Occurrence and multilocus genotyping of Giardia duodenalis from post-weaned dairy calves in Sichuan province, China
Autoři:
Jiaming Dan aff001; Xueping Zhang aff001; Zhihua Ren aff001; Liqin Wang aff002; Suizhong Cao aff001; Liuhong Shen aff001; Junliang Deng aff001; Zhicai Zuo aff001; Shumin Yu aff001; Ya Wang aff001; Xiaoping Ma aff001; Haifeng Liu aff001; Ziyao Zhou aff001; Yanchun Hu aff001; Hualin Fu aff001; Changliang He aff001; Yi Geng aff001; Xiaobin Gu aff001; Guangneng Peng aff001; Zhijun Zhong aff001
Působiště autorů:
College of Veterinary Medicine, Sichuan Agricultural University, Key Laboratory of Animal Disease and Human Health of Sichuan, Chengdu, China
aff001; The Chengdu Zoo, Institute of Wild Animals, Chengdu, China
aff002
Vyšlo v časopise:
PLoS ONE 14(11)
Kategorie:
Research Article
prolekare.web.journal.doi_sk:
https://doi.org/10.1371/journal.pone.0224627
Souhrn
Giardia duodenalis is a zoonotic parasitic protist and poses a threat to human and animal health. This study investigated the occurrence of G. duodenalis infection in post-weaned calves from Sichuan province, China. Faecal samples were collected from a total of 306 post-weaned calves (3–12 months old) from 10 farms, including 4 intensive feeding farms and 6 free-ranging farms. The overall infection rate of G. duodenalis was 41.2% (126/306) based on the PCR results at any of the three genetic loci: beta-giardin (bg), triose-phosphate isomerase (tpi) and glutamate dehydrogenase (gdh) genes. Giardia duodenalis assemblages E (n = 115, 91.3%), A (n = 3, 2.4%), and A mixed with E (n = 8, 6.3%) were identified among the 126 positive specimens. Multilocus sequence typing of G. duodenalis revealed 34 assemblage E multilocus genotypes (MLGs), 1 assemblage A MLG and 7 mixed assemblage (A and E) MLGs. The eBURST data showed a high degree of genetic diversity within assemblage E MLGs. The phylogenetic tree revealed that MLG E3 was the primary MLG subtype in Sichuan province and also the most widely distributed in China.
Klíčová slova:
Genetic loci – Phylogenetic analysis – Polymerase chain reaction – Zoonoses – China – DNA extraction – Farms – Giardia
Zdroje
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