Development of a novel automatable fabrication method based on electrospinning co electrospraying for rotator cuff augmentation patches
Autoři:
Sergi Rey-Vinolas aff001; Oscar Castaño aff001; Leonardo Ruiz-Macarrilla aff005; Xavier Llorens aff006; José M. Mora aff006; Elisabeth Engel aff001; Miguel A. Mateos-Timoneda aff001
Působiště autorů:
Biomaterials for Regenerative Therapies, Institute for Bioengineering of Catalonia (IBEC), The Barcelona Institute of Science and Technology (BIST), Barcelona, Spain
aff001; CIBER en Bioingeniería, Biomateriales y Nanomedicina (CIBER-BBN), Madrid, Spain
aff002; Serra Hunter Fellow, Electronics and Biomedical Engineering Department, University of Barcelona (UB), Barcelona, Spain
aff003; Bioelectronics Unit and Nanobioengineering Lab., Institute for Nanoscience and Nanotechnology of the University of Barcelona (IN2UB), Barcelona, Spain
aff004; Servei de C.O.T., Hospital Universitario Germans Trias i Pujol, Badalona, Spain
aff005; Fundació Joan Costa Roma, Consorci Sanitari de Terrassa, Terrassa, Spain
aff006; Servei de C.O.T., Hospital de Terrassa, Consorci Sanitari de Terrassa, Terrassa, Spain
aff007; Department of Materials Science and Metallurgical Engineering, EEBE campus, Technical University of Catalonia (UPC), Barcelona, Spain
aff008
Vyšlo v časopise:
PLoS ONE 14(11)
Kategorie:
Research Article
prolekare.web.journal.doi_sk:
https://doi.org/10.1371/journal.pone.0224661
Souhrn
Rotator cuff tear is one of the most common shoulder diseases. Rotator cuff augmentation (RCA) is trying to solve the high retear failure percentage after the surgery procedures (20–90%). The ideal augmentation patch must provide a temporal mechanical support during the healing process. In this work, we proposed a simple method for the fabrication of synthetic RCA patches. This method combines the use of electrospraying to produce poly-L-lactic-co-ε-caprolactone (PLC) films in an organogel form and electrospinning to produce poly(lactic) acid (PLA) nanofibers. The device consists in a combination of layers, creating a multilayered construct, enabling the possibility of tuning its mechanical properties and thickness. Besides, both techniques are simple to escalate for industrial production. A complete characterization has been performed to optimize the involved number of layers and production time of PLC films and PLA nanofibers fabrication, obtaining a final optimal configuration for RCA devices. Structural, mechanical and suture properties were evaluated. Also, the possibility of surface functionalization to improve the bioactivity of the scaffold was studied, adding aligned electrospun PLA nanofibers on the surface of the device to mimic the natural tendon topography. Surface modification was characterized by culturing adult normal human dermal fibroblasts. Lack of toxicity was detected for material presented, and cell alignment shape orientation guided by aligned fibers, mimicking tendon structure, was obtained. Cell proliferation and protein production were also evaluated.
Klíčová slova:
Tissue repair – Collagens – Tendons – Mechanical properties – Scanning electron microscopy – Cell metabolism – Nanomaterials – Thin films
Zdroje
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