Chitotetraose activates the fungal-dependent endosymbiotic signaling pathway in actinorhizal plant species
Autoři:
Mireille Chabaud aff001; Joëlle Fournier aff001; Lukas Brichet aff001; Iltaf Abdou-Pavy aff001; Leandro Imanishi aff002; Laurent Brottier aff003; Elodie Pirolles aff003; Valérie Hocher aff003; Claudine Franche aff004; Didier Bogusz aff004; Luis G. Wall aff002; Sergio Svistoonoff aff003; Hassen Gherbi aff003; David G. Barker aff001
Působiště autorů:
Laboratory of Plant-Microbe Interactions (INRA/CNRS/University of Toulouse), Castanet-Tolosan, France
aff001; Laboratory of Biochemistry, Microbiology and Soil Biological Interactions, Department of Science and Technology, National University of Quilmes, CONICET, Bernal, Argentina
aff002; Laboratory of Tropical and Mediterranean Symbioses (IRD/INRA/CIRAD/University of Montpellier/Supagro), Montpellier, France
aff003; Plant Diversity, Adaptation and Development (IRD/University of Montpellier), Montpellier, France
aff004
Vyšlo v časopise:
PLoS ONE 14(10)
Kategorie:
Research Article
prolekare.web.journal.doi_sk:
https://doi.org/10.1371/journal.pone.0223149
Souhrn
Mutualistic plant-microbe associations are widespread in natural ecosystems and have made major contributions throughout the evolutionary history of terrestrial plants. Amongst the most remarkable of these are the so-called root endosymbioses, resulting from the intracellular colonization of host tissues by either arbuscular mycorrhizal (AM) fungi or nitrogen-fixing bacteria that both provide key nutrients to the host in exchange for energy-rich photosynthates. Actinorhizal host plants, members of the Eurosid 1 clade, are able to associate with both AM fungi and nitrogen-fixing actinomycetes known as Frankia. Currently, little is known about the molecular signaling that allows these plants to recognize their fungal and bacterial partners. In this article, we describe the use of an in vivo Ca2+ reporter to identify symbiotic signaling responses to AM fungi in roots of both Casuarina glauca and Discaria trinervis, actinorhizal species with contrasting modes of Frankia colonization. This approach has revealed that, for both actinorhizal hosts, the short-chain chitin oligomer chitotetraose is able to mimic AM fungal exudates in activating the conserved symbiosis signaling pathway (CSSP) in epidermal root cells targeted by AM fungi. These results mirror findings in other AM host plants including legumes and the monocot rice. In addition, we show that chitotetraose is a more efficient elicitor of CSSP activation compared to AM fungal lipo-chitooligosaccharides. These findings reinforce the likely role of short-chain chitin oligomers during the initial stages of the AM association, and are discussed in relation to both our current knowledge about molecular signaling during Frankia recognition as well as the different microsymbiont root colonization mechanisms employed by actinorhizal hosts.
Klíčová slova:
chitin – Plants – Fungi – Genetically modified plants – Symbiosis – Legumes – Root hairs – Acetonitrile
Zdroje
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