Genome-wide identification and characterization, phylogenetic comparison and expression profiles of SPL transcription factor family in B. juncea (Cruciferae)

English version

Autoři: Jian Gao ^aff001; Hua Peng ^aff003; Fabo Chen ^aff001; Yi Liu ^aff001; Baowei Chen ^aff001; Wenbo Li ^aff001
Působiště autorů: Department of Life Sciences and Technology, Yangtze Normal University, Fuling, Chongqing, China ^aff001; Centre for Green Development and Collaborative Innovation in Wuling Mountain Region, Yangtze Normal University, Fuling, Chongqing, China ^aff002; Sichuan Tourism College, Chengdu, Sichuan, China ^aff003
Vyšlo v časopise: PLoS ONE 14(11)
Kategorie: Research Article
prolekare.web.journal.doi_sk: https://doi.org/10.1371/journal.pone.0224704

Souhrn

SQUAMOSA promoter-binding protein-like (SPL), as plant specific transcription factors, is involved in many plant growth and development processes. However, there is less systematical study for SPL transcription factor in B. juncea (Cruciferae). Here, a total of 59 SPL genes classified into eight phylogenetic groups were identified in B. juncea, highly conserved within each ortholog were also found based on gene structure, conserved motif, as well as clustering level. In addition, clustering of SPL domain showed that two zinc finger-like structures and NLS segments were identified in almost of BjuSPLs. Analyzed of putative cis-elements for BjuSPLs demonstrated that SPL transcription factors were involved in adverse environmental changes, such as light, plant stresses and phytohormones response. Expression analysis showed that differentially expressed SPL genes were identified in flower and stem development of Cruciferae; such as BjuSPL3a-B, BjuSPL2b_B and BjuSPL2c_A were significantly expressed in flower; BjuSPL 3b_B and BjuSPL10a_A were significantly expressed in stem node (VP: vegetative period). Moreover, 28 of the 59 BjuSPLs were found involved in their posttranscriptional regulation targeted by miR156. We demonstrated that miR156 negatively regulated BjuSPL10a_A and BjuSPL3b_B to act for stem development in B. juncea.

Klíčová slova:

Phylogenetic analysis – Sequence motif analysis – Transcription factors – Protein domains – Seedlings – Introns – Protein structure comparison – Arabidopsis thaliana

Zdroje

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Genome-wide identification and characterization, phylogenetic comparison and expression profiles of SPL transcription factor family in B. juncea (Cruciferae)

Souhrn

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Zdroje

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