Treatment of corneal endothelial damage in a rabbit model with a bioengineered graft using human decellularized corneal lamina and cultured human corneal endothelium

English version

Autoři: Francisco Arnalich-Montiel ^aff001; Adrian Moratilla ^aff002; Sherezade Fuentes-Julián ^aff002; Veronica Aparicio ^aff002; Marta Cadenas Martin ^aff002; Gary Peh ^aff003; Jodhbir S. Mehta ^aff003; Khadijah Adnan ^aff003; Laura Porrua ^aff001; Ane Pérez-Sarriegui ^aff001; Maria P. De Miguel ^aff002
Působiště autorů: Ophthalmology Department, Hospital Ramón y Cajal, Madrid, Spain ^aff001; Cell Engineering Laboratory, La Paz Hospital Research Institute, iDIPAZ, Madrid, Spain ^aff002; Singapore Eye Research Institute, Singapore, Singapore ^aff003
Vyšlo v časopise: PLoS ONE 14(11)
Kategorie: Research Article
prolekare.web.journal.doi_sk: https://doi.org/10.1371/journal.pone.0225480

Souhrn

Objective

We aimed to investigate the functionality of human decellularized stromal laminas seeded with cultured human corneal endothelial cells as a tissue engineered endothelial graft (TEEK) construct to perform endothelial keratoplasty in an animal model of corneal endothelial damage.

Methods

Engineered corneal endothelial grafts were constructed by seeding cultured human corneal endothelial cell (hCEC) suspensions onto decellularized human corneal stromal laminas with various coatings. The functionality and survival of these grafts with cultured hCECs was examined in a rabbit model of corneal endothelial damage after central descemetorhexis. Rabbits received laminas with and without hCECs (TEEK and control group, respectively).

Results

hCEC seeding over fibronectin-coated laminas provided an optimal and consistent endothelial cell count density and polygonal shape on the decellularized laminas, showing active pump fuction. Surgery was performed uneventfully as standard Descemet stripping automated endothelial keratoplasty (DSAEK). Corneal transparency gradually recovered in the TEEK group, whereas haze and edema persisted for up to 4 weeks in the controls. Histologic examination showed endothelial cells of human origin covering the posterior surface of the graft in the TEEK group.

Conclusions

Grafting of decellularized stroma carriers re-surfaced with human corneal endothelial cells ex vivo can be a readily translatable method to improve visual quality in corneal endothelial diseases.

Klíčová slova:

Eyes – Cornea – Endothelial cells – Rabbits – Corneal transplantation – Coatings – Endothelium

Zdroje

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