Expression of Concern: The Role of the RACK1 Ortholog Cpc2p in Modulating Pheromone-Induced Cell Cycle Arrest in Fission Yeast
Authors:
Published in the journal:
PLoS ONE 14(11)
Category:
Expression of Concern
doi:
https://doi.org/10.1371/journal.pone.0225013
After this article [1] was published, concerns were raised about the following calcofluor white staining results reported in Figs 1, 2 and 5:
In the 8h, 16h, and 32h panels for JY546(cyr1-, sxa2-) in Fig 2A, and the 8h panel for JY1628(cyr1-, sxa2-, cpc2-) in Fig 2B, there appear to be discontinuities in the background image when levels are adjusted.
In the JY1578(cyr1-, sxa2-, oe-cpc2+) panels shown in Fig 2C, when levels are adjusted it appears that contrast levels differ between the cells and the background in the 0 hour, 8 hour and 16 hour panels. There is also a rectangle in the 0h panel and an area where background appears discontinuous with surrounding area in the 8h panel.
In Fig 5B, the image for JY948(cyr1-, sxa2-, pmp1-) appears to have different contrast levels between the cells and surrounding background.
The image for strain JY1716(sxa2-, pmp1-) in Fig 5B shows a strong similarity to the 16h panel in Fig 2C for strain JY1711(sxa2-,oe-cpc2+).
The image for strain JY710(sxa2-, pyp2-) in Fig 5D shows a strong similarity to the panel in Fig 1C for strain JY1711(sxa2-,oe-cpc2+).
The authors are unable to clarify the concerns raised about background irregularities in Fig 2A–2C but acknowledge the apparent discontinuities. The authors noted that in preparing Fig 2C the cell images were mounted on black backgrounds and a cell which was out of focus in the 0h panel was covered with an overlying rectangle. According to the authors, except for this instance, no cells were obscured or cropped out of the images. In the updated figure, the panels in question in Fig 2C have been replaced with alternative images from the original experiments; Fig 2A and 2B are unchanged. The underlying raw image for Fig 2A, JY546(cyr1-, sxa2-), 16h panel is provided in S1 File.
The authors apologize for the Fig 5 issues and noted that they were due to figure preparation errors. They commented that the image of JY948(cyr1-, sxa2-, pmp1-) cells in Fig 5B was placed on a black background in preparing the figure, but that the cell image accurately reflects data from the original slide. The incorrect data were included in Fig 5B, JY1716(sxa2-, pmp1-) panel, and in Fig 5D, JY710(sxa2-, pyp2-) and JY1717(sxa2-, pyp2-, cpc2+) panels. In the updated Fig 5, these panels are replaced with the correct images from the original experiments. The underlying raw image for the original Fig 5B, 1716(sxa2-, pmp1-) panel is included in S2 File.
Original data are no longer available for the other figure panels discussed above and other results reported in the article. Due to the nature and extent of image issues and lack of underlying image data for the figures in question, the PLOS ONE Editors issue this Expression of Concern.
Some of the above concerns were raised in 2014. The PLOS ONE Editors apologize for our delay in resolving this matter and thank the authors for replying promptly to journal queries.
Supporting information
S1 File [pdf]
Underlying image for , JY546(, ), 16h panel.
S2 File [pdf]
Underlying image for , 1716(, ) panel.
Zdroje
1. Mos M, Esparza-Franco MA, Godfrey EL, Richardson K, Davey J, Ladds G (2013) The Role of the RACK1 Ortholog Cpc2p in Modulating Pheromone-Induced Cell Cycle Arrest in Fission Yeast. PLoS ONE 8(7): e65927. https://doi.org/10.1371/journal.pone.0065927 23843946
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