Poult Sci. 2018; 0:1–8.
49. Mase M, Nakamura K, Minami F. Fowl adenoviruses isolated from chickens with inclusion body hepatitis in Japan, 2009–2010. J. Vet. Med. Sci. 2012; 74(8):1087–1089. doi: 10.1292/jvms.11-0443 22516693
50. Niczyporuk JS, Samorek-Salamonowicz E, Czekaj H. Analysis of adenovirus strains isolated from poultry in Poland. Bull Vet Inst Pulawy. 2013; 57: 305–310.
51. Okuda Y, Ono M, Shibata I, Sato S. Pathogenicity of serotype 8 fowl adenovirus isolated from gizzard erosions of slaughtered broiler chickens. J. Vet. Med. Sci. 2004; 66:1561–1566. doi: 10.1292/jvms.66.1561 15644608
52. Philippe C, Grgic H, Ojkic D, Nagy É. Serologic monitoring of a broiler breeder flock previously affected by inclusion body hepatitis and testing of the progeny for vertical transmission of fowl adenoviruses. Can. J. Vet. Res. 2007; 71:98–102. 17479772
53. Dar A, Gomis S, Shirley I, Mutwiri G, Brownlie R, Potter A, et al. Pathotypic and Molecular characterization of a fowl adenovirus associated with inclusion body hepatitis in Saskatchewan chickens. Avian Dis. 2012; 56:73–81. doi: 10.1637/9764-041911-Reg.1 22545531
54. Ojkic D, Krell PJ, Tuboly T, Nagy É. Characterization of fowl adenoviruses isolated in Ontario and Quebec, Canada. Can. J. Vet. Res. 2008; 72:236–241. 18505186
55. Hemida MG, Al-Hammadi M. Prevalence and molecular charactestics of fowl adenovirus serotype 4 in eastern Saudi Arabia. Turk. J. Vet. Anim. Sci. 2017; 41:506–513.
56. Ganesh K, Suryanarayana VVS, Raghavan R. Detection of fowl adenovirus associated with hydropericardium hepatitis syndrome by a polymerase chain reaction. Vet.Res.Commun. 2002; 26:73–80. doi: 10.1023/a:1013361906791 11862998
57. Rahul S, Kataria JM, Senthilkumar N, Dhama K, Uma R, Sylvester SA, et al. Polymerase chain reaction based differentiation of various fowl adeovirus serotypes causing inclusion body hepatitis-hydropericardium syndrome of poultry in india. Indian. J. Comp. Immunol. Microbiol. Infect. Dis. 2004; 25(1):1–6.
58. Mase M, Nakamura K. Phylogenetic analysis of fowl adenoviruses isolated from chickens with gizzard erosion in Japan. J. Vet. Med. Sci. 2014; 76(11):1535–1538. doi: 10.1292/jvms.14-0312 25131809
59. Zhao J, Zhong Q, Zhao Y, Hu Y X, Zhang G Z. Pathogenicity and Complete Genome Characterization of Fowl Adenoviruses Isolated from Chickens Associated with Inclusion Body Hepatitis and Hydropericardium Syndrome in China. Plos one. 2015; 10(7): e0133073. doi: 10.1371/journal.pone.0133073 26167857
60. Ghafari PT, Boroomand Z, Rezaie A, Mayahi M, Eftekharian S. Detection and identification of avian adenovirus in broiler chickens suspected of inclusion body hepatitis in Khuzestan, Iran during 2015–2016. Iranian Journal of Veterinary Science and Technology. 2018; 9(2): 41–45.
Článok vyšiel v časopise
PLOS One
2019 Číslo 12
- Metamizol jako analgetikum první volby: kdy, pro koho, jak a proč?
- Nejasný stín na plicích – kazuistika
- Masturbační chování žen v ČR − dotazníková studie
- Těžké menstruační krvácení může značit poruchu krevní srážlivosti. Jaký management vyšetření a léčby je v takovém případě vhodný?
- Fixní kombinace paracetamol/kodein nabízí synergické analgetické účinky
Najčítanejšie v tomto čísle
- Methylsulfonylmethane increases osteogenesis and regulates the mineralization of the matrix by transglutaminase 2 in SHED cells
- Oregano powder reduces Streptococcus and increases SCFA concentration in a mixed bacterial culture assay
- The characteristic of patulous eustachian tube patients diagnosed by the JOS diagnostic criteria
- Parametric CAD modeling for open source scientific hardware: Comparing OpenSCAD and FreeCAD Python scripts