Genome-wide histone modification profiling of inner cell mass and trophectoderm of bovine blastocysts by RAT-ChIP

English version

Autoři: Tõnis Org ^aff001; Kati Hensen ^aff001; Rita Kreevan ^aff001; Elina Mark ^aff002; Olav Sarv ^aff003; Reidar Andreson ^aff004; Ülle Jaakma ^aff002; Andres Salumets ^aff003; Ants Kurg ^aff001
Působiště autorů: Department of Biotechnology, Institute of Molecular and Cell Biology, University of Tartu, Tartu, Estonia ^aff001; Chair of Animal Breeding and Biotechnology, Estonian University of Life Sciences, Tartu, Estonia ^aff002; Competence Centre on Health Technologies, Tartu, Estonia ^aff003; Department of Bioinformatics, Institute of Molecular and Cell Biology, University of Tartu, Tartu, Estonia ^aff004; Institute of Genomics, University of Tartu, Tartu, Estonia ^aff005; Department of Obstetrics and Gynaecology, Institute of Clinical Medicine, University of Tartu, Tartu, Estonia ^aff006; Institute of Biomedicine and Translational Medicine, University of Tartu, Tartu, Estonia ^aff007; Department of Obstetrics and Gynaecology, University of Helsinki and Helsinki University Hospital, Helsinki, Finland ^aff008
Vyšlo v časopise: PLoS ONE 14(11)
Kategorie: Research Article
prolekare.web.journal.doi_sk: https://doi.org/10.1371/journal.pone.0225801

Souhrn

Chromatin immunoprecipitation coupled with next-generation sequencing (ChIP-seq) has revolutionized our understanding of chromatin-related biological processes. The method, however, requires thousands of cells and has therefore limited applications in situations where cell numbers are limited. Here we describe a novel method called Restriction Assisted Tagmentation Chromatin Immunoprecipitation (RAT-ChIP) that enables global histone modification profiling from as few as 100 cells. The method is simple, cost-effective and takes a single day to complete. We demonstrate the sensitivity of the method by deriving the first genome-wide maps of histone H3K4me3 and H3K27me3 modifications of inner cell mass and trophectoderm of bovine blastocyst stage embryos.

Klíčová slova:

Chromatin – Gene expression – Gene regulation – Embryos – Histones – Immunoprecipitation – Blastocysts – Histone modification

Zdroje

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Genome-wide histone modification profiling of inner cell mass and trophectoderm of bovine blastocysts by RAT-ChIP

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