Verification of hub genes in the expression profile of aortic dissection

English version

Autoři: Weitie Wang ^aff001; Qing Liu ^aff002; Yong Wang ^aff001; Hulin Piao ^aff001; Bo Li ^aff001; Zhicheng Zhu ^aff001; Dan Li ^aff001; Tiance Wang ^aff001; Rihao Xu ^aff001; Kexiang Liu ^aff001
Působiště autorů: Department of Cardiovascular Surgery, Second Hospital of Jilin University, Changchun, Jilin, China ^aff001; Graduate School of Medicine and Faculty of Medicine, University of Tokyo, Tokyo, Japan ^aff002
Vyšlo v časopise: PLoS ONE 14(11)
Kategorie: Research Article
prolekare.web.journal.doi_sk: https://doi.org/10.1371/journal.pone.0224922

Souhrn

Background

To assess the mRNA expression profile and explore the hub mRNAs and potential molecular mechanisms in the pathogenesis of human thoracic aortic dissection (TAD).

Methodology

mRNA microarray expression signatures of TAD tissues (n = 6) and non-TAD tissues (NT; n = 6) were analyzed by an Arraystar human mRNA microarray. Real-time PCR (qRT-PCR) was used to validate the results of the mRNA microarray. Bioinformatic tools, including Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathway analysis, were utilized. Protein-protein interaction (PPI) networks were constructed based on data from the STRING database. Molecular Complex Detection (MCODE) and cytoHubba analyses were used to predict the strongest hub gene and pathway.

Results

The top 10 hub genes were CDK1, CDC20, CCNB2, CCNB1, MAD2L1, AURKA, C3AR1, NCAPG, CXCL12 and ASPM, which were identified from the PPI network. Module analysis revealed that TAD was associated with the cell cycle, oocyte meiosis, the p53 signaling pathway, and progesterone-mediated oocyte maturation. The qRT-PCR results showed that the expression of all hub genes was significantly increased in TAD samples (p < 0.05). Immunostaining of Ki-67 and CDK1 showed a high proliferation state and high expression in TAD, respectively.

Conclusions

CDK1 could be used as a potential diagnostic biomarker and therapeutic target of TAD.

Klíčová slova:

Gene expression – Cell cycle and cell division – Cellular structures and organelles – Immune response – Binding analysis – Cytoskeletal proteins – Microarrays – Muscle contraction

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