Deep brain stimulation restores the glutamatergic and GABAergic synaptic transmission and plasticity to normal levels in kindled rats

English version

Autoři: Samireh Ghafouri ^aff001; Yaghoub Fathollahi ^aff001; Saeed Semnanian ^aff001; Amir Shojaei ^aff001; Azam Asgari ^aff001; Azin Ebrahim Amini ^aff003; Javad Mirnajafi-Zadeh ^aff001
Působiště autorů: Department of Physiology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran ^aff001; Département de Neurosciences, Université de Montréal, Montréal, Canada ^aff002; Department of Biomaterial and Biomedical Engineering (IBBME), Faculty of applied sciences, University of Toronto, Toronto, Canada ^aff003; Institute for Brain Sciences and Cognition, Tarbiat Modares University, Tehran, Iran ^aff004
Vyšlo v časopise: PLoS ONE 14(11)
Kategorie: Research Article
prolekare.web.journal.doi_sk: https://doi.org/10.1371/journal.pone.0224834

Souhrn

Background

The precise effect of low frequency stimulation (LFS) as a newly postulated, anticonvulsant therapeutic approach on seizure-induced changes in synaptic transmission has not been completely determined.

Hypothesis

In this study, the LFS effect on impaired, synaptic plasticity in kindled rats was investigated.

Methods

Hippocampal kindled rats received LFS (4 trials consisting of one train of 200 monophasic square waves, 0.1 ms pulse duration, 1 Hz) on four occasions. LTP induction was evaluated using whole-cell recordings of evoked excitatory and inhibitory post-synaptic potentials (EPSPs and IPSPs respectively) in CA1 neurons in hippocampal slices. In addition, the hippocampal excitatory and inhibitory post-synaptic currents (EPSCs and IPSCs), and the gene expression of NR₂A, GluR₂ and γ₂ were evaluated.

Results

LTP induction was attenuated in excitatory and inhibitory synapses in hippocampal slices of kindled rats. When LFS was applied in kindled animals, LTP was induced in EPSPs and IPSPs. Moreover, LFS increased and decreased the threshold intensities of EPSCs and IPSCs respectively. In kindled animals, NR₂A gene expression increased, while γ₂ gene expression decreased. GluR2 gene expression did not significantly change. Applying LFS in kindled animals mitigated these changes: No significant differences were observed in NR₂A, γ₂ and GluR₂ gene expression in the kindled+LFS and control groups.

Conclusion

The application of LFS in kindled animals restored LTP induction in both EPSPs and IPSPs, and returned the threshold intensity for induction of EPSCs, IPSCs and gene expression to similar levels as controls.

Klíčová slova:

Gene expression – Neurons – Functional electrical stimulation – Synapses – Gamma-aminobutyric acid – Synaptic plasticity – Neurotransmission – Anticonvulsants

Zdroje

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