Protective effect of Platymiscium floribundum Vog. in tree extract on periodontitis inflammation in rats
Autoři:
Jordânia M. O. Freire aff001; Hellíada V. Chaves aff001; Alrieta H. Teixeira aff002; Luzia Herminia T. de Sousa aff001; Isabela Ribeiro Pinto aff001; José Jackson do N. Costa aff003; Nayara Alves de Sousa aff004; Karuza Maria A. Pereira aff005; Virgínia C. C. Girão aff005; Vanessa C. S. Ferreira aff006; João Evangelista de Ávila dos Santos aff007; Mary Anne S. Lima aff007; Antônia T. A. Pimenta aff007; Raquel de C. Montenegro aff008; Maria Elisabete A. de Moraes aff008; Vicente de P. T. Pinto aff004; Gerardo C. Filho aff004; Mirna M. Bezerra aff004
Působiště autorů:
Northeast Biotechnology Network–Ph.D. Program, Federal University of Ceará, Sobral, Ceará, Brazil
aff001; School of Dentistry, Federal University of Ceará, Sobral, Ceará, Brazil
aff002; School of Medicine, University CenterINTA–UNINTA, Sobral, Ceará, Brazil
aff003; School of Medicine, Federal University of Ceará, Sobral, Ceará, Brazil
aff004; Department of Morphology, School of Medicine, Federal University of Ceará, Fortaleza, Ceará, Brazil
aff005; Postgraduate Program in Morphofunctional Sciences, Department of Morphology, School of Medicine, Federal University of Ceará, Fortaleza, Ceará, Brazil
aff006; Department of Organic and Inorganic Chemistry, Federal University of Ceará, Fortaleza, Ceará, Brazil
aff007; Drug Research and Development Center (NPDM), Federal University of Ceará, Fortaleza, Ceará, Brazil
aff008
Vyšlo v časopise:
PLoS ONE 14(11)
Kategorie:
Research Article
prolekare.web.journal.doi_sk:
https://doi.org/10.1371/journal.pone.0223800
Souhrn
Periodontitis is an immuno-inflammatory disease, which can lead to tooth loss. This study aimed to investigate the efficacy of Platymiscium floribundum Vog., a Brazilian tree which has been used in folk medicine as an anti-inflammatory agent, in a pre-clinical trial of periodontitis in rats. Periodontitis was induced by placing a sterilized nylon (3.0) thread ligature around the cervix of the second left upper molar of the rats, which received (per os) P. floribundum extract (0.1, 1 or 10 mg/kg) or vehicle 1h before periodontitis-challenge and once daily during 11 days. Treatment with P. floribundum (10mg/kg) decreased alveolar bone loss, MPO activity nitrite/nitrate levels, oxidative stress, TNF-α, IL1-β, IL-8/CINC-1, and PGE2 gingival levels, and transcription of TNF-α, IL1-β, COX-2, iNOS, RANK, and RANKL genes, while elevated both BALP serum levels and IL-10 gingival levels. The animals did not show signs of toxicity throughout the experimental course. These findings show that P. floribundum has anti-inflammatory and anti-resorptive properties in a pre-clinical trial of periodontitis, representing an interesting biotechnological tool.
Klíčová slova:
Cytokines – Inflammation – Toxicity – Oxidative stress – Nitric oxide – Scanning electron microscopy – Alveolar bone – Periodontitis
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